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This study is to explore the significance of let-7g and its novel target gene in bone metastasis of breast cancer.<\/jats:p><jats:p>The expression of let-7g or forkhead box C2 (FOXC2) was measured in human clinical breast cancer tissues with bone metastasis by using quantitative real-time Polymerase Chain Reaction (qRT-PCR). After transfection with let-7g or anti-let-7g in breast cancer cell linesMDA-MB-231or SK-BR3, qRT-PCR and Western blot were done to test the levels of let-7g and FOXC2. The effect of anti-let-7g and\/ or FOXC2 RNA interference (RNAi) on cell migration in breast cancer cells was evaluated by using wound healing assay.<\/jats:p><jats:p>Clinically, qRT-PCR showed that FOXC2 levels were higher in breast cancer tissues with bone metastasis than those in their noncancerous counterparts. Let-7g was showed to be negatively correlated with FOXC2 in human breast cancer samples with bone metastasis. We found that enforced expression of let-7g reduced levels of FOXC2 protein by using Western blot in MDA-MB-231 cells. Conversely, anti-let-7g enhanced levels of FOXC2 in SK-BR3 cells. In terms of function, anti-let-7g accelerated migration of SK-BR3 cells. Interestingly, FOXC2 RNAi abrogated anti-let-7g-mediated migration in breast cancer cells. Thus, we conclude that let-7g suppresses cell migration through targeting FOXC2 in breast cancer. Our finding provides a new perspective for understanding the mechanism of bone metastasis in breast cancer.<\/jats:p>","DOI":"10.1515\/med-2017-0023","type":"journal-article","created":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T10:01:34Z","timestamp":1504778494000},"page":"157-162","source":"Crossref","is-referenced-by-count":11,"title":["MicroRNA let-7g directly targets forkhead box C2 (FOXC2) to modulate bone metastasis in breast cancer"],"prefix":"10.1515","volume":"12","author":[{"given":"Lei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Orthopaedics , The First Affiliated Hospital of Xi\u2018an Medical University , Xi\u2019an 710077 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[{"name":"The Second Department of Geriatrics , Ninth Hospital of Xi\u2018an , Xi\u2019an 710054 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics , The First Affiliated Hospital of Xi\u2018an Medical University , Xi\u2019an 710077 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics , Ninth Hospital of Xi\u2018an , Xi\u2019an 710054 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2017,9,6]]},"reference":[{"key":"2022042708270256833_j_med-2017-0023_ref_001_w2aab2b8d228b1b7b1ab2b1b1Aa","doi-asserted-by":"crossref","unstructured":"Jemal A., Bray F., Center M.M., Ferlay J., Ward E., Forman D., Global cancer statistics, CA Cancer J Clin., 2011,61(2),69-90","DOI":"10.3322\/caac.20107"},{"key":"2022042708270256833_j_med-2017-0023_ref_002_w2aab2b8d228b1b7b1ab2b1b2Aa","doi-asserted-by":"crossref","unstructured":"Yates L.A., Norbury C.J., Gilbet R.J., The long and short of microRNA, Cell., 2013,153(3),516-519","DOI":"10.1016\/j.cell.2013.04.003"},{"key":"2022042708270256833_j_med-2017-0023_ref_003_w2aab2b8d228b1b7b1ab2b1b3Aa","doi-asserted-by":"crossref","unstructured":"Wei Y., Schober A., Weber C., Pathogenic arterial remodeling: the good and bad of microRNAs, Am J Physiol Heart Circ Physiol., 2013,304(8), H1050-1059","DOI":"10.1152\/ajpheart.00267.2012"},{"key":"2022042708270256833_j_med-2017-0023_ref_004_w2aab2b8d228b1b7b1ab2b1b4Aa","doi-asserted-by":"crossref","unstructured":"Wang Z., Yao H., Lin S., Zhu X., Shen Z., Lu G., et al., Transcriptional and epigenetic regulation of human microRNAs, Cancer Lett., 2013,331(1),1-10","DOI":"10.1016\/j.canlet.2012.12.006"},{"key":"2022042708270256833_j_med-2017-0023_ref_005_w2aab2b8d228b1b7b1ab2b1b5Aa","doi-asserted-by":"crossref","unstructured":"Pasquinelli A.E., Reinhart B.J., Slack F., Martindale M.Q., Kuroda M.I., Maller B., et al., Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA, Nature, 2000,408(6808),86-89","DOI":"10.1038\/35040556"},{"key":"2022042708270256833_j_med-2017-0023_ref_006_w2aab2b8d228b1b7b1ab2b1b6Aa","doi-asserted-by":"crossref","unstructured":"Bussing I., Slack F.J., Grosshans H., let-7 microRNAs in development, stem cells and cancer, Trends Mol Med. 2008,14(9),400-409","DOI":"10.1016\/j.molmed.2008.07.001"},{"key":"2022042708270256833_j_med-2017-0023_ref_007_w2aab2b8d228b1b7b1ab2b1b7Aa","doi-asserted-by":"crossref","unstructured":"Roush S., Slack F.J., The let-7 family of microRNAs, Trends Cell Biol., 2008,18(10),505-516","DOI":"10.1016\/j.tcb.2008.07.007"},{"key":"2022042708270256833_j_med-2017-0023_ref_008_w2aab2b8d228b1b7b1ab2b1b8Aa","doi-asserted-by":"crossref","unstructured":"Johnson S.M., Grosshans H., Shingara J., Byrom M., Jarvis R., Cheng A., et al., RAS is regulated by the let-7 microRNA family. 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