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Subsequently, AR mouse model was established to explore the role of miR-182-5p and TLR4 in AR <jats:italic>in vivo<\/jats:italic>. Initially, quantitative reverse transcription-PCR (qRT-PCR) analysis indicated that miR-182-5p was downregulated, while TLR4 expression was upregulated in AR mice. Then we found that miR-182-5p mimic reduced the frequency of sneezing and nose rubbing of the AR mice. In addition, miR-182-5p mimic significantly increased ovalbumin (OVA)-specific IgE and leukotriene C4 expression levels in nasal lavage fluid (NLF) and serum of AR mice. miR-182-5p mimic decreased the number of inflammatory cells in NLF of AR mice. It also reduced the levels of inflammatory factors in the serum of AR mice, such as interleukin (IL)-4, IL-5, IL-13, IL-17 and tumor necrosis factor (TNF)-\u03b1, while increasing the release of IFN-\u03b3 and IL-2. Finally, miR-182-5p mimic inhibited NF-\u03baB signaling pathway activation in AR mice. However, all effects of miR-182-5p mimic on AR mice were reversed by TLR4-plasmid. In conclusion, miR-182-5p\/TLR4 axis may represent a novel therapeutic target for AR.<\/jats:p>","DOI":"10.1515\/med-2020-0198","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T05:04:15Z","timestamp":1610514255000},"page":"1202-1212","source":"Crossref","is-referenced-by-count":9,"title":["MicroRNA-182-5p relieves murine allergic rhinitis via TLR4\/NF-\u03baB pathway"],"prefix":"10.1515","volume":"15","author":[{"given":"Aichun","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, The First Affiliated Hospital of Zhejiang Chinese Medical University , 54 Youdian Road, Shangcheng , Hangzhou , Zhejiang 310000 , People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yangzi","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, The First Affiliated Hospital of Zhejiang Chinese Medical University , 54 Youdian Road, Shangcheng , Hangzhou , Zhejiang 310000 , People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2020,12,24]]},"reference":[{"key":"2025041512135104199_j_med-2020-0198_ref_001_w2aab3b7d953b1b6b1ab2b1b1Aa","doi-asserted-by":"crossref","unstructured":"Settipane RA, Schwindt C. 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