{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T11:27:08Z","timestamp":1761391628837},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"3-4","license":[{"start":{"date-parts":[[2005,4,1]],"date-time":"2005-04-01T00:00:00Z","timestamp":1112313600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p> A functional screening in yeast allowed to identify various cDNAs from the Cd\/Zn hyperaccumulator Thlaspi caerulescens. TcMT3 displayed high identity with its closest homologue in Arabidopsis thaliana but variation in its Cys residues. Functional analysis in yeast supported a higher binding capacity for Cu, but not for Cd or Zn, of TcMT3 compared to AtMT3. Expression analysis in plants indicated that metallothionein 3 (MT3) like all the other T. caerulescens genes from the screen studied is overexpressed in all studied populations of T. caerulescens compared to A. thaliana. TcMT3 was induced by Cu, but not by Cd. Moreover significant variation in expression within T. caerulescens populations that have contrasting tolerance and accumulation capacities indicated a possible local adaptation of MT3.<\/jats:p>","DOI":"10.1515\/znc-2005-3-407","type":"journal-article","created":{"date-parts":[[2015,2,4]],"date-time":"2015-02-04T13:00:35Z","timestamp":1423054835000},"page":"224-228","source":"Crossref","is-referenced-by-count":6,"title":["Adaptative Evolution of Metallothionein 3 in the Cd\/Zn Hyperaccumulator Thlaspi caerulescens"],"prefix":"10.1515","volume":"60","author":[{"given":"Nancy H.","family":"Roosens","sequence":"first","affiliation":[{"name":"Laboratoire de Physiologie et G\u00e9n\u00e9tique Mol\u00e9culaire des Plantes, Universit\u00e9 Libre de Bruxelles, Campus Plaine (CP 242) Bd du triomphe, B-1050 Brussels, Belgium. Fax: (32)(0)26505421."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Bernard","sequence":"additional","affiliation":[{"name":"Laboratoire de Physiologie et G\u00e9n\u00e9tique Mol\u00e9culaire des Plantes, Universit\u00e9 Libre de Bruxelles, Campus Plaine (CP 242) Bd du triomphe, B-1050 Brussels, Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raphael","family":"Leplae","sequence":"additional","affiliation":[{"name":"Service de Conformation des Macromol\u00e9cules Biologiques et Bioinformatique, Universit\u00e9 Libre de Bruxelles, Campus Plaine (CP 263) Bd du triomphe, B-1050 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Verbruggen","sequence":"additional","affiliation":[{"name":"Laboratoire de Physiologie et G\u00e9n\u00e9tique Mol\u00e9culaire des Plantes, Universit\u00e9 Libre de Bruxelles, Campus Plaine (CP 242) Bd du triomphe, B-1050 Brussels, Belgium."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2014,6,2]]},"container-title":["Zeitschrift f\u00fcr Naturforschung C"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyter.com\/view\/journals\/znc\/60\/3-4\/article-p224.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2005-3-407\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2005-3-407\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,23]],"date-time":"2022-08-23T05:09:30Z","timestamp":1661231370000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2005-3-407\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4,1]]},"references-count":0,"journal-issue":{"issue":"3-4","published-online":{"date-parts":[[2014,6,2]]},"published-print":{"date-parts":[[2005,4,1]]}},"alternative-id":["10.1515\/znc-2005-3-407"],"URL":"https:\/\/doi.org\/10.1515\/znc-2005-3-407","relation":{},"ISSN":["1865-7125","0939-5075"],"issn-type":[{"value":"1865-7125","type":"electronic"},{"value":"0939-5075","type":"print"}],"subject":[],"published":{"date-parts":[[2005,4,1]]}}}