{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:24:52Z","timestamp":1790720692189,"version":"4.1.0"},"reference-count":36,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T00:00:00Z","timestamp":1748390400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2020-05760"],"award-info":[{"award-number":["RGPIN-2020-05760"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,7,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Notch1 is a mechanosensitive endothelial receptor that responds to vascular wall shear stress. However, data on Notch1 activity in humans remains limited, particularly regarding its role in endothelial function.<\/jats:p>\n                  <jats:sec id=\"j_teb-2025-0006_abs_001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Accordingly, the purpose of this study was to determine whether Notch1 is associated with the flow-mediated dilatory (FMD) response and whether it is related to aerobic fitness. A secondary purpose was to determine whether Notch1 is related to concentrations of vascular endothelial growth factor (VEGF).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_teb-2025-0006_abs_002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Sixteen (8M\/8F) young (20\u201330 yrs old) and healthy (BMI: 25\u00a0\u00b1\u00a04.2\u202fkg\/m\n                      <jats:sup>2<\/jats:sup>\n                      , blood pressure: 117\u00a0\u00b1\u00a011.63\/69\u00a0\u00b1 11.25\u202fmmHg) adults participated in the study. Aerobic fitness was determined by cycle V\u0307O\n                      <jats:sub>2<\/jats:sub>\n                      peak. An FMD was performed on the brachial artery, and blood samples were taken from an antecubital vein at rest (baseline) and 1\u202fmin after cuff deflation (to align with peak vessel dilation). Concentrations of Notch1 extracellular domain (NECD) and VEGF were determined from plasma using enzyme-linked immunosorbent assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_teb-2025-0006_abs_003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In contrast to our hypothesis, concentrations of NECD and VEGF did not change throughout the FMD and were unrelated to allometrically scaled FMD values (p all&gt;0.05). Likewise, there was no relationship between changes in NECD and VEGF (p=0.331, r=0.127). However, the change in NECD across the FMD was moderately (r=0.515) and significantly (p=0.024) correlated with V\u0307O\n                      <jats:sub>2<\/jats:sub>\n                      \u00a0peak.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_teb-2025-0006_abs_004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These novel data indicate that in healthy young adults, Notch1 activity is linked to aerobic fitness but may not be acutely involved in the shear-mediated vasodilatory response.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1515\/teb-2025-0006","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T02:58:15Z","timestamp":1748401095000},"page":"74-82","source":"Crossref","is-referenced-by-count":1,"title":["Association of circulating Notch1 and VEGF with flow-mediated dilation and aerobic fitness in healthy adults"],"prefix":"10.1515","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5111-3286","authenticated-orcid":false,"given":"Brooke R.","family":"Shepley","sequence":"first","affiliation":[{"name":"Department of Kinesiology, Faculty of Human Kinetics , 8637 University of Windsor , Windsor , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick J.","family":"Lester","sequence":"additional","affiliation":[{"name":"Department of Kinesiology, Faculty of Human Kinetics , 8637 University of Windsor , Windsor , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lana H.","family":"Yacoub","sequence":"additional","affiliation":[{"name":"Department of Kinesiology, Faculty of Human Kinetics , 8637 University of Windsor , Windsor , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony R.","family":"Bain","sequence":"additional","affiliation":[{"name":"Department of Kinesiology, Faculty of Human Kinetics , 8637 University of Windsor , Windsor , ON , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,5,28]]},"reference":[{"key":"2026062610003518866_j_teb-2025-0006_ref_001","doi-asserted-by":"crossref","unstructured":"Versari, D, Daghini, E, Virdis, A, Ghiadoni, L, Taddei, S. 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