{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T17:57:42Z","timestamp":1718042262126},"reference-count":44,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Animals are important reservoir of<jats:italic>Listeria monocytogenes<\/jats:italic>, a pathogen causing serious infections in both humans and livestock. However, data on invasiveness of<jats:italic>L. monocytogenes<\/jats:italic>strains of animal origin is very scarce. Ability of 18<jats:italic>L. monocytogenes<\/jats:italic>strains of animal origin to invade HT-29 cells was investigated. Plaque forming assay was used to assess invasiveness and ability of the pathogen to spread in the cell line. Almost 40% of<jats:italic>L. monocytogenes<\/jats:italic>strains were weakly invasive. It was shown that strains from serogroup 4b exhibited the highest invasiveness, whereas serogroup 1\/2b consisted of strains of invasiveness below 0.0001%. Analysis of translated<jats:italic>inlA<\/jats:italic>and<jats:italic>inlB<\/jats:italic>gene sequences revealed no premature stop codons. Lineage-specific mutations in low invasive strains were identified within<jats:italic>inlA<\/jats:italic>and<jats:italic>inlB<\/jats:italic>sequences. Our results demonstrate high incidence of low invasive animal<jats:italic>L. monocytogenes<\/jats:italic>strains, which may be at least partly explained by unique point mutations in the InlA and InlB.<\/jats:p>","DOI":"10.1515\/pjvs-2015-0090","type":"journal-article","created":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T19:50:56Z","timestamp":1453319456000},"page":"697-702","source":"Crossref","is-referenced-by-count":4,"title":["Invasiveness of Listeria monocytogenes strains isolated from animals in Poland"],"prefix":"10.1515","volume":"18","author":[{"given":"E.","family":"Wa\u0142ecka-Zacharska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Kosek-Paszkowska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Bania","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Staroniewicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Bednarski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Wieliczko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref151","first-page":"71","article-title":"Investigation of specific substitutions in virulence genes characterizing phenotypic groups of low - 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