{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T17:21:48Z","timestamp":1792084908108,"version":"4.4.0"},"reference-count":32,"publisher":"Walter de Gruyter GmbH","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,12,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_jpm-2017-0355_s_999_w2aab3b7c32b1b6b1aab1c16b1Aa\">\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Oxidative stress plays an important part in the pathophysiology of hypoxic-ischemic encephalopathy (HIE) and is reliably measured through prostanoids following lipid peroxidation of polyunsaturated fatty acids (PUFAs). The aim of the study is to measure oxidative stress in the prefrontal cortex, white matter and hippocampus in the brains of hypoxic-ischemic piglets treated with docosahexaenoic acid (DHA) and therapeutic hypothermia (TH) and investigate the additive effects of DHA on hypothermia by factorial design.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpm-2017-0355_s_998_w2aab3b7c32b1b6b1aab1c16b2Aa\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Fifty-five piglets were randomized as having severe global hypoxia (n=48) or not (sham, n=7). Hypoxic piglets were further randomized: vehicle (VEH), DHA, VEH+hypothermia (HT) or HT+DHA. A total of 5 mg\/kg DHA was given intravenously 210 min after the end of hypoxia. Brain tissues were analyzed using liquid chromatography triple quadrupole mass spectrometry technique (LC-MS). A two-way analysis of variance (ANOVA) was performed with DHA and HT as main effects.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpm-2017-0355_s_997_w2aab3b7c32b1b6b1aab1c16b3Aa\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>In the white matter, we found main effects of DHA on DH-isoprostanes (P=0.030) and a main effect of HT on F<jats:sub>4<\/jats:sub>-neuroprostanes (F<jats:sub>4<\/jats:sub>-NeuroPs) (P=0.007), F<jats:sub>2<\/jats:sub>-isoprostanes (F<jats:sub>2<\/jats:sub>-IsoPs) (P=0.043) and DH-isoprostanes (P=0.023).<\/jats:p>\n                  <jats:p>In the cortex, the ANOVA analysis showed the interactions of main effects between DHA and HT for neurofuranes (NeuroFs) (P=0.092) and DH-isoprostanes (P=0.015) as DHA significantly reduced lipid peroxidation in the absence of HT. DHA compared to VEH significantly reduced NeuroFs (P=0.019) and DH-isoprostanes (P=0.010). No differences were found in the hippocampus.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpm-2017-0355_s_996_w2aab3b7c32b1b6b1aab1c16b4Aa\">\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>After severe hypoxia, HT reduced lipid peroxidation in the white matter but not in the cortical gray matter. HT attenuated the reducing effect of DHA on lipid peroxidation in the cortex. Further studies are needed to determine whether DHA can be an effective add-on therapy for TH.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/jpm-2017-0355","type":"journal-article","created":{"date-parts":[[2018,8,15]],"date-time":"2018-08-15T07:58:48Z","timestamp":1534319928000},"page":"82-89","source":"Crossref","is-referenced-by-count":9,"title":["Regional differences of hypothermia on oxidative stress following hypoxia-ischemia: a study of DHA and hypothermia on brain lipid peroxidation in newborn piglets"],"prefix":"10.1515","volume":"47","author":[{"given":"Marianne U.","family":"Huun","sequence":"first","affiliation":[{"name":"Department of Pediatric Research , Institute of Surgical Research , University of Oslo, Oslo University Hospital Rikshospitalet, Postboks 4950 Nydalen , 0424 Oslo , Norway , Tel.: +47 97060117"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e5vard T.","family":"Garberg","sequence":"additional","affiliation":[{"name":"Department of Pediatric Research , Institute of Surgical Research , University of Oslo, Oslo University Hospital Rikshospitalet , Oslo , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Buonocore","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine , University of Siena , Siena , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariangela","family":"Longini","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine , University of Siena , Siena , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Belvisi","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine , University of Siena , Siena , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Bazzini","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine , University of Siena , Siena , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Proietti","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine , University of Siena , Siena , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ola Didrik","family":"Saugstad","sequence":"additional","affiliation":[{"name":"Department of Pediatric Research , Institute of Surgical Research , University of Oslo, Oslo University Hospital Rikshospitalet , Oslo , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00f8nnaug","family":"Solberg","sequence":"additional","affiliation":[{"name":"Department of Pediatric Research , Institute of Surgical Research , University of Oslo, Oslo University Hospital Rikshospitalet , Oslo , Norway"},{"name":"Department of Pediatrics , Vestfold Hospital Trust , T\u00f8nsberg , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2018,8,15]]},"reference":[{"key":"2025021114312555506_j_jpm-2017-0355_ref_001_w2aab3b7c32b1b6b1ab2b1b1Aa","doi-asserted-by":"crossref","unstructured":"Kumar A, Mittal R, Khanna HD, Basu S. 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