{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:45:42Z","timestamp":1790358342410,"version":"4.1.0"},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"9-10","license":[{"start":{"date-parts":[[2004,10,1]],"date-time":"2004-10-01T00:00:00Z","timestamp":1096588800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p> Salicylic acid \u03b2-glucoside (SAG) is a storage form of a defense signal against pathogens, releasing free salicylic acid (SA), to meet the requirements in plants. Since excess SA induces locally restricted cell death following oxidative burst and Ca<jats:sup>2+<\/jats:sup> influx in plants, the effects of SAG on cell viability, Ca<jats:sup>2+<\/jats:sup> influx, and generation of superoxide (O<jats:sup>\u2022\u2212<\/jats:sup>) were examined in suspension-cultured tobacco BY-2 cells expressing aequorin. Among SA-related chemicals tested, only SAG induced the slow and long-lasting O<jats:sub>2<\/jats:sub>\n                  <jats:sup>\u2022\u2212<\/jats:sup> generation, although SAG was less active in acute O<jats:sub>2<\/jats:sub>\n                  <jats:sup>\u2022\u2212<\/jats:sup> generation, Ca2+ influx and induction of cell death. The prolonging action of SAG is likely due to gradual release of SA and the data suggested that a peroxidasedependent reaction is involved. Notably, pretreatment with low-dose SA (50 \u03bcm) enhanced the response to SAG by 2.5-fold. There are four possible secondary messengers in early SA signaling detectable in the BY-2 culture, namely O<jats:sub>2<\/jats:sub>\n                  <jats:sup>\u2022\u2212<\/jats:sup>, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, Ca<jats:sup>2+<\/jats:sup> and protein kinase (PK). If these messengers are involved in the low-dose SA-dependent priming for SAG response, they should be inducible by low-dose SA. Among the four SA-inducible signaling events, PK activation was excluded from the low-dose SA action since a much higher SA dose (&gt; 0.4 m\u043c) was required for PK activation. <\/jats:p>","DOI":"10.1515\/znc-2004-9-1013","type":"journal-article","created":{"date-parts":[[2015,2,4]],"date-time":"2015-02-04T12:59:31Z","timestamp":1423054771000},"page":"684-692","source":"Crossref","is-referenced-by-count":29,"title":["Salicylic Acid Glucoside Acts as a Slow Inducer of Oxidative Burst in Tobacco Suspension Culture"],"prefix":"10.1515","volume":"59","author":[{"given":"Tomonori","family":"Kawano","sequence":"first","affiliation":[{"name":"Graduate School of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0157, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeo","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Bioscience, Faculty of Applied Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Kadono","sequence":"additional","affiliation":[{"name":"Graduate School of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0157, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoshi","family":"Muto","sequence":"additional","affiliation":[{"name":"Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2014,6,2]]},"container-title":["Zeitschrift f\u00fcr Naturforschung C"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyter.com\/view\/journals\/znc\/59\/9-10\/article-p684.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2004-9-1013\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2004-9-1013\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,23]],"date-time":"2022-08-23T05:13:25Z","timestamp":1661231605000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2004-9-1013\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,10,1]]},"references-count":0,"journal-issue":{"issue":"9-10","published-online":{"date-parts":[[2014,6,2]]},"published-print":{"date-parts":[[2004,10,1]]}},"alternative-id":["10.1515\/znc-2004-9-1013"],"URL":"https:\/\/doi.org\/10.1515\/znc-2004-9-1013","relation":{},"ISSN":["1865-7125","0939-5075"],"issn-type":[{"value":"1865-7125","type":"electronic"},{"value":"0939-5075","type":"print"}],"subject":[],"published":{"date-parts":[[2004,10,1]]}}}