{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:09:29Z","timestamp":1762866569529},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"11-12","license":[{"start":{"date-parts":[[2000,12,1]],"date-time":"2000-12-01T00:00:00Z","timestamp":975628800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,12,1]]},"abstract":"<jats:p>A highly sensitive fluorometric assay for the determination of monooxygenase activity in liver microsomes is described. The assay is based on the use of 3-chloro-7-methoxy-4-methylcoumarin which is demethylated to 3-chloro-7-hydroxy-4-methylcoumarin. The rate of formation of 3-chloro-7-hydroxy-4-methylcoumarin was recorded as an increase of fluorescence (\u03bb<jats:sub>A<\/jats:sub> = 380 nm, \u03bb<jats:sub>F<\/jats:sub> = 480 nm ) with time. When 3-chloro-7-methoxy-4-methylcoumarin was incubated in the presence of MgCl<jats:bold>\n                     <jats:sub>2<\/jats:sub>\n                  <\/jats:bold>and NADPH with rat liver microsomes, a continuous increase of the fluorescence could be measured. The reaction proceeded linearly for about 10 min and at least up to a concentration of 0.1 mg\/ml of microsomal protein. Besides 3-chloro-7-hydroxy-4-methylcoumarin a hydroxylated derivative of the substrate was formed as a second metabolite during the incubation. Using an excitation wavelength of 480 nm and a fluorescence\/em ission w avelength of 480 nm, the fluorescence of this substance (\u03bb<jats:sub>A<\/jats:sub> = 338 nm, \u03bb<jats:sub>F<\/jats:sub> = 422 nm ) amounted only to about 1% of the fluorescence of the main product. The use of 3-chloro-7-methoxy-4-methylcoumarin as substrate enables the fluorometric determination of the O-dealkylation activity of a cytochrom e P450-dependent monooxygenase system in rat liver which is inducible by phenobarbital but not by 3-methylcholanthrene.<\/jats:p>","DOI":"10.1515\/znc-2000-11-1212","type":"journal-article","created":{"date-parts":[[2015,2,5]],"date-time":"2015-02-05T13:47:59Z","timestamp":1423144079000},"page":"915-922","source":"Crossref","is-referenced-by-count":5,"title":["A Direct, Highly Sensitive Fluorometric Assay for a Microsomal Cytochrome P450-Mediated O-Demethylation Using a Novel Coumarin Analog as Substrate"],"prefix":"10.1515","volume":"55","author":[{"given":"Ren\u00e9","family":"Roser","sequence":"first","affiliation":[{"name":"Abteilung Physiologische Chemie der Universit\u00e4t Ulm, Albert-Einstein-Allee 11, D-89069 Ulm"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helmut","family":"Thomas","sequence":"additional","affiliation":[{"name":"Abteilung Physiologische Chemie der Universit\u00e4t Ulm, Albert-Einstein-Allee 11, D-89069 Ulm"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2014,6,2]]},"container-title":["Zeitschrift f\u00fcr Naturforschung C"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.degruyter.com\/view\/j\/znc.2000.55.issue-11-12\/znc-2000-11-1212\/znc-2000-11-1212.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2000-11-1212\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2000-11-1212\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T14:05:06Z","timestamp":1624370706000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2000-11-1212\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,12,1]]},"references-count":0,"journal-issue":{"issue":"11-12","published-online":{"date-parts":[[2014,6,2]]},"published-print":{"date-parts":[[2000,12,1]]}},"alternative-id":["10.1515\/znc-2000-11-1212"],"URL":"https:\/\/doi.org\/10.1515\/znc-2000-11-1212","relation":{},"ISSN":["1865-7125","0939-5075"],"issn-type":[{"value":"1865-7125","type":"electronic"},{"value":"0939-5075","type":"print"}],"subject":[],"published":{"date-parts":[[2000,12,1]]}}}