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Assay sensitivity was 2.373 \u00d7 10<jats:sup>3<\/jats:sup> copies of the Ma5 strain, and 3.852 x 10<jats:sup>3<\/jats:sup> copies of the 4\/91 strain. Samples belonging to a known genotype demonstrated that the designed assays supported rapid and sensitive detection of Ma<jats:sup>5<\/jats:sup> and 4\/91 vaccine strains of IBV.<\/jats:p>","DOI":"10.1515\/pjvs-2017-0073","type":"journal-article","created":{"date-parts":[[2017,11,22]],"date-time":"2017-11-22T17:16:29Z","timestamp":1511370989000},"page":"599-601","source":"Crossref","is-referenced-by-count":7,"title":["Differentiation of infectious bronchitis virus vaccine strains Ma5 and 4\/91 by TaqMan real-time PCR"],"prefix":"10.1515","volume":"20","author":[{"given":"T.","family":"Stenzel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Dziewulska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"\u015amia\u0142ek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Tyka\u0142owski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Kowalczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Koncicki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref171","first-page":"223","article-title":"de Wit van der Heijden HM Infectious bronchitis virus variants a review of the current situation and control measures","author":"Sjaak","year":"2011","journal-title":"history Avian Pathol"},{"key":"ref181","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA molecular evolutionary genetics analysis using maximum likelihood evolutionary distance and maximum parsimony methods","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol Biol Evol"},{"key":"ref191","first-page":"194","article-title":"IN The avian coronavirus spike protein","author":"Wickramasinghe","year":"2014","journal-title":"Virus Res"},{"key":"ref111","first-page":"117","article-title":"Infectious bronchitis In eds Diseases of Poultry th ed Publishing Professional","volume":"12","author":"Cavanagh","year":"2008","journal-title":"Iowa USA"},{"key":"ref21","first-page":"1","article-title":"Location of the amino acid differences in the spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus","author":"Cavanagh","year":"1992","journal-title":"Avian Pathol"},{"key":"ref71","first-page":"223","article-title":"de Wit van der Heijden HM Infectious bronchitis virus variants a review of the current situation and control measures","author":"Sjaak","year":"2011","journal-title":"history Avian Pathol"},{"key":"ref61","first-page":"163","article-title":"of a real - 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