{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T21:08:31Z","timestamp":1758402511720},"reference-count":56,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n<jats:p><jats:italic>Streptococcus suis<\/jats:italic> serotype 2 (SS2) is considered as a major pathogen that causes sepsis and meningitis in piglets and humans, but knowledge of its antigenic proteins remains limited so far. The surface-related proteins of pathogens often play significant roles in bacterium-host interactions and infection. Here, we obtained the elongation factor Tu (EF-Tu) gene of <jats:italic>Streptococcus suis<\/jats:italic> and constructed the recombinant expression plasmid successfully. The target recombinant plasmid was then expressed in <jats:italic>Escherichia coli<\/jats:italic> and the immuno-protection of the recombinant protein was subsequently evaluated as well. The EF-Tu gene of <jats:italic>Streptococcus suis<\/jats:italic> is 1197 bp in length, encodes 398 amino acids. The target recombinant EF-Tu (rEF-Tu) protein can recognize the antiserum of <jats:italic>Streptococcus suis<\/jats:italic> and can provoke obvious humoral immune responses in rabbits and conferred protection to rabbits against <jats:italic>Streptococcus suis<\/jats:italic> ear-vein challenge, implying that the EF-Tu may be used as an attractive candidate antigen for a component of subunit vaccine.<\/jats:p>","DOI":"10.1515\/pjvs-2017-0033","type":"journal-article","created":{"date-parts":[[2017,9,2]],"date-time":"2017-09-02T10:05:35Z","timestamp":1504346735000},"page":"277-284","source":"Crossref","is-referenced-by-count":4,"title":["Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2"],"prefix":"10.1515","volume":"20","author":[{"given":"X.J.","family":"Xia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.K.","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.Q.","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.G.","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.L.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref501","first-page":"617","article-title":"Wertheim Streptococcus suis an emerging human pathogen","author":"Nghia","year":"2009","journal-title":"Clin Infect Dis"},{"key":"ref491","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1046\/j.1439-0450.2001.00460.x","article-title":"de Andr\u00e9s Epidemiological relationship of human and swineStreptococcus suisisolates","author":"Tarradas","year":"2001","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"ref421","first-page":"270","article-title":"The immune enhancement of propolis adjuvant on inactivated porcine parvovirus vaccine in guinea pig","author":"Ma","year":"2011","journal-title":"Cell Immunol"},{"key":"ref451","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1038\/nbt1179","article-title":"Characterization and identification of vaccine candidate proteins through analysis of the group AStreptococcussurface proteome","volume":"24","author":"Rodr\u00edguez","year":"2006","journal-title":"Nat Biotechnol"},{"key":"ref271","first-page":"9801","article-title":"Immunoproteomic assay of membrane - associated proteins ofStreptococcus suistype China vaccine strain","author":"Zhang","year":"2007","journal-title":"Public Health"},{"key":"ref261","doi-asserted-by":"crossref","first-page":"4468","DOI":"10.1002\/pmic.200700294","article-title":"a Immunoproteomics of extracellular proteins of Chinese virulent strains ofStreptococcus suistype","author":"Zhang","year":"2007","journal-title":"Proteomics"},{"key":"ref301","first-page":"89","article-title":"Dilemma of virulence ofStreptococcus suis isolate characterized as a virulent strain using a standardized experimental model in pigs","author":"Berthelot","year":"2005","journal-title":"Canadian Can J Vet Res"},{"key":"ref371","first-page":"849","article-title":"Bacterial elongation factors their mutants chimeric forms and domains isolation purification","author":"Jonak","year":"2007","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"},{"key":"ref471","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1023\/A:1005870317757","article-title":"Streptococcus suis past present","volume":"21","author":"Staats","year":"1997","journal-title":"Vet Res Commun"},{"key":"ref521","first-page":"150","article-title":"Preliminary study on immunoproteomics ofMycoplasma ovipneumoniae Chinese Vet","author":"Xu","year":"2012","journal-title":"Sci"},{"key":"ref161","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1038\/nbt1179","article-title":"Characterization and identification of vaccine candidate proteins through analysis of the group AStreptococcussurface proteome","volume":"24","author":"Rodr\u00edguez","year":"2006","journal-title":"Nat Biotechnol"},{"key":"ref381","first-page":"39","article-title":"Streptococcus suisinfection in","volume":"88","author":"Kay","year":"1995"},{"key":"ref151","first-page":"1","article-title":"Expression and Western Blotting Analysis of Streptococcus suis Sao suis Chinese of Veterinary","volume":"50","author":"Yang","year":"2016","journal-title":"Journal Drug"},{"key":"ref221","first-page":"932","article-title":"of PPA - ELISA for the detection of antibodies againstStreptococcus suisusing recombinant GDH protein as antigen Chinese Vet","author":"Xia","year":"2012","journal-title":"Development Sci"},{"key":"ref201","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1046\/j.1439-0450.2001.00460.x","article-title":"de Andr\u00e9s Epidemiological relationship of human and swineStreptococcus suisisolates","author":"Tarradas","year":"2001","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"ref31","first-page":"2015","article-title":"MT Bacterial backstabbing brute","author":"Cabeen","journal-title":"Cell"},{"key":"ref411","first-page":"14361","article-title":"zu Responses to Bacterial Elongation Factor Tu duringBurkholderiaInfection and Immunization One","author":"Nieves","year":"2010"},{"key":"ref191","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1371\/journal.pmed.0030151","article-title":"Streptococcal toxic shock syndrome caused byStreptococcus suisserotype","author":"Tang","year":"2006","journal-title":"PLoS Med"},{"key":"ref231","first-page":"150","article-title":"Preliminary study on immunoproteomics ofMycoplasma ovipneumoniae Chinese Vet","author":"Xu","year":"2012","journal-title":"Sci"},{"key":"ref121","first-page":"14361","article-title":"zu Responses to Bacterial Elongation Factor Tu duringBurkholderiaInfection and Immunization One","author":"Nieves","year":"2010"},{"key":"ref41","first-page":"477","article-title":"Streptococcus suisinfection an emerging reemerging challenge of bacterial infectious diseases Virulence","author":"Feng","year":"2014"},{"key":"ref181","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1023\/A:1005870317757","article-title":"Streptococcus suis past present","volume":"21","author":"Staats","year":"1997","journal-title":"Vet Res Commun"},{"key":"ref391","first-page":"2015","article-title":"Identification of Novel and Fibronectin - 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