{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T05:56:48Z","timestamp":1774677408848,"version":"3.50.1"},"reference-count":31,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2023,1,1]],"date-time":"2023-01-01T00:00:00Z","timestamp":1672531200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Hashimoto\u2019s thyroiditis (HT) is an autoimmune illness caused by a combination of genetic, epigenetic, and environmental factors. The pathogenesis of HT is not fully elucidated, especially in epigenetics. The epigenetic regulator Jumonji domain-containing protein D3 (JMJD3) has been extensively investigated in immunological disorders. This study has been performed to explore the roles and potential mechanisms of JMJD3 in HT. Thyroid samples from patients and healthy subjects were collected. We first analyzed the expression of JMJD3 and chemokines in the thyroid gland using real-time PCR and immunohistochemistry. <jats:italic>In vitro<\/jats:italic>, the apoptosis effect of the JMJD3-specific inhibitor GSK-J4 on the thyroid epithelial cell line Nthy-ori 3-1 was evaluated using FITC Annexin V Detection kit. Reverse transcription-polymerase chain reaction and Western blotting were applied to examine the inhibitory effect of GSK-J4 on the inflammation of thyrocytes. In the thyroid tissue of HT patients, JMJD3 messenger RNA  and protein levels were substantially greater than in controls (<jats:italic>P<\/jats:italic> &lt; 0.05). Chemokines C\u2013X\u2013C motif chemokine ligand 10 (CXCL10) and C\u2013C motif chemokine ligand 2 (CCL2) were elevated in HT patients, and thyroid cells with stimulation of tumor necrosis factor \u03b1 (TNF-\u03b1). GSK-J4 could suppress TNF-\u03b1-induced synthesis of chemokines CXCL10 and CCL2 and prohibit thyrocyte apoptosis. Our results shed light on the potential role of JMJD3 in HT and indicate that JMJD3 may become a novel therapeutic target in HT treatment and prevention.<\/jats:p>","DOI":"10.1515\/med-2023-0659","type":"journal-article","created":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T12:47:50Z","timestamp":1677674870000},"source":"Crossref","is-referenced-by-count":4,"title":["Role of Jumonji domain-containing protein D3 and its inhibitor GSK-J4 in Hashimoto\u2019s thyroiditis"],"prefix":"10.1515","volume":"18","author":[{"given":"Xixuan","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Cardiovascular and Cerebrovascular Disease Hospital, General Hospital of Ningxia Medical University , No. 804, Shengli South Street, Xingqing District , Yinchuan 750004 , Ningxia , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Radiology, The 942th Hospital of the People\u2019s Liberation Army Joint Logistics Support Force , Yinchuan , Ningxia , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Radiology, The 942th Hospital of the People\u2019s Liberation Army Joint Logistics Support Force , Yinchuan , Ningxia , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Cardiovascular and Cerebrovascular Disease Hospital, General Hospital of Ningxia Medical University , Yinchuan 750004 , Ningxia , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Cardiovascular and Cerebrovascular Disease Hospital, General Hospital of Ningxia Medical University , Yinchuan 750004 , Ningxia , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Cardiovascular and Cerebrovascular Disease Hospital, General Hospital of Ningxia Medical University , Yinchuan 750004 , Ningxia , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Sha","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Cardiovascular and Cerebrovascular Disease Hospital, General Hospital of Ningxia Medical University , Yinchuan 750004 , Ningxia , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2023,3,1]]},"reference":[{"key":"2023083109475946161_j_med-2023-0659_ref_001","doi-asserted-by":"crossref","unstructured":"Tozzoli R, Barzilai O, Ram M, Villalta D, Bizzaro N, Sherer Y, et al. Infections and autoimmune thyroid diseases: parallel detection of antibodies against pathogens with proteomic technology. Autoimmunity Rev. 2008;8(2):112\u20135.","DOI":"10.1016\/j.autrev.2008.07.013"},{"key":"2023083109475946161_j_med-2023-0659_ref_002","doi-asserted-by":"crossref","unstructured":"Hasham A, Tomer Y. Genetic and epigenetic mechanisms in thyroid autoimmunity. Immunol Res. 2012;54(1\u20133):204\u201313.","DOI":"10.1007\/s12026-012-8302-x"},{"key":"2023083109475946161_j_med-2023-0659_ref_003","doi-asserted-by":"crossref","unstructured":"Wiersinga WM. Thyroid autoimmunity. Endocr Dev. 2014;26:139\u201357.","DOI":"10.1159\/000363161"},{"key":"2023083109475946161_j_med-2023-0659_ref_004","doi-asserted-by":"crossref","unstructured":"Antonelli A, Ferrari SM, Giuggioli D, Ferrannini E, Ferri C, Fallahi P. Chemokine (C-X-C motif) ligand (CXCL)10 in autoimmune diseases. 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