{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T18:44:57Z","timestamp":1784141097747,"version":"3.55.0"},"reference-count":54,"publisher":"Walter de Gruyter GmbH","issue":"3","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objectives of this study were to determine the prevalence and antimicrobial resistance of coagulase-negative staphylococci (CNS) isolated from dairy cows with subclinical mastitis. Antimicrobial resistance in staphylococci were evaluated by breakpoint values specific to the species (EU-CAST). The presence of resistance-encoding genes was detected by multiplex PCR. A total of 191 CNS isolates were obtained. The CNS isolates were typically resistant to penicillin (67.4%), tetracyc-line (18.9%), and erythromycin (13.7%). CNS isolates (78.0%) were resistant to at least one antimicrobial compound, and 22.0% were multiresistant. The multiresistant isolates were predominantly<jats:italic>Staphylococcus chromogenes<\/jats:italic>(28.6%),<jats:italic>Staphylococcus warneri<\/jats:italic>(19%) and<jats:italic>Staphylococcus haemolyticus<\/jats:italic>(14.3%). According to MIC pattern data, multiresistant isolates showed the highest resistance (p&lt;0.05) rates to penicillin (85.7%), tetracycline (66.7%), and erythromycin (48.2%), but all of them were sensitive to daptomycin, oxacillin, qiunupristin\/dalfopristin, and vancomycin.<jats:italic>S. chrom<\/jats:italic>ogenes (9.5%),<jats:italic>S. haemolyticus<\/jats:italic>(4.8%), and<jats:italic>S. capitis ss capitis<\/jats:italic>(2.4%) strains were resistant to methicillin; their resistance to oxacillin and penicillin was more than 8 mg\/l. A high rate of resistance to penicillin was linked to a<jats:italic>bla<\/jats:italic>Z gene found in 66.6% of the isolated multiresistant CNS strains. Resistance to tetracycline via the<jats:italic>tet<\/jats:italic>K (38.1%) gene and penicillin via the<jats:italic>mec<\/jats:italic>A (23.8%) gene were detected less frequently. Gene<jats:italic>msr<\/jats:italic>AB was responsible for macrolides and lincosamides resistance and detected in 28.6% of the CNS isolates. Antimicrobial resistance genes were identified more frequently in<jats:italic>S. epidermidis<\/jats:italic>,<jats:italic>S. chromogenes<\/jats:italic>, and<jats:italic>S. warneri<\/jats:italic>.<\/jats:p>","DOI":"10.1515\/pjvs-2016-0080","type":"journal-article","created":{"date-parts":[[2016,10,19]],"date-time":"2016-10-19T10:02:47Z","timestamp":1476871367000},"page":"639-646","source":"Crossref","is-referenced-by-count":21,"title":["Phenotypical and Genotypical Antimicrobial Resistance of Coagulase-negative staphylococci Isolated from Cow Mastitis"],"prefix":"10.1515","volume":"19","author":[{"given":"I.","family":"Klimiene","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Virgailis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Pavilonis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Siugzdiniene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Mockeliunas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ruzauskas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"ref61","doi-asserted-by":"crossref","first-page":"2568","DOI":"10.1128\/JCM.00343-12","article-title":"Performances of the Vitek MS matrix - 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