{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T01:46:26Z","timestamp":1766367986500,"version":"3.48.0"},"reference-count":26,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The specific role of lysophosphatidic acid 2 (LPA\n                      <jats:sub>2<\/jats:sub>\n                      ) in deep vein thrombosis (DVT) remains unclear.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      An inferior vena cava annulus retraction model of DVT was established in wild-type (WT) and global LPA\n                      <jats:sub>2<\/jats:sub>\n                      knockout (\n                      <jats:italic>Lpar2<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>\u2212\/\u2212<\/jats:italic>\n                      <\/jats:sup>\n                      ) mice. We examined the incidence of DVT, wet weight of thrombus, length of thrombus, assessed endothelial permeability through Evans blue dye assay\n                      <jats:italic>in vivo,<\/jats:italic>\n                      cell viability, and endothelial cell (EC) permeability of mouse inferior vena cava ECs\n                      <jats:italic>in vitro.<\/jats:italic>\n                      Proteomics, histopathology, immunohistochemistry, and western blotting were employed to investigate the role of LPA\n                      <jats:sub>2<\/jats:sub>\n                      in DVT.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Lpar2<\/jats:italic>\n                      deficiency increased vascular endothelial permeability and promoted the progression of DVT. Histological examination revealed aggravated inflammation in the thrombus of\n                      <jats:italic>Lpar2<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      DVT mice.\n                      <jats:italic>In vitro<\/jats:italic>\n                      ,\n                      <jats:italic>Lpar2<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      resulted in increased permeability of ECs. Proteomic results indicated that DVT after\n                      <jats:italic>Lpar2<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      may be related to tight junction (TJ) protein. LPA\n                      <jats:sub>2<\/jats:sub>\n                      agonist, 2-[4-(1,3-dioxo-1\n                      <jats:italic>H<\/jats:italic>\n                      ,3\n                      <jats:italic>H<\/jats:italic>\n                      -benzoisoquinolin-2-yl)butylsulfamoyl] benzoic acid, significantly reduced vascular endothelial permeability as well as increased expression of the vascular endothelial TJ protein zonula occludens-1.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These data provide a novel mechanism of endothelial barrier protection of LPA\n                      <jats:sub>2<\/jats:sub>\n                      in DVT.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/med-2024-1137","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T12:06:42Z","timestamp":1738843602000},"source":"Crossref","is-referenced-by-count":0,"title":["Lysophosphatidic acid 2 alleviates deep vein thrombosis via protective endothelial barrier function"],"prefix":"10.1515","volume":"20","author":[{"given":"Ruifeng","family":"Bai","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Jishuitan Hospital, Capital Medical University , Beijing , 100035 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyang","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Peking University Fourth School of Clinical Medicine , Beijing , 100035 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, Beijing Jishuitan Hospital, Capital Medical University , Beijing , 100035 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiru","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Jishuitan Hospital, Capital Medical University , Beijing , 100035 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[{"name":"Blood Transfusion Department, Beijing Jishuitan Hospital, Capital Medical University , Beijing , 100035 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Acute Abdomen Disease-Associated Organ Injury and ITCWM Repair, Institute of Integrative Medicine of Acute Abdominal Diseases, Tianjin Nankai Hospital, Tianjin Medical University , Tianjin 300100 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Beijing Jishuitan Hospital, Capital Medical University , 31 Xinjiekou East St. , Beijing , 100035 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2025,2,6]]},"reference":[{"key":"2025122201305466666_j_med-2024-1137_ref_001","doi-asserted-by":"crossref","unstructured":"Weitz JI, Chan NC. 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