{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T11:58:54Z","timestamp":1779278334199,"version":"3.51.4"},"reference-count":28,"publisher":"Walter de Gruyter GmbH","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,5,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_jpm-2018-0305_s_999_w2aab2b8c34b1b7b1aab1c16b1Aa\">\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The pathogenesis of bronchopulmonary dysplasia (BPD) includes arrest of alveolar septation and enhanced fibrosis. We hypothesized that mesenchymal stromal cells (MSC) and transforming growth factor-\u03b21 (TGF-\u03b21) in tracheal aspirates of mechanically ventilated premature infants differ in BPD and non-BPD infants.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpm-2018-0305_s_998_w2aab2b8c34b1b7b1aab1c16b2Aa\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Tracheal aspirates were collected during the first week of life. Mononuclear cells were separated, cultured and immunophenotyped by flow cytometry. MSCs colony\/cluster ratio was calculated as an index for dysplastic potentials. TGF-\u03b21 was assessed by enzyme-linked immunosorbent assay (ELISA). Setting: Neonatal intensive care unit.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpm-2018-0305_s_997_w2aab2b8c34b1b7b1aab1c16b3Aa\">\n                  <jats:title>Patients<\/jats:title>\n                  <jats:p>Premature infants at risk for BPD.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpm-2018-0305_s_996_w2aab2b8c34b1b7b1aab1c16b4Aa\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>A total of 121 preterm infants were enrolled; 27 of them died and among the 94 survivors 23 infants had BPD. MSCs were identified in younger [gestational age (GA): 30.9\u00b11.7 vs. 31.8\u00b11.8, P=0.025] and smaller [birth weight (BW): 1.3\u00b10.28 vs. 1.44\u00b10.37 kg, P=0.04] infants with lower Apgar scores. The recovery rate of MSCs in BPD and non-BPD groups did not differ. BPD group had significantly smaller colony\/cluster ratio compared to non-BPD (0.97 vs. 4.25, P=0.002). TGF-\u03b21 was significantly greater in BPD infants (4173.9\u00b1864.3 vs. 3705.8\u00b1540.5 pg\/mL, P=0.021).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpm-2018-0305_s_995_w2aab2b8c34b1b7b1aab1c16b5Aa\">\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Infants with BPD had different MSCs morphology and greater TGF-\u03b21 expression. The pathogenesis for these morphological changes of resident lung MSCs needs further studying.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/jpm-2018-0305","type":"journal-article","created":{"date-parts":[[2019,5,6]],"date-time":"2019-05-06T19:42:20Z","timestamp":1557171740000},"page":"470-477","source":"Crossref","is-referenced-by-count":11,"title":["Mesenchymal stromal cells and TGF-\u03b21 in tracheal aspirate of premature infants: early predictors for bronchopulmonary dysplasia?"],"prefix":"10.1515","volume":"47","author":[{"given":"Hany","family":"Aly","sequence":"first","affiliation":[{"name":"Department of Neonatology , Cleveland Clinic Children\u2019s Hospital , 9500 Euclid Avenue, M31-37 , Cleveland, OH 44195 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasmeen","family":"Mansi","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Department of Pediatrics , Cairo University Children\u2019s Hospital , Cairo , Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahraa","family":"Ez El Din","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Department of Pediatrics , Cairo University Children\u2019s Hospital , Cairo , Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hala","family":"Gabr Metwally","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Pediatrics , Cairo University Children\u2019s Hospital , Cairo , Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amira","family":"Sabry","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Department of Pediatrics , Cairo University Children\u2019s Hospital , Cairo , Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2019,2,21]]},"reference":[{"key":"2023033121263850346_j_jpm-2018-0305_ref_001_w2aab2b8c34b1b7b1ab2b1b1Aa","doi-asserted-by":"crossref","unstructured":"Wright CJ, Kirpalani H. 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