{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T03:26:29Z","timestamp":1777519589269,"version":"3.51.4"},"reference-count":68,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2017,3,28]],"date-time":"2017-03-28T00:00:00Z","timestamp":1490659200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,3,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p> Leptin modulates reproductive activity but its potential influence on LH secretion from anterior pituitary (AP) cells during implantation period in pigs (days 14-16 of pregnancy) remained unexplored. This study focused on determination whether leptin affects basal and GnRH-induced LH secretion and intracellular accumulation and whether leptin receptor (OB-Rb) mRNA is expressed in the AP gland during implantation in pigs. Four individual AP glands were developed into separate primary cultures. 2\u00d710<jats:sup>5<\/jats:sup> cells\/ml were preincubated (72 h) and next, for 3.5 h, experimentally treated with GnRH (100 ng\/ml), leptin (10<jats:sup>-11<\/jats:sup>, 10<jats:sup>-9<\/jats:sup>, 10<jats:sup>-7<\/jats:sup>, 10<jats:sup>-6<\/jats:sup> M) alone, or given in respective combinations with GnRH. In the AP gland, OB-Rb mRNA expression was determined by real-time PCR method. Leptin activated LH secretion and its concentration-dependent effect was observed as stimulation shown in a full range tested (culture 1) and exhibited only at 10<jats:sup>-6<\/jats:sup> M (culture 2). A pooled data analysis revealed that basal LH secretion increased at 10<jats:sup>-9<\/jats:sup>, 10<jats:sup>-7<\/jats:sup> and 10<jats:sup>-6<\/jats:sup> M, but GnRH-induced LH release decreased at 10<jats:sup>-6<\/jats:sup> M. Leptin down-regulated GnRH-induced LH secretion in all cultures, but only culture 3 exhibited sensitivity for all concentrations tested. Basal LH accumulation was activated in culture 1 (at 10<jats:sup>-11<\/jats:sup> M) and inhibited in culture 4 (at 10<jats:sup>-9<\/jats:sup> M). In the presence of GnRH leptin up-regulated LH accumulation with individual culture leptin-sensitivity (culture 1-3), while down-regulated LH accumulation in culture 4. Obtained data indicate that OB-Rb mRNA is expressed in the AP gland and leptin alone and in combination with GnRH specifically modulates LH activity during early pregnancy in pigs.<\/jats:p>","DOI":"10.1515\/pjvs-2017-0010","type":"journal-article","created":{"date-parts":[[2017,5,19]],"date-time":"2017-05-19T10:02:20Z","timestamp":1495188140000},"page":"67-76","source":"Crossref","is-referenced-by-count":4,"title":["In vitro effect of leptin on anterior pituitary cells LH secretory activity during early pregnancy in pig"],"prefix":"10.1515","volume":"20","author":[{"given":"G.","family":"Siawrys","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Gajewska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref411","article-title":"The GnRH receptor and the response of gonadotrope cells to GnRH pulse frequency code story of an atypical adaptation of cell function relying on a lack of receptor homologous desensitization","author":"Counis","year":"2009","journal-title":"Histochem"},{"key":"ref171","first-page":"48","article-title":"DA Influence of season and nutritional status on the direct effects of leptin orexin and ghrelin on luteinizing hormone and growth hormone secretion in the ovine pituitary explant model","author":"Kirsz","year":"2014"},{"key":"ref231","first-page":"144","article-title":"Effects of leptin on secretion of and FSH from primary cultured female rat pituitary cells","author":"Ogura","year":"2001","journal-title":"Eur J Endocrinol"},{"key":"ref371","first-page":"201","article-title":"Role of leptin in modulating the hypothalamic - pituitary axis and luteinizing hormone secretion in the prepuberal gilt","volume":"26","author":"Barb","year":"2004"},{"key":"ref511","first-page":"57","article-title":"and long form of leptin receptor genes expresssion in the hypothalamus and pituitary during the luteal phase and early pregnancy in pigs","author":"Kaminski","year":"2006","journal-title":"J Physiol Pharmacol"},{"key":"ref161","first-page":"57","article-title":"and long form of leptin receptor genes expresssion in the hypothalamus and pituitary during the luteal phase and early pregnancy in pigs","author":"Kaminski","year":"2006","journal-title":"J Physiol Pharmacol"},{"key":"ref361","first-page":"69","article-title":"acts at the bovine adenohypophysis to enhance basal and gonadotropin - releasing hormone - mediated release of luteinizing hormone : differential effects are dependent upon nutritional history","author":"Amstalden","year":"2003","journal-title":"Biol Reprod"},{"key":"ref561","first-page":"88","article-title":"Interactions of the GnRH receptor with heterotrimeric proteins Front","author":"Naor","year":"2013"},{"key":"ref241","first-page":"269","article-title":"Effects of the obese gene product on body weight regulation in ob \/ ob mice","author":"Pelleymounter","year":"1995","journal-title":"Science"},{"key":"ref491","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1095\/biolreprod63.5.1219","article-title":"in pregnancy","author":"Henson","year":"2000","journal-title":"Biol Reprod"},{"key":"ref41","first-page":"53","article-title":"The influences of and vasoactive intestinal peptide on PRL secretion by porcine pituitary cells in vitro","author":"Bogacka","year":"2002","journal-title":"oxytocin J Physiol Pharmacol"},{"key":"ref391","first-page":"53","article-title":"The influences of and vasoactive intestinal peptide on PRL secretion by porcine pituitary cells in vitro","author":"Bogacka","year":"2002","journal-title":"oxytocin J Physiol Pharmacol"},{"key":"ref471","first-page":"393","article-title":"Intracellular signalling pathways activated by leptin","author":"Fruhbeck","year":"2006","journal-title":"Biochem J"},{"key":"ref141","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1095\/biolreprod63.5.1219","article-title":"in pregnancy","author":"Henson","year":"2000","journal-title":"Biol Reprod"},{"key":"ref451","first-page":"29","article-title":"The control of adenohypophysial hormone secretion by amino acids and peptides in swine","author":"Estienne","year":"2005"},{"key":"ref601","first-page":"2015","article-title":"Role of leptin in female reproduction","volume":"53","author":"Perez","journal-title":"Clin Chem Lab Med"},{"key":"ref121","first-page":"393","article-title":"Intracellular signalling pathways activated by leptin","author":"Fruhbeck","year":"2006","journal-title":"Biochem J"},{"key":"ref341","first-page":"739","article-title":"Isolation and purification of porcine LH for radioimmunoassay and radioreceptor assay Pol","volume":"26","author":"Ziecik","year":"1979","journal-title":"Acad Sci Biol"},{"key":"ref481","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5625\/lar.2013.29.1.1","article-title":"Serum leptin concentrations leptin mRNA expression and food intake during the estrous cycle in rats","volume":"29","author":"Fungfuang","year":"2013","journal-title":"Lab Anim Res"},{"key":"ref501","first-page":"141","article-title":"Localization of leptin receptor - like immunoreactivity in the corticotropes somatotropes and gonadotropes in the ovine anterior pituitary","author":"Iqbal","year":"2000","journal-title":"Endocrinology"},{"key":"ref641","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.repbio.2012.10.003","article-title":"Direct in vitro effect of LH and steroids on leptin gene expression and leptin secretion by porcine luteal cells during the mid - luteal phase of the estrous cycle","volume":"12","author":"Siawrys","year":"2012","journal-title":"Reprod Biol"},{"key":"ref421","first-page":"145","article-title":"Apfelbaum Apfelbaum ME In vitro effect of leptin on LH release by anterior pituitary glands from female rats at the time of spontaneous and steroid - induced LH surge","author":"De Biasi","year":"2001","journal-title":"Eur J Endocrinol"},{"key":"ref401","first-page":"223","article-title":"FF years of leptin leptin and reproduction past milestones present undertakings and future endeavors","volume":"20","author":"Chehab","year":"2014","journal-title":"J Endocrinol"},{"key":"ref221","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/j.1740-0929.2006.00319.x","article-title":"Effects of leptin and leptin peptide amide on the release of luteinizing hormone growth hormone and prolactin from cultured porcine anterior pituitary cells","volume":"77","author":"Nonaka","year":"2006","journal-title":"Anim Sci J"},{"key":"ref11","first-page":"69","article-title":"acts at the bovine adenohypophysis to enhance basal and gonadotropin - releasing hormone - mediated release of luteinizing hormone : differential effects are dependent upon nutritional history","author":"Amstalden","year":"2003","journal-title":"Biol Reprod"},{"key":"ref101","first-page":"29","article-title":"The control of adenohypophysial hormone secretion by amino acids and peptides in swine","author":"Estienne","year":"2005"},{"key":"ref61","article-title":"The GnRH receptor and the response of gonadotrope cells to GnRH pulse frequency code story of an atypical adaptation of cell function relying on a lack of receptor homologous desensitization","author":"Counis","year":"2009","journal-title":"Histochem"},{"key":"ref301","first-page":"305","article-title":"Expression and localization of leptin receptor in the normal rat pituitary gland","author":"Sone","year":"2001","journal-title":"Cell Tissue Res"},{"key":"ref31","first-page":"479","article-title":"The expression of short form of leptin receptor gene during early pregnancy in the pig examined by quantitative real time RT","author":"Bogacka","year":"2006","journal-title":"J Physiol Pharmacol"},{"key":"ref81","first-page":"198","article-title":"modulates the expression of its receptors in the hypothalamic - pituitary - ovarian axis in a differential way","author":"Di Yorio","year":"2008","journal-title":"J Endocrinol"},{"key":"ref261","first-page":"89","article-title":"Effects of galanin and leptin on gonadotropin - releasing hormone - stimulated luteinizing hormone release from the pituitary","author":"Peters","year":"2009","journal-title":"Neuroendocrinology"},{"key":"ref271","first-page":"845","article-title":"Expression of leptin and long form of leptin receptor genes and proteins in pituitary of cyclic and pregnant pigs","author":"Siawrys","year":"2007","journal-title":"J Physiol Pharmacol"},{"key":"ref351","first-page":"210","article-title":"Serum leptin levels and reproductive function during the menstrual cycle","author":"Ahrens","year":"2014","journal-title":"Am J Obstet Gynecol"},{"key":"ref581","first-page":"144","article-title":"Effects of leptin on secretion of and FSH from primary cultured female rat pituitary cells","author":"Ogura","year":"2001","journal-title":"Eur J Endocrinol"},{"key":"ref51","first-page":"223","article-title":"FF years of leptin leptin and reproduction past milestones present undertakings and future endeavors","volume":"20","author":"Chehab","year":"2014","journal-title":"J Endocrinol"},{"key":"ref431","first-page":"198","article-title":"modulates the expression of its receptors in the hypothalamic - pituitary - ovarian axis in a differential way","author":"Di Yorio","year":"2008","journal-title":"J Endocrinol"},{"key":"ref01","first-page":"210","article-title":"Serum leptin levels and reproductive function during the menstrual cycle","author":"Ahrens","year":"2014","journal-title":"Am J Obstet Gynecol"},{"key":"ref541","first-page":"19","article-title":"GB Long form leptin receptor mRNA expression in the brain pituitary and other tissues in the pig","author":"Lin","year":"2000"},{"key":"ref691","first-page":"739","article-title":"Isolation and purification of porcine LH for radioimmunoassay and radioreceptor assay Pol","volume":"26","author":"Ziecik","year":"1979","journal-title":"Acad Sci Biol"},{"key":"ref91","first-page":"75","article-title":"Serum and milk concentrations of leptin in gilts fed a high - or low - energy diet during gestation","author":"Estienne","year":"2003","journal-title":"Anim Reprod Sci"},{"key":"ref151","first-page":"141","article-title":"Localization of leptin receptor - like immunoreactivity in the corticotropes somatotropes and gonadotropes in the ovine anterior pituitary","author":"Iqbal","year":"2000","journal-title":"Endocrinology"},{"key":"ref441","first-page":"75","article-title":"Serum and milk concentrations of leptin in gilts fed a high - or low - energy diet during gestation","author":"Estienne","year":"2003","journal-title":"Anim Reprod Sci"},{"key":"ref311","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1530\/jrf.0.0980643","article-title":"JE Prolactin as a luteotrophin during late pregnancy in pigs","author":"Szafranska","year":"1993","journal-title":"J Reprod Fertil"},{"key":"ref531","first-page":"439","article-title":"Effect of leptin insulin and on GnRH - induced LH secretion from porcine pituitary cells in vitro Vet","author":"Kosior","year":"2007","journal-title":"Inst"},{"key":"ref21","first-page":"201","article-title":"Role of leptin in modulating the hypothalamic - pituitary axis and luteinizing hormone secretion in the prepuberal gilt","volume":"26","author":"Barb","year":"2004"},{"key":"ref651","first-page":"305","article-title":"Expression and localization of leptin receptor in the normal rat pituitary gland","author":"Sone","year":"2001","journal-title":"Cell Tissue Res"},{"key":"ref551","first-page":"31390","article-title":"Nu rnberg Intrauterine growth retarded progeny of pregnant sows fed high protein : low carbohydrate diet is related to metabolic energy deficit One","author":"Metges","year":"2012"},{"key":"ref131","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5625\/lar.2013.29.1.1","article-title":"Serum leptin concentrations leptin mRNA expression and food intake during the estrous cycle in rats","volume":"29","author":"Fungfuang","year":"2013","journal-title":"Lab Anim Res"},{"key":"ref181","first-page":"439","article-title":"Effect of leptin insulin and on GnRH - induced LH secretion from porcine pituitary cells in vitro Vet","author":"Kosior","year":"2007","journal-title":"Inst"},{"key":"ref681","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1073\/pnas.94.3.1023","article-title":"Role of leptin in hypothalamic - pituitary function","author":"Yu","year":"1997","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref521","first-page":"48","article-title":"DA Influence of season and nutritional status on the direct effects of leptin orexin and ghrelin on luteinizing hormone and growth hormone secretion in the ovine pituitary explant model","author":"Kirsz","year":"2014"},{"key":"ref251","first-page":"2015","article-title":"Role of leptin in female reproduction","volume":"53","author":"Perez","journal-title":"Clin Chem Lab Med"},{"key":"ref671","first-page":"146","article-title":"Effects of leptin on gonadotropin secretion in juvenile female rat pituitary cells","author":"Tezuka","year":"2002","journal-title":"Eur J Endocrinol"},{"key":"ref331","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1073\/pnas.94.3.1023","article-title":"Role of leptin in hypothalamic - pituitary function","author":"Yu","year":"1997","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref381","first-page":"479","article-title":"The expression of short form of leptin receptor gene during early pregnancy in the pig examined by quantitative real time RT","author":"Bogacka","year":"2006","journal-title":"J Physiol Pharmacol"},{"key":"ref661","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1530\/jrf.0.0980643","article-title":"JE Prolactin as a luteotrophin during late pregnancy in pigs","author":"Szafranska","year":"1993","journal-title":"J Reprod Fertil"},{"key":"ref201","first-page":"31390","article-title":"Nu rnberg Intrauterine growth retarded progeny of pregnant sows fed high protein : low carbohydrate diet is related to metabolic energy deficit One","author":"Metges","year":"2012"},{"key":"ref631","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1111\/j.1439-0531.2008.01118.x","article-title":"Expression of leptin and long - form leptin - receptor proteins in porcine hypothalamus during oestrous cycle and pregnancy","author":"Siawrys","year":"2009","journal-title":"Reprod Domest Anim"},{"key":"ref191","first-page":"19","article-title":"GB Long form leptin receptor mRNA expression in the brain pituitary and other tissues in the pig","author":"Lin","year":"2000"},{"key":"ref571","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/j.1740-0929.2006.00319.x","article-title":"Effects of leptin and leptin peptide amide on the release of luteinizing hormone growth hormone and prolactin from cultured porcine anterior pituitary cells","volume":"77","author":"Nonaka","year":"2006","journal-title":"Anim Sci J"},{"key":"ref211","first-page":"88","article-title":"Interactions of the GnRH receptor with heterotrimeric proteins Front","author":"Naor","year":"2013"},{"key":"ref621","first-page":"845","article-title":"Expression of leptin and long form of leptin receptor genes and proteins in pituitary of cyclic and pregnant pigs","author":"Siawrys","year":"2007","journal-title":"J Physiol Pharmacol"},{"key":"ref71","first-page":"145","article-title":"Apfelbaum Apfelbaum ME In vitro effect of leptin on LH release by anterior pituitary glands from female rats at the time of spontaneous and steroid - induced LH surge","author":"De Biasi","year":"2001","journal-title":"Eur J Endocrinol"},{"key":"ref281","doi-asserted-by":"crossref","first-page":"920","DOI":"10.1111\/j.1439-0531.2008.01118.x","article-title":"Expression of leptin and long - form leptin - receptor proteins in porcine hypothalamus during oestrous cycle and pregnancy","author":"Siawrys","year":"2009","journal-title":"Reprod Domest Anim"},{"key":"ref321","first-page":"146","article-title":"Effects of leptin on gonadotropin secretion in juvenile female rat pituitary cells","author":"Tezuka","year":"2002","journal-title":"Eur J Endocrinol"},{"key":"ref611","first-page":"89","article-title":"Effects of galanin and leptin on gonadotropin - releasing hormone - stimulated luteinizing hormone release from the pituitary","author":"Peters","year":"2009","journal-title":"Neuroendocrinology"},{"key":"ref291","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.repbio.2012.10.003","article-title":"Direct in vitro effect of LH and steroids on leptin gene expression and leptin secretion by porcine luteal cells during the mid - luteal phase of the estrous cycle","volume":"12","author":"Siawrys","year":"2012","journal-title":"Reprod Biol"},{"key":"ref591","first-page":"269","article-title":"Effects of the obese gene product on body weight regulation in ob \/ ob mice","author":"Pelleymounter","year":"1995","journal-title":"Science"}],"container-title":["Polish Journal of Veterinary Sciences"],"original-title":[],"link":[{"URL":"http:\/\/content.sciendo.com\/view\/journals\/pjvs\/20\/1\/article-p67.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.degruyter.com\/view\/j\/pjvs.2017.20.issue-1\/pjvs-2017-0010\/pjvs-2017-0010.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,7,22]],"date-time":"2019-07-22T15:51:46Z","timestamp":1563810706000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.pan.pl\/dlibra\/publication\/121063\/edition\/105464\/content"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,28]]},"references-count":68,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.1515\/pjvs-2017-0010","relation":{},"ISSN":["2300-2557"],"issn-type":[{"value":"2300-2557","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,28]]}}}