{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T20:08:58Z","timestamp":1769371738006,"version":"3.49.0"},"reference-count":56,"publisher":"Walter de Gruyter GmbH","issue":"3","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p>In this study, we used PCR to detect and characterize <jats:italic>B. canis<\/jats:italic> from naturally infected dogs in Poland with subclinical babesiosis by amplifying and sequencing a portion of the 18S ribosomal RNA (rRNA) gene. Venous blood samples were collected from ten dogs with subclinical babesiosis. A 559-bp fragment of the <jats:italic>B. canis<\/jats:italic> 18S rRNA gene was amplified by PCR. Sequencing of the PCR products led to the identification of a new variant of <jats:italic>Babesia canis<\/jats:italic>, differing from the previously detected protozoa genotypes (18S rRNA-A and 18S rRNA-B) with nucleotide substitutions in positions 150 and 151 of the tested gene fragment. The results indicate the emergence within the Polish territory of a new, previously unencountered <jats:italic>Babesia canis<\/jats:italic> genotype responsible for the development of subclinical babesiosis.<\/jats:p>","DOI":"10.1515\/pjvs-2015-0074","type":"journal-article","created":{"date-parts":[[2015,11,30]],"date-time":"2015-11-30T17:01:41Z","timestamp":1448902901000},"page":"573-577","source":"Crossref","is-referenced-by-count":9,"title":["Identification of new 18S rRNA strains of Babesia canis isolated from dogs with subclinical babesiosis"],"prefix":"10.1515","volume":"18","author":[{"given":"P.","family":"\u0141yp","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0141.","family":"Adaszek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Furmaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Winiarczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref191","first-page":"1","article-title":"a Practical Approach st ed University","author":"McPherson","year":"1991"},{"key":"ref431","first-page":"109","article-title":"Schein The subspecies specificity ofBabesia canis","author":"Hauschild","year":"1996"},{"key":"ref201","first-page":"1067","article-title":"Hemostatic disorders during the course of canine babesiosis","author":"Milczak","year":"2004","journal-title":"Med Weter"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0304-4017(99)00049-7","article-title":"Molecular detection ofBabesia equiandBabesia caballiin horse blood by PCR amplification of part of the rRNA gene","volume":"16","author":"Bashiruddin","year":"1999","journal-title":"Vet Parasitol"},{"key":"ref151","first-page":"110","article-title":"Canine babesiosis in Romania due toBabesia canisandBabesia vogeli : a molecular approach","author":"Ionita","year":"2012","journal-title":"Parasitol Res"},{"key":"ref311","first-page":"106","article-title":"Application of the SYBR Green real - 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