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The mechanism to regulate inflammatory angiogenesis during tendon-bone healing remains to be elucidated. Here, we utilized lipopolysaccharide (LPS) to induce tenocyte inflammation and applied the conditioned medium from inflammatory tenocytes to treat rat aortic vascular endothelial cells (RAOECs). The results that indicated LPS treatment significantly induced the mRNA and protein upregulation of NLRP3, tumor necrosis factor \u03b1, IL-1\u03b2, and vascular endothelial growth factor A (VEGFA), as well as the secretion of VEGFA. Furthermore, the conditioned medium stimulated RAOEC angiogenesis. Celastrol, a quinone-methylated triterpenoid from\n                    <jats:italic>Tripterygium wilfordii<\/jats:italic>\n                    , has been reported to treat osteoarthritis. Here, celastrol could suppress LPS-induced upregulation of NLRP3 and IL-1\u03b2, and the secretion of VEGFA. Celastrol treatment also suppressed the conditioned medium-induced angiogenesis in RAOEC. Moreover, we established a rotator cuff tear rat model to stimulate tendon injury. Celastrol administration at the lesion significantly promoted tendon healing and functional recovery in injured mice by regulating NLRP3 and VEGFA levels. Taken together, these data suggest that the inflammation-induced tenocyte injury leads to angiogenesis, and that celastrol administration could suppress the inflammatory tenocyte-induced angiogenesis to promote tendon-bone healing via the NLRP3 pathway.\n                  <\/jats:p>","DOI":"10.1515\/med-2024-1121","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T11:00:41Z","timestamp":1747220441000},"source":"Crossref","is-referenced-by-count":2,"title":["Celastrol suppresses neovascularization in rat aortic vascular endothelial cells stimulated by inflammatory tenocytes via modulating the NLRP3 pathway"],"prefix":"10.1515","volume":"20","author":[{"given":"Yong","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Orthopedics, The Affiliated Shunde Hospital of Jinan University , Foshan , Guangdong Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huajun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, The First Affiliated Hospital, Guangdong Provincial Key Laboratory of Speed Capability, The Guangzhou Key Laboratory of Precision Orthopedics and Regenerative Medicine, Jinan University , 510630 , Guangzhou , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huige","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, The First Affiliated Hospital, Guangdong Provincial Key Laboratory of Speed Capability, The Guangzhou Key Laboratory of Precision Orthopedics and Regenerative Medicine, Jinan University , 510630 , Guangzhou , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiwen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The Affiliated Shunde Hospital of Jinan University , Foshan , Guangdong Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The Affiliated Shunde Hospital of Jinan University , Foshan , Guangdong Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjian","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The Affiliated Shunde Hospital of Jinan University , Foshan , Guangdong Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic, Hainan Hospital, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine , Guangzhou , China"},{"name":"Department of Orthopaedic, Hainan Traditional Chinese Medicine Hospital, Hainan Medical University , Hainan , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baofei","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic, Hainan Hospital, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine , Guangzhou , China"},{"name":"Department of Orthopaedic, Hainan Traditional Chinese Medicine Hospital, Hainan Medical University , Hainan , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhui","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, The Affiliated Shunde Hospital of Jinan University , Foshan , Guangdong Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, The First Affiliated Hospital, Guangdong Provincial Key Laboratory of Speed Capability, The Guangzhou Key Laboratory of Precision Orthopedics and Regenerative Medicine, Jinan University , 510630 , Guangzhou , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiguo","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic, Hainan Hospital, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine , Guangzhou , China"},{"name":"Department of Orthopaedic, Hainan Traditional Chinese Medicine Hospital, Hainan Medical University , Hainan , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyuan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine, The First Affiliated Hospital, Guangdong Provincial Key Laboratory of Speed Capability, The Guangzhou Key Laboratory of Precision Orthopedics and Regenerative Medicine, Jinan University , 510630 , Guangzhou , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,5,6]]},"reference":[{"key":"2025122201305034455_j_med-2024-1121_ref_001","doi-asserted-by":"crossref","unstructured":"Doiron-Cadrin P, Lafrance S, Saulnier M, Cournoyer E, Roy JS, Dyer JO, et al. Shoulder rotator cuff disorders: a systematic review of clinical practice guidelines and semantic analyses of recommendations. Arch Phys Med Rehabil. 2020;101(7):1233\u201342.","DOI":"10.1016\/j.apmr.2019.12.017"},{"key":"2025122201305034455_j_med-2024-1121_ref_002","doi-asserted-by":"crossref","unstructured":"De Rus Aznar I, Avila Lafuente JL, Hachem AI, Diaz Heredia J, Kany J, Elhassan B, et al. Tendon transfers for the management of irreparable subscapularis tears. Bone Jt J. 2024;106-B(9):970\u20137.","DOI":"10.1302\/0301-620X.106B9.BJJ-2024-0165.R1"},{"key":"2025122201305034455_j_med-2024-1121_ref_003","doi-asserted-by":"crossref","unstructured":"Linsell L, Dawson J, Zondervan K, Rose P, Randall T, Fitzpatrick R, et al. Prevalence and incidence of adults consulting for shoulder conditions in UK primary care; patterns of diagnosis and referral. 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