{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T18:58:32Z","timestamp":1649098712540},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"6","license":[{"start":{"date-parts":[[1990,6,1]],"date-time":"1990-06-01T00:00:00Z","timestamp":644198400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1990,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This in vitro study focuses on the UVA-induced reactions with DNA of thioridazine (TRZ), and two of its major metabolites (TRZ-2-sulfoxide or mesoridazine, MRZ ; and TRZ- 2-sulfone or sulforidazine, SRZ). TRZ binds covalently to DNA upon UVA-irradiation. Under comparable irradiation conditions, MRZ binds to a lesser extent and almost no binding was observed with SRZ. Besides, photo-induced genetic effects were investigated by means of a differential DNA repair test in E. coli. The photo-induced genetic effects in E. coli decreased from TRZ, MRZ to SRZ, which corresponds with their capacity for UVA-induced binding to DNA. TRZ, MRZ and SRZ differed in their rate of photodecomposition rather than in the intrinsic reactivity towards DNA of the instable intermediates formed.<\/jats:p>\n               <jats:p>Irreversible binding to DNA was also observed upon treatment with peroxidase, which is known to oxidize phenothiazines via the formation of reactive radical cation species. As both the colour of the intermediate and its reactivity towards DNA were comparable for peroxidase treatment and exposure to UVA, we assume that the radical cation is the reactive species in the latter case as well.<\/jats:p>","DOI":"10.1515\/znc-1990-0613","type":"journal-article","created":{"date-parts":[[2018,8,2]],"date-time":"2018-08-02T17:54:55Z","timestamp":1533232495000},"page":"638-644","source":"Crossref","is-referenced-by-count":2,"title":["UVA-Induced Genetic Effects of Thioridazine, Mesoridazine and Sulforidazine: an in vitro Study"],"prefix":"10.1515","volume":"45","author":[{"given":"Simon A.","family":"Schoonderwoerd","sequence":"first","affiliation":[{"name":"Department of Medicinal Chemistry, Center for Bio-Pharmaceutical Sciences, Leiden University. P.O. Box 9502, 2300 RA Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerard M. J. Beijersbergen","family":"van Henegouwen","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, Center for Bio-Pharmaceutical Sciences, Leiden University. P.O. Box 9502, 2300 RA Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan R.","family":"Panday","sequence":"additional","affiliation":[{"name":"Department of Medicinal Chemistry, Center for Bio-Pharmaceutical Sciences, Leiden University. P.O. Box 9502, 2300 RA Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2014,6,2]]},"container-title":["Zeitschrift f\u00fcr Naturforschung C"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.degruyter.com\/view\/j\/znc.1990.45.issue-6\/znc-1990-0613\/znc-1990-0613.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-1990-0613\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-1990-0613\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T14:10:22Z","timestamp":1624371022000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-1990-0613\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2014,6,2]]},"published-print":{"date-parts":[[1990,6,1]]}},"alternative-id":["10.1515\/znc-1990-0613"],"URL":"https:\/\/doi.org\/10.1515\/znc-1990-0613","relation":{},"ISSN":["1865-7125","0939-5075"],"issn-type":[{"value":"1865-7125","type":"electronic"},{"value":"0939-5075","type":"print"}],"subject":[],"published":{"date-parts":[[1990,6,1]]}}}