{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T00:41:05Z","timestamp":1762044065569},"reference-count":48,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p>Studies identifying specific pathologically expressed genes have been performed on diseased myocardial tissue samples, however less invasive studies on gene expression of peripheral blood mononucleated cells give promising results. This study assessed transcriptomic data that may be used to evaluate Dachshunds with chronic mitral valve disease. Dachshunds with different stages of heart disease were compared to a control, healthy group. Microarray data analysis revealed clusters of patients with similar expression profiles. The clusters were compared to the clinical classification scheme. Unsupervised classification of the studied groups showed three clusters. Clinical and laboratory parameters of patients from the cluster 1 were in accordance with those found in patients without heart disease. Data obtained from patients from the cluster 3 were typical of advanced heart failure patients. Comparison of the cluster 1 and 3 groups revealed 1133 differentially expressed probes, 7 significantly regulated process pathways and 2 significantly regulated Ariadne Metabolic Pathways. This study may serve as a guideline for directing future research on gene expression in chronic mitral valve disease.<\/jats:p>","DOI":"10.1515\/pjvs-2016-0106","type":"journal-article","created":{"date-parts":[[2017,1,16]],"date-time":"2017-01-16T10:03:28Z","timestamp":1484561008000},"page":"849-857","source":"Crossref","is-referenced-by-count":2,"title":["Correlation between peripheral blood cell transcriptomic profile and clinical parameters of chronic mitral valve disease in Dachshunds"],"prefix":"10.1515","volume":"19","author":[{"given":"M.","family":"Garncarz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Hulanicka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Maciejewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Parzeniecka-Jaworska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Jank","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref151","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1939-1676.2010.0628.x","article-title":"The cardiac biomarker sodium - calcium exchanger can differentiate between heart failure and renal failure : a comparative study of NCX expression in dogs with chronic mitral valvular insufficiency and azotemia","volume":"24","author":"Nam","year":"2010","journal-title":"J Vet Intern Med"},{"key":"ref361","first-page":"1","article-title":"Identification of loci associated with development of myxomatous mitral valve disease in Cavalier Spaniels","author":"Madsen","year":"2011","journal-title":"J Hered"},{"key":"ref21","first-page":"183","article-title":"Histomorphological findings and expression of matrix metalloproteinases and their tissue specific inhibitors in normal tricuspid valves and in chronic tricuspid valvular disease in dogs","author":"Aupperle","year":"2010","journal-title":"Vet J"},{"key":"ref31","first-page":"402","article-title":"rd EC Interstitial cells from dogs with naturally occurring myxomatous mitral valve disease undergo phenotype transformation","volume":"17","author":"Disatian","year":"2008","journal-title":"J Heart Valve Dis"},{"key":"ref201","first-page":"99","article-title":"Real - time RT - PCR for gene expression profiling in blood of heart failure patients a pilot study : gene expression in blood of heart failure patients","author":"Seiler","year":"2004","journal-title":"Basic Res Cardiol"},{"key":"ref301","first-page":"193","article-title":"AT Pong KM wide analysis of mitral valve disease in Cavalier Spaniels","author":"French","year":"2012","journal-title":"Genome Vet J"},{"key":"ref231","first-page":"53","article-title":"blood cell transcriptome correlates with renal function in acute heart failure","author":"Szmit","year":"2012","journal-title":"Int Heart J"},{"key":"ref461","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2202\/1544-6115.1027","article-title":"Linear models and empirical Bayes methods for assessing differential expression in microarray experiments","volume":"3","author":"Smyth","year":"2004","journal-title":"Stat Appl Genet Mol Biol"},{"key":"ref481","first-page":"53","article-title":"blood cell transcriptome correlates with renal function in acute heart failure","author":"Szmit","year":"2012","journal-title":"Int Heart J"},{"key":"ref101","first-page":"188","article-title":"of premature mitral valve disease in Cavalier Spaniels","author":"Lewis","year":"2011","journal-title":"Vet J"},{"key":"ref351","first-page":"188","article-title":"of premature mitral valve disease in Cavalier Spaniels","author":"Lewis","year":"2011","journal-title":"Vet J"},{"key":"ref241","first-page":"119","article-title":"italia Microarray identifies extensive downregulation of noncollagen extracellular matrix and profibrotic growth factor genes in chronic isolated mitral regurgitation in the dog","author":"Zheng","year":"2009","journal-title":"Circulation"},{"key":"ref91","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1111\/j.1939-1676.2009.0332.x","article-title":"Calcium reuptake related genes as a cardiac biomarker in dogs with chronic mitral valvular insufficiency","volume":"23","author":"Lee","year":"2009","journal-title":"J Vet Intern Med"},{"key":"ref411","first-page":"72","article-title":"Effects of transforming growth factor - \u03b2 and matrix metalloproteinase - on the pathogenesis of chronic mitral valvular disease in dogs","author":"Obayashi","year":"2011","journal-title":"Am J Vet Res"},{"key":"ref281","first-page":"402","article-title":"rd EC Interstitial cells from dogs with naturally occurring myxomatous mitral valve disease undergo phenotype transformation","volume":"17","author":"Disatian","year":"2008","journal-title":"J Heart Valve Dis"},{"key":"ref381","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.molmed.2007.08.003","article-title":"The peripheral - blood transcriptome : new insights into disease and risk assessment","volume":"13","author":"Mohr","year":"2007","journal-title":"Trends Mol Med"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1186\/1471-2164-15-509","article-title":"The transcriptomic profile of peripheral blood nuclear cells in dogs with heart failure","volume":"15","author":"Hulanicka","year":"2014","journal-title":"BMC Genomics"},{"key":"ref421","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1111\/j.1939-1676.1999.tb01462.x","article-title":"HD inheritance of mitral valve prolapsed in Dachshunds","volume":"13","author":"Olsen","year":"1999","journal-title":"Epidemiology J Vet Intern Med"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1111\/j.1939-1676.2009.0392.x","article-title":"Guidelines for the Diagnosis and Treatment of Canine Chronic Valvular Disease","volume":"23","author":"Atkins","year":"2009","journal-title":"Heart J Vet Intern Med"},{"key":"ref51","first-page":"193","article-title":"AT Pong KM wide analysis of mitral valve disease in Cavalier Spaniels","author":"French","year":"2012","journal-title":"Genome Vet J"},{"key":"ref71","first-page":"2015","article-title":"Orchestrating high - throughput genomic analysis with Bioconductor","volume":"12","author":"Huber","journal-title":"Nat Methods"},{"key":"ref341","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1111\/j.1939-1676.2009.0332.x","article-title":"Calcium reuptake related genes as a cardiac biomarker in dogs with chronic mitral valvular insufficiency","volume":"23","author":"Lee","year":"2009","journal-title":"J Vet Intern Med"},{"key":"ref161","first-page":"72","article-title":"Effects of transforming growth factor - \u03b2 and matrix metalloproteinase - on the pathogenesis of chronic mitral valvular disease in dogs","author":"Obayashi","year":"2011","journal-title":"Am J Vet Res"},{"key":"ref181","article-title":"Genomic expression patterns of cardiac tissues from dogs with dilated cardiomyopathy","author":"Oyama MA","year":"2005","journal-title":"Am J Vet Res"},{"key":"ref01","first-page":"110","article-title":"Acquired Valvular Disease In LP eds Manual of Canine and Feline th ed St pp","author":"Abbott","year":"2008","journal-title":"Cardiology"},{"key":"ref221","first-page":"2015","article-title":"Severity of Mitral Valve Degeneration Is Associated with Loci in Whippet Dogs One","volume":"15","author":"Stern","journal-title":"Chromosome"},{"key":"ref331","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1186\/1471-2164-15-509","article-title":"The transcriptomic profile of peripheral blood nuclear cells in dogs with heart failure","volume":"15","author":"Hulanicka","year":"2014","journal-title":"BMC Genomics"},{"key":"ref371","first-page":"20","article-title":"Reduced sarcoplasmic reticulum Ca uptake and increased Na - Ca exchanger expression in left ventricle myocardium of dogs with progression of heart failure","author":"Mishra","year":"2005","journal-title":"Heart Vessels"},{"key":"ref321","first-page":"2015","article-title":"Orchestrating high - throughput genomic analysis with Bioconductor","volume":"12","author":"Huber","journal-title":"Nat Methods"},{"key":"ref171","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1111\/j.1939-1676.1999.tb01462.x","article-title":"HD inheritance of mitral valve prolapsed in Dachshunds","volume":"13","author":"Olsen","year":"1999","journal-title":"Epidemiology J Vet Intern Med"},{"key":"ref311","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1186\/1751-0147-55-52","article-title":"retrospective study of clinical signs and epidemiology of chronic valve disease in a group of Dachshund dogs in","volume":"55","author":"Garncarz","year":"2013","journal-title":"Acta Vet Scand"},{"key":"ref261","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1111\/j.1939-1676.2009.0392.x","article-title":"Guidelines for the Diagnosis and Treatment of Canine Chronic Valvular Disease","volume":"23","author":"Atkins","year":"2009","journal-title":"Heart J Vet Intern Med"},{"key":"ref191","first-page":"2015","article-title":"powers differential expression analyses for sequencing and microarray studies","author":"Ritchie","journal-title":"RNA Nucleic Acids Res"},{"key":"ref271","first-page":"183","article-title":"Histomorphological findings and expression of matrix metalloproteinases and their tissue specific inhibitors in normal tricuspid valves and in chronic tricuspid valvular disease in dogs","author":"Aupperle","year":"2010","journal-title":"Vet J"},{"key":"ref431","article-title":"Genomic expression patterns of cardiac tissues from dogs with dilated cardiomyopathy","author":"Oyama MA","year":"2005","journal-title":"Am J Vet Res"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1186\/1751-0147-55-52","article-title":"retrospective study of clinical signs and epidemiology of chronic valve disease in a group of Dachshund dogs in","volume":"55","author":"Garncarz","year":"2013","journal-title":"Acta Vet Scand"},{"key":"ref251","first-page":"110","article-title":"Acquired Valvular Disease In LP eds Manual of Canine and Feline th ed St pp","author":"Abbott","year":"2008","journal-title":"Cardiology"},{"key":"ref291","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1111\/j.1939-1676.2004.tb02575.x","article-title":"and Hematologic Variables in Dogs with Congestive Failure","volume":"18","author":"Farabaugh","year":"2004","journal-title":"Heart J Vet Intern Med"},{"key":"ref131","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.molmed.2007.08.003","article-title":"The peripheral - blood transcriptome : new insights into disease and risk assessment","volume":"13","author":"Mohr","year":"2007","journal-title":"Trends Mol Med"},{"key":"ref121","first-page":"20","article-title":"Reduced sarcoplasmic reticulum Ca uptake and increased Na - Ca exchanger expression in left ventricle myocardium of dogs with progression of heart failure","author":"Mishra","year":"2005","journal-title":"Heart Vessels"},{"key":"ref211","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2202\/1544-6115.1027","article-title":"Linear models and empirical Bayes methods for assessing differential expression in microarray experiments","volume":"3","author":"Smyth","year":"2004","journal-title":"Stat Appl Genet Mol Biol"},{"key":"ref491","first-page":"119","article-title":"italia Microarray identifies extensive downregulation of noncollagen extracellular matrix and profibrotic growth factor genes in chronic isolated mitral regurgitation in the dog","author":"Zheng","year":"2009","journal-title":"Circulation"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1111\/j.1939-1676.2004.tb02575.x","article-title":"and Hematologic Variables in Dogs with Congestive Failure","volume":"18","author":"Farabaugh","year":"2004","journal-title":"Heart J Vet Intern Med"},{"key":"ref451","first-page":"99","article-title":"Real - time RT - PCR for gene expression profiling in blood of heart failure patients a pilot study : gene expression in blood of heart failure patients","author":"Seiler","year":"2004","journal-title":"Basic Res Cardiol"},{"key":"ref471","first-page":"2015","article-title":"Severity of Mitral Valve Degeneration Is Associated with Loci in Whippet Dogs One","volume":"15","author":"Stern","journal-title":"Chromosome"},{"key":"ref111","first-page":"1","article-title":"Identification of loci associated with development of myxomatous mitral valve disease in Cavalier Spaniels","author":"Madsen","year":"2011","journal-title":"J Hered"},{"key":"ref401","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1939-1676.2010.0628.x","article-title":"The cardiac biomarker sodium - calcium exchanger can differentiate between heart failure and renal failure : a comparative study of NCX expression in dogs with chronic mitral valvular insufficiency and azotemia","volume":"24","author":"Nam","year":"2010","journal-title":"J Vet Intern Med"},{"key":"ref441","first-page":"2015","article-title":"powers differential expression analyses for sequencing and microarray studies","author":"Ritchie","journal-title":"RNA Nucleic Acids Res"}],"container-title":["Polish Journal of Veterinary Sciences"],"original-title":[],"link":[{"URL":"http:\/\/content.sciendo.com\/view\/journals\/pjvs\/19\/4\/article-p849.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.degruyter.com\/view\/j\/pjvs.2016.19.issue-4\/pjvs-2016-0106\/pjvs-2016-0106.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,7,22]],"date-time":"2019-07-22T15:51:39Z","timestamp":1563810699000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.pan.pl\/dlibra\/publication\/121026\/edition\/105427\/content"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,1]]},"references-count":48,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.1515\/pjvs-2016-0106","relation":{},"ISSN":["2300-2557"],"issn-type":[{"value":"2300-2557","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,1]]}}}