{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T08:34:24Z","timestamp":1774168464334,"version":"3.50.1"},"reference-count":34,"publisher":"Walter de Gruyter GmbH","issue":"3","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec id=\"j_jtim-2025-0021_s_007\">\n                  <jats:title>Background and Objectives<\/jats:title>\n                  <jats:p>N-glycosylation, a crucial post-translational modification, is well-recognized for its pivotal role in cardiovascular functions. N-acetylglucosaminyltransferase V (GnT-V) is one of the major glycosyltransferases that determine the complexity of N-glycans in N-glycosylation modification. This study aimed to explore the role of GnT-V in myocardial infarction (MI).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jtim-2025-0021_s_008\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Proteomics and N-glycoproteomic analysis were performed on myocardial tissues for the N-glycosylation profile after MI. Adeno-associated virus (AAV) with a mouse cTnT promoter was utilized to induce overexpression of GnT-V in the heart for the role of GnT-V in MI. Echocardiography and histological analysis were used to evaluate the effect of GnT-V on MI. For the potential mechanisms of GnT-V, proteomic analysis was performed on cardiomyocytes that were subjected to GnT-V overexpression and hypoxic stress. The results were validated by western blot, lectin blot and immunoprecipitation assays, and confirmed with PNGase F and tunicamycin treatment.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jtim-2025-0021_s_009\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>N-glycosylation of protein was significantly reduced after MI, which could be related to a decrease in the expression levels of GnT-V and its target glycans. Targeted GnT-V overexpression in the heart by using AAV improved cardiac function and reduced the infarct size after MI. Further, proteomics analysis of cardiomyocytes revealed that insulin-like growth factor-binding protein 3 (IGFBP3) was targeted by GnT-V and induced degradation through the lysosome pathway. Consequently, the insulin-like growth factor 1 receptor (IGF1R) signaling pathway was activated through overexpression of GnT-V.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"j_jtim-2025-0021_s_010\">\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Our findings suggest that promoting the IGF1R signaling cascades by regulating the N-glycosylation of certain proteins in the signaling pathway, especially through GnT-V, may act as a promising strategy for treating MI.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/jtim-2025-0021","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T13:02:16Z","timestamp":1750424536000},"page":"281-294","source":"Crossref","is-referenced-by-count":3,"title":["N-acetylglucosaminyltransferase V attenuates myocardial infarction by mediating the insulin-like growth factor 1 receptor signaling pathway"],"prefix":"10.1515","volume":"13","author":[{"given":"Tianqi","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital, State Key Laboratory of Vascular Homeostasis and Remodeling, NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Beijing Key Laboratory of Cardiovascular Receptors Research, Peking University , Beijing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangya'nan","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Chemistry and Molecular Engineering, Peking University , Beijing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenyu","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital, State Key Laboratory of Vascular Homeostasis and Remodeling, NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Beijing Key Laboratory of Cardiovascular Receptors Research, Peking University , Beijing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital, State Key Laboratory of Vascular Homeostasis and Remodeling, NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Beijing Key Laboratory of Cardiovascular Receptors Research, Peking University , Beijing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital, State Key Laboratory of Vascular Homeostasis and Remodeling, NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Beijing Key Laboratory of Cardiovascular Receptors Research, Peking University , Beijing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yidan","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital, State Key Laboratory of Vascular Homeostasis and Remodeling, NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Beijing Key Laboratory of Cardiovascular Receptors Research, Peking University , Beijing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Houhua","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Natural and Biomimetic Drugs, Chemical Biology Center, School of Pharmaceutical Sciences, Peking University , Beijing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7735-0955","authenticated-orcid":false,"given":"Jiaxing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital, State Key Laboratory of Vascular Homeostasis and Remodeling, NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptides, Beijing Key Laboratory of Cardiovascular Receptors Research, Peking University , Beijing , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1680-3628","authenticated-orcid":false,"given":"Ming","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cardiology and Institute of Vascular Medicine, Peking University Third Hospital, State Key Laboratory of Vascular Homeostasis and Remodeling, NHC Key Laboratory of Cardiovascular Molecular Biology and Regulatory 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