{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T04:06:52Z","timestamp":1773806812635,"version":"3.50.1"},"reference-count":23,"publisher":"Walter de Gruyter GmbH","issue":"3","license":[{"start":{"date-parts":[[2021,12,7]],"date-time":"2021-12-07T00:00:00Z","timestamp":1638835200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Czech Health Research Council Project","award":["NV17-31403A"],"award-info":[{"award-number":["NV17-31403A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_jpm-2021-0184_abs_001\">\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The aim of this study is to evaluate the diagnostic ability of multiplex real-time polymerase chain reaction (PCR) in very preterm infants assessed for risk of early onset neonatal sepsis (EOS).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpm-2021-0184_abs_002\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Prospective observational cohort study. Blood samples of preterm neonates\u00a0\u226432\u00a0weeks of gestation were evaluated by commercial multiplex real-time PCR within 2\u00a0h after delivery. The definition of EOS was based on positive blood culture and clinical signs of infection or negative blood culture, clinical signs of infection and abnormal neonatal blood count and serum biomarkers.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpm-2021-0184_abs_003\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Among 82 subjects analyzed in the study, 15 had clinical or confirmed EOS. PCR was positive in four of these infants (including the only one with a positive blood culture), as well as in 15 of the 67 infants without sepsis (sensitivity 27%, specificity 78%). Out of 19 PCR positive subjects, <jats:italic>Escherichia coli<\/jats:italic> was detected in 12 infants (63%). Statistically significant association was found between vaginal <jats:italic>E.\u00a0coli<\/jats:italic> colonization of the mother and <jats:italic>E.\u00a0coli<\/jats:italic> PCR positivity of the neonate (p=0.001). No relationship was found between neonatal <jats:italic>E.\u00a0coli<\/jats:italic> swab results and assessment findings of bacterial DNA in neonatal blood stream.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jpm-2021-0184_abs_004\">\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Multiplex real-time PCR had insufficient diagnostic capability for EOS in high risk very preterm infants. The study revealed no significant association between PCR results and the diagnosis of clinical EOS. Correlation between maternal vaginal swab results and positive PCR in the newborn needs further investigation to fully understand the role of bacterial DNA analysis in preterm infants.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/jpm-2021-0184","type":"journal-article","created":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T10:15:05Z","timestamp":1639044905000},"page":"356-362","source":"Crossref","is-referenced-by-count":6,"title":["Bacterial DNA detection in very preterm infants assessed for risk of early onset sepsis"],"prefix":"10.1515","volume":"50","author":[{"given":"Zbyn\u011bk","family":"Stra\u0148\u00e1k","sequence":"first","affiliation":[{"name":"Institute for the Care of Mother and Child , Prague , Czech Republic"},{"name":"Third Faculty of Medicine, Charles University , Prague , Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Berka","sequence":"additional","affiliation":[{"name":"Institute for the Care of Mother and Child , Prague , Czech Republic"},{"name":"Third Faculty of Medicine, Charles University , Prague , Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6166-3842","authenticated-orcid":false,"given":"Peter","family":"Kor\u010dek","sequence":"additional","affiliation":[{"name":"Institute for the Care of Mother and Child , Prague , Czech Republic"},{"name":"Third Faculty of Medicine, Charles University , Prague , Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Urb\u00e1nek","sequence":"additional","affiliation":[{"name":"Institute for the Care of Mother and Child , Prague , Czech Republic"},{"name":"Third Faculty of Medicine, Charles University , Prague , Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00e1\u0148a","family":"L\u00e1zni\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Synlab , Prague , Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Libor","family":"Stan\u011bk","sequence":"additional","affiliation":[{"name":"Department of Microbiology , Synlab , Prague , Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2021,12,7]]},"reference":[{"key":"2023040100552180738_j_jpm-2021-0184_ref_001","doi-asserted-by":"crossref","unstructured":"Fleischmann-Struzek, C, Goldfarb, DM, Schlattmann, P, Schlapbach, LJ, Reinhart, K, Kissoon, N. The global burden of paediatric and neonatal sepsis: a systematic review. Lancet Respir Med 2018;6:223\u201330. https:\/\/doi.org\/10.1016\/s2213-2600(18)30063-8.","DOI":"10.1016\/S2213-2600(18)30063-8"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_002","doi-asserted-by":"crossref","unstructured":"Molloy, EJ, Wynn, JL, Bliss, J, Koenig, JM, Keij, FM, McGovern, M, et al.. Neonatal sepsis: need for consensus definition, collaboration and core outcomes. Pediatr Res 2020;88:2\u20134. https:\/\/doi.org\/10.1038\/s41390-020-0850-5.","DOI":"10.1038\/s41390-020-0850-5"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_003","doi-asserted-by":"crossref","unstructured":"Iroh Tam, PY, Bendel, CM. Diagnostics for neonatal sepsis: current approaches and future directions. Pediatr Res 2017;82:574\u201383. https:\/\/doi.org\/10.1038\/pr.2017.134.","DOI":"10.1038\/pr.2017.134"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_004","doi-asserted-by":"crossref","unstructured":"Shane, AL, S\u00e1nchez, PJ, Stoll, BJ. Neonatal sepsis. Lancet 2017;390:1770\u201380. https:\/\/doi.org\/10.1016\/s0140-6736(17)31002-4.","DOI":"10.1016\/S0140-6736(17)31002-4"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_005","doi-asserted-by":"crossref","unstructured":"Benitz, WE, Wynn, JL, Polin, RA. Reappraisal of guidelines for management of neonates with suspected early-onset sepsis. J Pediatr 2015;166:1070\u20134. https:\/\/doi.org\/10.1016\/j.jpeds.2014.12.023.","DOI":"10.1016\/j.jpeds.2014.12.023"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_006","doi-asserted-by":"crossref","unstructured":"van den Hoogen, A, Gerards, LJ, Verboon-Maciolek, MA, Fleer, A, Krediet, TG. Long-term trends in the epidemiology of neonatal sepsis and antibiotic susceptibility of causative agents. Neonatology 2010;97:22\u20138. https:\/\/doi.org\/10.1159\/000226604.","DOI":"10.1159\/000226604"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_007","doi-asserted-by":"crossref","unstructured":"Klingenberg, C, Kornelisse, RF, Buonocore, G, Maier, RF, Stocker, M. Culture-negative early-onset neonatal sepsis\u00a0\u2013 at the crossroad between efficient sepsis care and antimicrobial stewardship. Front Pediatr 2018;6:285. https:\/\/doi.org\/10.3389\/fped.2018.00285.","DOI":"10.3389\/fped.2018.00285"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_008","doi-asserted-by":"crossref","unstructured":"Wynn, JL, Polin, RA. Progress in the management of neonatal sepsis: the importance of a consensus definition. Pediatr Res 2018;83:13\u20135. https:\/\/doi.org\/10.1038\/pr.2017.224.","DOI":"10.1038\/pr.2017.224"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_009","doi-asserted-by":"crossref","unstructured":"Schlapbach, LJ, Kissoon, N. Defining pediatric sepsis. JAMA Pediatr 2018;172:312\u20134. https:\/\/doi.org\/10.1001\/jamapediatrics.2017.5208.","DOI":"10.1001\/jamapediatrics.2017.5208"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_010","doi-asserted-by":"crossref","unstructured":"Procianoy, RS, Silveira, RC. The challenges of neonatal sepsis management. J Pediatr 2020;96:80\u20136. https:\/\/doi.org\/10.1016\/j.jped.2019.10.004.","DOI":"10.1016\/j.jped.2019.10.004"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_011","doi-asserted-by":"crossref","unstructured":"Espy, MJ, Uhl, JR, Sloan, LM, Buckwalter, SP, Jones, MF, Vetter, EA, et al.. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin Microbiol Rev 2006;19:165\u2013256. https:\/\/doi.org\/10.1128\/cmr.19.1.165-256.2006.","DOI":"10.1128\/CMR.19.1.165-256.2006"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_012","doi-asserted-by":"crossref","unstructured":"Pammi, M, Flores, A, Leeflang, M, Versalovic, J. Molecular assays in the diagnosis of neonatal sepsis: a systematic review and meta-analysis. Pediatrics 2011;128:e973\u201385. https:\/\/doi.org\/10.1542\/peds.2011-1208.","DOI":"10.1542\/peds.2011-1208"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_013","doi-asserted-by":"crossref","unstructured":"Oeser, C, Pond, M, Butcher, P, Bedford Russell, A, Henneke, P, Laing, K, et al.. PCR for the detection of pathogens in neonatal early onset sepsis. PLoS One 2020;15:e0226817. https:\/\/doi.org\/10.1371\/journal.pone.0226817.","DOI":"10.1371\/journal.pone.0226817"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_014","doi-asserted-by":"crossref","unstructured":"Kim, CJ, Romero, R, Chaemsaithong, P, Chaiyasit, N, Yoon, BH, Kim, YM. Acute chorioamnionitis and funisitis: definition, pathologic features, and clinical significance. Am J Obstet Gynecol 2015;213:S29\u201352. https:\/\/doi.org\/10.1016\/j.ajog.2015.08.040.","DOI":"10.1016\/j.ajog.2015.08.040"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_015","doi-asserted-by":"crossref","unstructured":"Janota, J, Stran\u00e1k, Z, B\u0115lohl\u00e1vkov\u00e1, S, Mudra, K, Sim\u00e1k, J. Postnatal increase of procalcitonin in premature newborns is enhanced by chorioamnionitis and neonatal sepsis. Eur J Clin Invest 2001;31:978\u201383. https:\/\/doi.org\/10.1046\/j.1365-2362.2001.00912.x.","DOI":"10.1046\/j.1365-2362.2001.00912.x"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_016","doi-asserted-by":"crossref","unstructured":"Chiesa, C, Pellegrini, G, Panero, A, Osborn, JF, Signore, F, Assumma, M, et al.. C-reactive protein, interleukin-6, and procalcitonin in the immediate postnatal period: influence of illness severity, risk status, antenatal and perinatal complications, and infection. Clin Chem 2003;49:60\u20138. https:\/\/doi.org\/10.1373\/49.1.60.","DOI":"10.1373\/49.1.60"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_017","doi-asserted-by":"crossref","unstructured":"Simonsen, KA, Anderson-Berry, AL, Delair, SF, Davies, HD. Early-onset neonatal sepsis. Clin Microbiol Rev 2014;27:21\u201347. https:\/\/doi.org\/10.1128\/cmr.00031-13.","DOI":"10.1128\/CMR.00031-13"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_018","doi-asserted-by":"crossref","unstructured":"Puopolo, K, Benitz, WE, Zaoutis, TE, AAP Committee on Fetus and Newborn, AAP Committee on Infectious Diseases. Management of neonates born\u00a0\u226434\u00a06\/7\u00a0weeks gestation with suspected or proven early-onset bacterial sepsis. Pediatrics 2018;142:e20182896. https:\/\/doi.org\/10.1542\/peds.2018-2896.","DOI":"10.1542\/9781610023047-part05-management_of_neonates"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_019","doi-asserted-by":"crossref","unstructured":"Horbar, JD. The Vermont Oxford Network: evidence-based quality improvement for neonatology. Pediatrics 1999;103:350\u20139.","DOI":"10.1542\/peds.103.SE1.350"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_020","doi-asserted-by":"crossref","unstructured":"Pammi, M, Flores, A, Versalovic, J, Leeflang, MM. Molecular assays for the diagnosis of sepsis in neonates. Cochrane Database Syst Rev 2017;2:CD011926. https:\/\/doi.org\/10.1002\/14651858.CD011926.pub2.","DOI":"10.1002\/14651858.CD011926.pub2"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_021","doi-asserted-by":"crossref","unstructured":"Cantey, JB, Baird, SD. Ending the culture of culture-negative sepsis in the neonatal ICU. Pediatrics 2017;140:e20170044. https:\/\/doi.org\/10.1542\/peds.2017-0044.","DOI":"10.1542\/peds.2017-0044"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_022","doi-asserted-by":"crossref","unstructured":"Bustin, SA, Benes, V, Garson, JA, Hellemans, J, Huggett, J, Kubista, M, et al.. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009;55:611\u201322. https:\/\/doi.org\/10.1373\/clinchem.2008.112797.","DOI":"10.1373\/clinchem.2008.112797"},{"key":"2023040100552180738_j_jpm-2021-0184_ref_023","doi-asserted-by":"crossref","unstructured":"Delc\u00f2, C, Karam, O, Pfister, R, Gervaix, A, Renzi, G, Emonet, S, et al.. Rapid detection and ruling out of neonatal sepsis by PCR coupled with Electrospray Ionization Mass Spectrometry (PCR\/ESI-MS). Early Hum Dev 2017;108:17\u201322. https:\/\/doi.org\/10.1016\/j.earlhumdev.2017.03.004.","DOI":"10.1016\/j.earlhumdev.2017.03.004"}],"container-title":["Journal of Perinatal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpm-2021-0184\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpm-2021-0184\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,1]],"date-time":"2023-04-01T17:12:23Z","timestamp":1680369143000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/jpm-2021-0184\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,7]]},"references-count":23,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,12,16]]},"published-print":{"date-parts":[[2022,3,28]]}},"alternative-id":["10.1515\/jpm-2021-0184"],"URL":"https:\/\/doi.org\/10.1515\/jpm-2021-0184","relation":{},"ISSN":["0300-5577","1619-3997"],"issn-type":[{"value":"0300-5577","type":"print"},{"value":"1619-3997","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,7]]}}}