{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T22:09:08Z","timestamp":1774649348444,"version":"3.50.1"},"reference-count":19,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>\n                  <jats:bold>Introduction:<\/jats:bold>\n                  <jats:italic>Mycoplasma gallisepticum<\/jats:italic> is considered the most pathogenic and economically significant avian <jats:italic>Mycoplasma<\/jats:italic> spp. for the worldwide poultry industry. The aim of this study was to develop a novel and sensitive real-time loop-mediated isothermal amplification (LAMP) assay based on the amplification of its <jats:italic>mgc2<\/jats:italic> gene sequence for its rapid molecular detection in poultry.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Material and Methods:<\/jats:bold> Blood samples from 300 broiler and layer chickens were screened using a rapid serum agglutination (RSA) test. A real-time LAMP reaction was conducted with seropositive swab samples at 60\u00b0C for 90 min in an ESEQuant tube scanner using 6-carboxyfluorescein as the reporting dye.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Results:<\/jats:bold> The sensitivity of the developed assay was 10 fg\/\u03bcL of DNA. The assay was found 100% specific, showing no cross-reactivity with other avian <jats:italic>Mycoplasma<\/jats:italic> species. The proportion found of the positive samples by the real-time LAMP was 58%. In comparison, the RSA was found to detect 52% of positive cases.<\/jats:p>\n               <jats:p>\n                  <jats:bold>Conclusion:<\/jats:bold> The <jats:italic>mgc2<\/jats:italic> real-time LAMP emerged as a more sensitive and accurate method for molecular detection of <jats:italic>M. gallisepticum<\/jats:italic> than RSA. Robustness and precision give it applicability as a potential field diagnostic tool for <jats:italic>M. gallisepticum<\/jats:italic> control. The study will be beneficial in reducing economic losses that <jats:italic>M. gallisepticum<\/jats:italic> inflicts on the poultry industry. This is the first reported development of a real-time LAMP assay based on the amplification of the <jats:italic>mgc2<\/jats:italic> gene sequence using an ESEQuant tube scanner for galline <jats:italic>M. gallisepticum<\/jats:italic> detection.<\/jats:p>","DOI":"10.1515\/jvetres-2017-0058","type":"journal-article","created":{"date-parts":[[2017,11,30]],"date-time":"2017-11-30T22:17:03Z","timestamp":1512080223000},"page":"439-444","source":"Crossref","is-referenced-by-count":9,"title":["Real-time loop-mediated isothermal amplification (LAMP) of <i>mgc2<\/i> gene of <i>Mycoplasma gallisepticum<\/i>"],"prefix":"10.1515","volume":"61","author":[{"given":"Syed","family":"Ehtisham-ul-Haque","sequence":"first","affiliation":[{"name":"Department of Pathobiology , University of Veterinary and Animal Sciences , Lahore Sub-Campus Jhang - 35200 , Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madiha","family":"Kiran","sequence":"additional","affiliation":[{"name":"Department of Pathobiology , University of Veterinary and Animal Sciences , Lahore Sub-Campus Jhang - 35200 , Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Usman","family":"Waheed","sequence":"additional","affiliation":[{"name":"Department of Pathobiology , University of Veterinary and Animal Sciences , Lahore Sub-Campus Jhang - 35200 , Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Younus","sequence":"additional","affiliation":[{"name":"Department of Pathobiology , University of Veterinary and Animal Sciences , Lahore Sub-Campus Jhang - 35200 , Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2017,12,27]]},"reference":[{"key":"2021040703195307785_j_jvetres-2017-0058_ref_001_w2aab3b7b9b1b6b1ab1ab1Aa","doi-asserted-by":"crossref","unstructured":"1. 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