{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T04:44:24Z","timestamp":1775882664300,"version":"3.50.1"},"reference-count":48,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_med-2026-1397_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Abnormal activation of the NLRP3 inflammasome is associated with various inflammatory diseases, making it a potential therapeutic target. A01 is an inhibitor of Smurf1, its effect on the activation of NLRP3 inflammasome is still unclear and needs further\u00a0study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_med-2026-1397_abs_002\">\n                    <jats:title>Material and methods<\/jats:title>\n                    <jats:p>Caspase-1 and IL-1\u03b2 were detected by immunoblotting and ELISA, and (lactate dehydrogenase) LDH release was measured via a specific kit. Immunoprecipitation was used to explore the interaction between NLRP3 and ASC. In a mouse model of alum-induced peritonitis, PECs were collected and analyzed by flow cytometry. In the high-fat diet (HFD) model, blood glucose was measured with a glucometer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_med-2026-1397_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A01 inhibited the activation of the NLRP3 inflammasome in macrophages without affecting the activation of AIM2 or NLRC4 inflammasomes. Mechanistically, A01 suppressed NLRP3 inflammasome assembly and activation by\u00a0disrupting the NLRP3-ASC interaction. Moreover, A01 demonstrated protective effects in mouse models of NLRP3\u00a0inflammasome-mediated diseases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_med-2026-1397_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      A01 specifically suppresses NLRP3 inflammasome activation\n                      <jats:italic>in\u00a0vitro<\/jats:italic>\n                      and\n                      <jats:italic>in\u00a0vivo<\/jats:italic>\n                      . A01 disrupts the association of NLRP3 and ASC. These findings suggest that A01 is a specific NLRP3 inhibitor and may be a promising candidate for treating NLRP3\u00a0inflammasome-related diseases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/med-2026-1397","type":"journal-article","created":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T03:48:31Z","timestamp":1775879311000},"source":"Crossref","is-referenced-by-count":0,"title":["Specific inhibition of NLRP3 inflammasome by a Smurf1 inhibitor\n                    <i>in\u00a0vitro<\/i>\n                    and\n                    <i>in\u00a0vivo<\/i>"],"prefix":"10.1515","volume":"21","author":[{"given":"Lifeng","family":"Meng","sequence":"first","affiliation":[{"name":"Department of Rheumatology , Zhuji People\u2019s Hospital of Zhejiang Province , Zhuji , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmei","family":"Xuan","sequence":"additional","affiliation":[{"name":"Department of Pain , Zhuji People\u2019s Hospital of Zhejiang Province , Zhuji , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Nephrology , Zhuji People\u2019s Hospital of Zhejiang Province , Zhuji , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weifeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Rheumatology , Zhuji People\u2019s Hospital of Zhejiang Province , Zhuji , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Bian","sequence":"additional","affiliation":[{"name":"Department of Nephrology , Zhuji People\u2019s Hospital of Zhejiang Province , Zhuji , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunxiao","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Nephrology , Zhuji People\u2019s Hospital of Zhejiang Province , Zhuji , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9075-6236","authenticated-orcid":false,"given":"Ying","family":"Guan","sequence":"additional","affiliation":[{"name":"Department of Rheumatology , Zhuji People\u2019s Hospital of Zhejiang Province , Zhuji , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2026,4,9]]},"reference":[{"key":"2026041103482695890_j_med-2026-1397_ref_001","doi-asserted-by":"crossref","unstructured":"Schroder, K, Tschopp, J. 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