{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T04:27:42Z","timestamp":1778732862476,"version":"3.51.4"},"reference-count":68,"publisher":"Walter de Gruyter GmbH","issue":"3","license":[{"start":{"date-parts":[[2017,9,26]],"date-time":"2017-09-26T00:00:00Z","timestamp":1506384000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,9,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p>The aim of the study carried out on ten young (10-week old) pigs of the native Polish Large White breed experimentally infected with a low dose of 300 invasive muscle larvae (ML) of Trichinella spiralis was intravital detection of trichinellosis using the E-S ELISA test, determination of a variation level of IgG antibodies against excretory-secretory (E-S) antigens of T. spiralis muscle larvae and finally, describing the intensity of T. spiralis larvae infection in selected muscles. The pig sera were collected at 7 and 9 days prior to the experimental infection with T. spiralis and at 9, 14, 20, 23, 25, 27, 30, 33, 37, 41, 46 days post-infection (d.p.i.). The anti-T. spiralis IgG antibodies were detected by a commercial E-S ELISA test (PrioCHECK Trichinella Ab). Average intensity of the T. spiralis infection in the examined muscles of pigs ranged from 1.52 up to 43.09 larvae\/g. The studies revealed that the E-S antigen in the ELISA test did not show cross-reaction with the sera of pigs infected with Oesophagostomum spp. The ELISA assay did not recognize trichinellosis in pigs until 27 days after the T. spiralis infection. The anti-T. spiralis IgG antibodies were first detected on day 30 post-infection. A statistically significant increase of IgG antibodies against T. spiralis ML E-S antigens was first observed between days 27-30 (p&lt;0.01) post-infection, and a further significant rise in the antibody level occurred between days 27 and 33 (p&lt;0.01); 30 and 33 (p&lt;0.01); 33 and 37 (p&lt;0.05) following infection.<\/jats:p>","DOI":"10.1515\/pjvs-2017-0054","type":"journal-article","created":{"date-parts":[[2017,11,22]],"date-time":"2017-11-22T22:16:29Z","timestamp":1511388989000},"page":"445-454","source":"Crossref","is-referenced-by-count":5,"title":["Detection of Experimental Swine Trichinellosis Using Commercial Elisa Test"],"prefix":"10.1515","volume":"20","author":[{"given":"Michal","family":"Gondek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Bie\u0144","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Nowakowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref201","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/S0304-4017(00)00350-2","article-title":"Detection of Trichinella infection in food animals","author":"N\u00f6ckler","year":"2000","journal-title":"Vet Parasitol"},{"key":"ref631","first-page":"1","article-title":"Comparison of Trichinella spiralis larva antigens for the detection of specific antibodies in pigs Vet -","volume":"44","author":"Reiterov\u00e1","year":"1999","journal-title":"Med Czech"},{"key":"ref121","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1128\/CMR.00026-08","article-title":"No ckler diagnosis treatment and control of trichinellosis","volume":"22","author":"Gottstein","year":"2009","journal-title":"Epidemiology Clin Microbiol Rev"},{"key":"ref571","first-page":"108","article-title":"Diagnosis of trichinellosis in living pigs using indirect ELISA","author":"N\u00f6ckler","year":"1995","journal-title":"Berl Munch Tierarztl"},{"key":"ref261","first-page":"39","article-title":"Hosts and habitats of Trichinella spiralis and Trichinella britovi in Europe","author":"Pozio","year":"2009","journal-title":"Int J Parasitol"},{"key":"ref621","first-page":"593","article-title":"Prost of Trichinella spiralis in muscles by pooled - 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