{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T03:00:27Z","timestamp":1781319627637,"version":"3.54.1"},"reference-count":28,"publisher":"Walter de Gruyter GmbH","issue":"5","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG BA 7308\/4-1"],"award-info":[{"award-number":["DFG BA 7308\/4-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_jpm-2025-0577_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Animal models suggest that combining mesenchymal stromal cells (MSCs) with prenatal myelomeningocele (MMC) repair may help preserve spinal cord function. This study assessed the feasibility of administration human umbilical cord-derived MSCs during prenatal repair of MMC in a sheep\u00a0model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jpm-2025-0577_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      MMC (L1\u2013L5) were surgically created in 11 fetal lambs at 75\u00a0days. Seven underwent repair 25\u00a0days later\u00a0\u2013\u00a0five with and two without MSCs. A high-dose, ready-to-use MSCs product (Desacell\n                      <jats:sup>\u00ae<\/jats:sup>\n                      ), derived from healthy human neonatal umbilical cord tissue was administered in five cases. Each received 1\u202fmL of Desacell\n                      <jats:sup>\u00ae<\/jats:sup>\n                      gel containing 30 million MSCs directly to the spinal cord. All fetuses underwent multilayer dural myofascial patching and skin closure.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jpm-2025-0577_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the seven treated lambs, three (two with MSCs, one without) survived to term (144\u2013147\u00a0days gestation). Motor function was evaluated using the sheep locomotor rating scale before euthanasia. MMC sites were excised en-bloc. Mean large neuron densities in gray matter were 20.4, 15, and 13.1 per\u00a0mm\n                      <jats:sup>2<\/jats:sup>\n                      , respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jpm-2025-0577_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This is the first report of successful local administration of MSCs during\n                      <jats:italic>in utero<\/jats:italic>\n                      MMC repair in a sheep model using Desacell\n                      <jats:sup>\u00ae<\/jats:sup>\n                      , a ready-to-use stem cell product. MSCs administration was feasible in all intended cases, however, the limited sample size restricted comparative outcomes analyses.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/jpm-2025-0577","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T14:58:53Z","timestamp":1771599533000},"page":"881-888","source":"Crossref","is-referenced-by-count":0,"title":["<i>In vivo<\/i>\n                    therapy for prenatal repair of the ovine fetal myelomeningocele model using human umbilical cord-derived mesenchymal stem cells: proof of principle"],"prefix":"10.1515","volume":"54","author":[{"given":"Christian","family":"Bamberg","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Fetal Medicine , University Medical Center Hamburg-Eppendorf , Hamburg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Tomuschat","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery , University Medical Center Hamburg-Eppendorf , Hamburg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt","family":"Hecher","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Fetal Medicine , University Medical Center Hamburg-Eppendorf , Hamburg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"R\u00fcdiger","sequence":"additional","affiliation":[{"name":"Saxonian Center for Feto\/Neonatal Health , Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden , Dresden , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marius A.","family":"M\u00f6bius","sequence":"additional","affiliation":[{"name":"Saxonian Center for Feto\/Neonatal Health , Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universit\u00e4t Dresden , Dresden , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Hagel","sequence":"additional","affiliation":[{"name":"Institute of Neuropathology, University Medical Center Hamburg-Eppendorf , Hamburg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Reitmeier","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Science , University Medical Center Hamburg-Eppendorf , Hamburg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Luise","family":"Schr\u00f6der","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animal Science , University Medical Center Hamburg-Eppendorf , Hamburg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke","family":"Diemert","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Fetal Medicine , University Medical Center Hamburg-Eppendorf , Hamburg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konrad","family":"Reinshagen","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery , University Medical Center Hamburg-Eppendorf , Hamburg , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"2026061302442414193_j_jpm-2025-0577_ref_001","unstructured":"European Commission, Joint Research, Centre. EUROCAT data: prevalence charts and tables [Internet]. European Platform on Rare Disease Registration; 2025. https:\/\/eu-rd-platform.jrc.ec.europa.eu\/eurocat\/eurocat-data\/prevalence_en [Accessed 14 Feb 2026]."},{"key":"2026061302442414193_j_jpm-2025-0577_ref_002","doi-asserted-by":"crossref","unstructured":"Bahlmann, F, Reinhard, I, Schramm, T, Geipel, A, Gembruch, U, von Kaisenberg, CS, et al.. Cranial and cerebral signs in the diagnosis of spina bifida between 18 and 22\u00a0weeks of gestation: a German multicentre study. Prenat Diagn 2015;35:228\u201335. https:\/\/doi.org\/10.1002\/pd.4524.","DOI":"10.1002\/pd.4524"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_003","doi-asserted-by":"crossref","unstructured":"Adzick, NS, Thom, EA, Spong, CY, Brock, JW3rd, Burrows, PK, Johnson, MP, et al.. A randomized trial of prenatal versus postnatal repair of myelomeningocele. N\u00a0Engl J Med 2011;364:993\u20131004. https:\/\/doi.org\/10.1056\/nejmoa1014379.","DOI":"10.1056\/NEJMoa1014379"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_004","doi-asserted-by":"crossref","unstructured":"Athiel, Y, Jouannic, JM, Mauffre, V, Dehan, C, Adam, C, Blot, S, et al.. Allogenic umbilical cord-derived mesenchymal stromal cells improve motor function in prenatal surgical repair of myelomeningocele: an ovine model study. BJOG 2024;131:759\u201367. https:\/\/doi.org\/10.1111\/1471-0528.17624.","DOI":"10.1111\/1471-0528.17624"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_005","doi-asserted-by":"crossref","unstructured":"Guilbaud, L, Dugas, A, Weber, M, Deflers, C, Lallemant, P, Lilin, T, et al.. In utero treatment of myelomeningocele with allogenic umbilical cord-derived mesenchymal stromal cells in an ovine model. Curr Res Transl Med 2022;70:103314. https:\/\/doi.org\/10.1016\/j.retram.2021.103314.","DOI":"10.1016\/j.retram.2021.103314"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_006","doi-asserted-by":"crossref","unstructured":"Kabagambe, S, Keller, B, Becker, J, Goodman, L, Pivetti, C, Lankford, L, et al.. Placental mesenchymal stromal cells seeded on clinical grade extracellular matrix improve ambulation in ovine myelomeningocele. J\u00a0Pediatr Surg 2018;53:178\u201382. https:\/\/doi.org\/10.1016\/j.jpedsurg.2017.10.032.","DOI":"10.1016\/j.jpedsurg.2017.10.032"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_007","doi-asserted-by":"crossref","unstructured":"Papanna, R, Moise, KJJr., Mann, LK, Fletcher, S, Schniederjan, R, Bhattacharjee, MB, et al.. Cryopreserved human umbilical cord patch for in-utero spina bifida repair. Ultrasound Obstet Gynecol 2016;47:168\u201376. https:\/\/doi.org\/10.1002\/uog.15790.","DOI":"10.1002\/uog.15790"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_008","doi-asserted-by":"crossref","unstructured":"Theodorou, CM, Stokes, SC, Jackson, JE, Pivetti, CD, Kumar, P, Yamashiro, KJ, et al.. Efficacy of clinical-grade human placental mesenchymal stromal cells in fetal ovine myelomeningocele repair. J\u00a0Pediatr Surg 2022;57:753\u20138.","DOI":"10.1016\/j.jpedsurg.2021.05.025"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_009","doi-asserted-by":"crossref","unstructured":"Vanover, M, Pivetti, C, Lankford, L, Kumar, P, Galganski, L, Kabagambe, S, et al.. High density placental mesenchymal stromal cells provide neuronal preservation and improve motor function following in utero treatment of ovine myelomeningocele. J\u00a0Pediatr Surg 2019;54:75\u20139. https:\/\/doi.org\/10.1016\/j.jpedsurg.2018.10.032.","DOI":"10.1016\/j.jpedsurg.2018.10.032"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_010","doi-asserted-by":"crossref","unstructured":"Wang, A, Brown, EG, Lankford, L, Keller, BA, Pivetti, CD, Sitkin, NA, et al.. Placental mesenchymal stromal cells rescue ambulation in ovine myelomeningocele. Stem Cells Transl Med 2015;4:659\u201369. https:\/\/doi.org\/10.5966\/sctm.2014-0296.","DOI":"10.5966\/sctm.2014-0296"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_011","doi-asserted-by":"crossref","unstructured":"Joyeux, L, Engels, AC, Van Der Merwe, J, Aertsen, M, Patel, PA, Deprez, M, et al.. Validation of the fetal lamb model of bifida. Sci Rep 2019;9:9327. https:\/\/doi.org\/10.1038\/s41598-019-45819-3.","DOI":"10.1038\/s41598-019-45819-3"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_012","doi-asserted-by":"crossref","unstructured":"Thompson, M, Mei, SHJ, Wolfe, D, Champagne, J, Fergusson, D, Stewart, DJ, et al.. Cell therapy with intravascular administration of mesenchymal stromal cells continues to appear safe: an updated systematic review and meta-analysis. eClinicalMedicine 2020;19:100249. https:\/\/doi.org\/10.1016\/j.eclinm.2019.100249.","DOI":"10.1016\/j.eclinm.2019.100249"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_013","doi-asserted-by":"crossref","unstructured":"Brown, EG, Keller, BA, Pivetti, CD, Sitkin, NA, Wang, A, Farmer, DL, et al.. Development of a locomotor rating scale for testing motor function in sheep. J\u00a0Pediatr Surg 2015;50:617\u201321. https:\/\/doi.org\/10.1016\/j.jpedsurg.2015.01.002.","DOI":"10.1016\/j.jpedsurg.2015.01.002"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_014","doi-asserted-by":"crossref","unstructured":"Meuli, M, Meuli-Simmen, C, Hutchins, GM, Yingling, CD, Hoffman, KM, Harrison, MR, et al.. In utero surgery rescues neurological function at birth in sheep with spina bifida. Nat Med 1995;1:342\u20137. https:\/\/doi.org\/10.1038\/nm0495-342.","DOI":"10.1038\/nm0495-342"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_015","doi-asserted-by":"crossref","unstructured":"Stokes, SC, Theodorou, CM, Jackson, JE, Pivetti, C, Kumar, P, Yamashiro, KJ, et al.. Long-term safety evaluation of placental mesenchymal stromal cells for in utero repair of myelomeningocele in a novel ovine model. J\u00a0Pediatr Surg 2022;57:18\u201325. https:\/\/doi.org\/10.1016\/j.jpedsurg.2021.09.021.","DOI":"10.1016\/j.jpedsurg.2021.09.021"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_016","doi-asserted-by":"crossref","unstructured":"Athiel, Y, Nasone, J, Arakelian, L, Faivre, L, Dugas, A, Jouannic, JM, et al.. Biodistribution of allogenic umbilical cord-derived mesenchymal stromal cells after fetal repair of myelomeningocele in an ovine model. Stem Cell Res Ther 2022;13:300. https:\/\/doi.org\/10.1186\/s13287-022-02991-0.","DOI":"10.1186\/s13287-022-02991-0"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_017","doi-asserted-by":"crossref","unstructured":"Mann, LK, Won, JH, Garnett, J, Patel, RP, Narayana, PA, Jain, R, et al.. Cryopreserved human umbilical cord as a meningeal patch during in utero spina bifida repair in a modified ovine model. J\u00a0Neurosurg 2023;139:1169\u201379. https:\/\/doi.org\/10.3171\/2023.2.jns222928.","DOI":"10.3171\/2023.2.JNS222928"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_018","doi-asserted-by":"crossref","unstructured":"Spoor, JKH, Kik, CC, van Veelen, MC, Dirven, C, Miller, JL, Groves, ML, et al.. Potential higher risk of tethered spinal cord in children after prenatal surgery for myelomeningocele: a systematic review and meta-analysis. PLoS One 2023;18:e0287175. https:\/\/doi.org\/10.1371\/journal.pone.0287175.","DOI":"10.1371\/journal.pone.0287175"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_019","doi-asserted-by":"crossref","unstructured":"Kunpalin, Y, Subramaniam, S, Perin, S, Gerli, MFM, Bosteels, J, Ourselin, S, et al.. Preclinical stem cell therapy in fetuses with myelomeningocele: a systematic review and meta-analysis. Prenat Diagn 2021;41:283\u2013300. https:\/\/doi.org\/10.1002\/pd.5887.","DOI":"10.1002\/pd.5887"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_020","doi-asserted-by":"crossref","unstructured":"Dugas, A, Larghero, J, Zerah, M, Jouannic, JM, Guilbaud, L. Cell therapy for prenatal repair of myelomeningocele: a systematic review. Curr Res Transl Med 2020;68:183\u20139. https:\/\/doi.org\/10.1016\/j.retram.2020.04.004.","DOI":"10.1016\/j.retram.2020.04.004"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_021","doi-asserted-by":"crossref","unstructured":"Lankford, L, Selby, T, Becker, J, Ryzhuk, V, Long, C, Farmer, D, et al.. Early gestation chorionic villi-derived stromal cells for fetal tissue engineering. World J Stem Cell 2015;7:195\u2013207. https:\/\/doi.org\/10.4252\/wjsc.v7.i1.195.","DOI":"10.4252\/wjsc.v7.i1.195"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_022","doi-asserted-by":"crossref","unstructured":"Athiel, Y, Cariot, L, Jouannic, JM, Maillet, C, Mauffre, V, Adam, C, et al.. Safety and efficacy of human umbilical cord-derived mesenchymal stromal cells in fetal ovine myelomeningocele repair. Stem Cell Res Ther 2024;15:444. https:\/\/doi.org\/10.1186\/s13287-024-03991-y.","DOI":"10.1186\/s13287-024-03991-y"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_023","doi-asserted-by":"crossref","unstructured":"Hum, C, Tahir, U, Mei, SHJ, Champagne, J, Fergusson, DA, Lalu, M, et al.. Efficacy and safety of umbilical cord-derived mesenchymal stromal cell therapy in preclinical models of sepsis: a systematic review and meta-analysis. Stem Cells Transl Med 2024;13:346\u201361. https:\/\/doi.org\/10.1093\/stcltm\/szae003.","DOI":"10.1093\/stcltm\/szae003"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_024","doi-asserted-by":"crossref","unstructured":"Mobius, MA, Seidner, SR, McCurnin, DC, Menschner, L, Furboter-Behnert, I, Schonfeld, J, et al.. Prophylactic administration of mesenchymal stromal cells does not prevent arrested lung development in extremely premature-born non-human Primates. Stem Cells Transl Med 2023;12:97\u2013111. https:\/\/doi.org\/10.1093\/stcltm\/szac088.","DOI":"10.1093\/stcltm\/szac088"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_025","doi-asserted-by":"crossref","unstructured":"Levine, GJ, Levine, JM, Budke, CM, Kerwin, SC, Au, J, Vinayak, A, et al.. Description and repeatability of a newly developed spinal cord injury scale for dogs. Prev Vet Med 2009;89:121\u20137. https:\/\/doi.org\/10.1016\/j.prevetmed.2009.02.016.","DOI":"10.1016\/j.prevetmed.2009.02.016"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_026","doi-asserted-by":"crossref","unstructured":"Papanna, R, Mann, LK, Snowise, S, Morales, Y, Prabhu, SP, Tseng, SC, et al.. Neurological outcomes after human umbilical cord patch for in utero spina bifida repair in a sheep model. AJP Rep 2016;6:e309\u201317. https:\/\/doi.org\/10.1055\/s-0036-1592316.","DOI":"10.1055\/s-0036-1592316"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_027","doi-asserted-by":"crossref","unstructured":"Galganski, LA, Yamashiro, KJ, Pivetti, CD, Keller, BA, Becker, JC, Brown, EG, et al.. A decade of experience with the ovine model of myelomeningocele: risk factors for fetal loss. Fetal Diagn Ther 2020;47:507\u201313. https:\/\/doi.org\/10.1159\/000505400.","DOI":"10.1159\/000505400"},{"key":"2026061302442414193_j_jpm-2025-0577_ref_028","doi-asserted-by":"crossref","unstructured":"Vanover, M, Wang, A, Farmer, D. Potential clinical applications of placental stem cells for use in fetal therapy of birth defects. Placenta 2017;59:107\u201312. https:\/\/doi.org\/10.1016\/j.placenta.2017.05.010.","DOI":"10.1016\/j.placenta.2017.05.010"}],"container-title":["Journal of Perinatal Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/jpm-2025-0577\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/jpm-2025-0577\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T02:46:16Z","timestamp":1781318776000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/jpm-2025-0577\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,23]]},"references-count":28,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,2,16]]},"published-print":{"date-parts":[[2026,6,24]]}},"alternative-id":["10.1515\/jpm-2025-0577"],"URL":"https:\/\/doi.org\/10.1515\/jpm-2025-0577","relation":{},"ISSN":["0300-5577","1619-3997"],"issn-type":[{"value":"0300-5577","type":"print"},{"value":"1619-3997","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,23]]}}}