{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T09:24:47Z","timestamp":1766222687383,"version":"3.48.0"},"reference-count":25,"publisher":"Walter de Gruyter GmbH","license":[{"start":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T00:00:00Z","timestamp":1754611200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_jpm-2025-0102_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The objective of this study was to examine the role of microRNA-203a-3p (miR-203a-3p) in the pathogenesis of necrotizing enterocolitis (NEC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jpm-2025-0102_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Quantitative real-time polymerase chain reaction (qRT-PCR) and receiver operating characteristic (ROC) curve analysis were employed to evaluate the relative abundances of miR-203a-3p as well as its diagnostic capacity. Logistic regression was applied to search for influential risk factors associated with NEC in neonates. Cell behaviors were assessed with flow cytometry and CCK-8 detection. The target genes of miR-203a-3p and its potential biological functions were analyzed via bioinformatic analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jpm-2025-0102_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A significant reduction in serum levels of miR-203a-3p was observed in neonates with NEC. Notably, this miRNA exhibited exceptional diagnostic precision for differentiating NEC from non-NEC cases, as evidenced by an area under the curve (AUC) of 0.928. Furthermore, miR-203a-3p was established as an independent indicator for assessing the severity of NEC. In an NEC cell model, levels of miR-203a-3p were distinctly diminished; however, this decrease was significantly reversed following transfection with miR-203a-3p (p&lt;0.001). Correspondingly, findings were noted regarding cell apoptosis, cell viability, inflammatory indicators, and antioxidant enzyme activities. MiR-203a-3p-related genes predominantly clustered within inflammatory-associated signaling pathways and proteins, particularly ataxia telangiectasia mutated (ATM). Notably, miR-203a-3p was found to directly target ATM. Importantly, heightened levels of ATM were detected in both neonates with NEC and LPS-triggered fetal human colon (FHC) cells (p&lt;0.001).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jpm-2025-0102_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>MiR-203a-3p alleviates LPS-induced inflammatory damage in FHC cells through regulating ATM, thereby presenting a promising avenue for the development of novel therapeutic strategies for neonates with\u00a0NEC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/jpm-2025-0102","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T10:03:23Z","timestamp":1754561003000},"source":"Crossref","is-referenced-by-count":0,"title":["Correlation of serum miR-203a-3p with the severity of neonates with necrotizing enterocolitis and analysis of its molecular mechanism"],"prefix":"10.1515","author":[{"given":"Fengna","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Neonatology , 477167 Maternal and Child Health Hospital of Hubei Province , Wuhan , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengjiang","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Laboratory, Maternal and Child Health Hospital of Hubei Province , Wuhan , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neonatology , 477167 Maternal and Child Health Hospital of Hubei Province , Wuhan , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neonatology , 477167 Maternal and Child Health Hospital of Hubei Province , Wuhan , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2025,8,8]]},"reference":[{"key":"2025122009100267872_j_jpm-2025-0102_ref_001","doi-asserted-by":"crossref","unstructured":"Choi, G, Je, BK, Kim, YJ. 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