{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T16:46:53Z","timestamp":1772902013143,"version":"3.50.1"},"reference-count":32,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background\/aims<\/jats:title>\n                  <jats:p>Uterine leiomyoma (ULM) is a kind of gene-involved benign tumor, which is located in the front of female reproductive tract. It is one of the most common reproductive tract tumors in women, which leads to abnormal menstruation, repeated pregnancy loss, and other serious gynecological diseases. Recently, microRNAs (miRNAs) have attracted much more attention in the process of exploring the molecular mechanisms of tumorigenesis. Furthermore, the deregulated miRNAs had been reported to play important roles in ULM pathology.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>In this study, we assessed the expression level of microRNA-199a-5p (miR-199a-5p) in human ULM by quantitative polymerase chain reaction. After that cell counting kit 8, colony formation, 5-ethynyl-20-deoxyuridine, flow cytometry, and Western blot analyses were performed to investigate the effects of miR-199a-5p on ULM cell proliferation and apoptosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We confirmed that miR-199a-5p was significantly downregulated in human ULM. The results of function analyses showed that miR-199a-5p inhibited cell proliferation and induced cell apoptosis <jats:italic>in vitro<\/jats:italic>. Bioinformatics tool showed oncogene <jats:italic>MED12<\/jats:italic> was one of the target genes of miR-199a-5p, which mediated the effect of miR-199a-5p on the ULM.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Our results showed that miR-199a-5p functioned as an antitumor factor in human ULM cells. These findings broaden the current findings on the function of miR-199a-5p into the ULM pathogenesis, and miR-199a-5p may serve as a prognosis and therapeutic target for the ULM and its related diseases.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1515\/med-2021-0348","type":"journal-article","created":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T12:53:13Z","timestamp":1641819193000},"page":"151-159","source":"Crossref","is-referenced-by-count":5,"title":["Effects of miRNA-199a-5p on cell proliferation and apoptosis of uterine leiomyoma by targeting MED12"],"prefix":"10.1515","volume":"17","author":[{"given":"Wei","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Women\u2019s Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital , Nanjing , Jiangsu 210004 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingyan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Zhangjiagang Hospital of Traditional Chinese Medicine and Affiliated Zhangjiagang Hospital of Nanjing University of Chinese Medicine , Zhangjiagang 215600 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Zhangjiagang Hospital of Traditional Chinese Medicine and Affiliated Zhangjiagang Hospital of Nanjing University of Chinese Medicine , Zhangjiagang 215600 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Zhangjiagang Hospital of Traditional Chinese Medicine and Affiliated Zhangjiagang Hospital of Nanjing University of Chinese Medicine , Zhangjiagang 215600 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumei","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, The Affiliated Huai\u2019an Hospital of Xuzhou Medical University and The Second People\u2019s Hospital of Huai\u2019an , No. 62, Huaihai Road (S.) , Huaian , Jiangsu 223002 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2022,1,10]]},"reference":[{"key":"2022120618373719506_j_med-2021-0348_ref_001","doi-asserted-by":"crossref","unstructured":"Zimmermann A, Bernuit D, Gerlinger C, Schaefers M, Geppert K. 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