{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T14:35:22Z","timestamp":1791124522086,"version":"4.1.0"},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"1-2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,2,1]]},"abstract":"<jats:p>\n                    Lichen phenolic compounds exhibit antioxidant, antimicrobial, antiproliferative, and cytotoxic activities. The purpose of this study was to evaluate the anticancer activity of lecanoric acid, a secondary metabolite of the lichen Parmotrema tinctorum, and its derivatives, orsellinates, obtained by structural modifi cation. A cytotoxicity assay was carried out in vitro with sulforhodamine B (SRB) using HEp-2 larynx carcinoma, MCF7 breast carcinoma, 786-0 kidney carcinoma, and B16-F10 murine melanoma cell lines, in addition to a normal (Vero) cell line in order to calculate the selectivity index of the compounds. n-Butyl orsellinate was the most active compound, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values (the concentration that inhibits 50% of growth) ranging from 7.2 to 14.0 \u03bcg\/mL, against all the cell lines tested. The compound was more active (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 11.4 \u03bcg\/mL) against B16-F10 cells than was cisplatin (12.5 \u03bcg\/mL). Conversely, lecanoric acid and methyl orsellinate were less active against all cell lines, having an IC\n                    <jats:sub>50<\/jats:sub>\n                    value higher than 50 \u03bcg\/mL. Ethyl orsellinate was more active against HEp-2 than against MCF7, 786-0, or B16-F10 cells. The same pattern was observed for n-propyl and n-butyl orsellinates. n-Pentyl orsellinate was less active than n-propyl or n-butyl orsellinates against HEp-2 cells. The orsellinate activity increased with chain elongation (from methyl to n-butyl), a likely consequence of an increase in lipophilicity. The results revealed that the structural modifi cation of lecanoric acid increases the cytotoxic activity of the derivatives tested.\n                  <\/jats:p>","DOI":"10.1515\/znc-2010-1-208","type":"journal-article","created":{"date-parts":[[2015,1,30]],"date-time":"2015-01-30T07:36:36Z","timestamp":1422603396000},"page":"43-48","source":"Crossref","is-referenced-by-count":37,"title":["In vitro Antitumour Activity of Orsellinates"],"prefix":"10.1515","volume":"65","author":[{"given":"Danielle","family":"Bogo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular e Culturas Celulares, Departamento de Farm\u00e1cia \u2013 Bioqu\u00edmica, Universidade Federal de Mato Grosso do Sul (UFMS), Caixa Postal 549, Campo Grande, MS 79070-900, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria de Fatima Cepa","family":"Matos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular e Culturas Celulares, Departamento de Farm\u00e1cia \u2013 Bioqu\u00edmica, Universidade Federal de Mato Grosso do Sul (UFMS), Caixa Postal 549, Campo Grande, MS 79070-900, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neli Kika","family":"Honda","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, UFMS, Campo Grande, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elenir Curi","family":"Pontes","sequence":"additional","affiliation":[{"name":"Departamento de Tecnologia de Alimentos e Sa\u00fade P\u00fablica, UFMS, Campo Grande, MS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia Midori","family":"Oguma","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular e Culturas Celulares, Departamento de Farm\u00e1cia \u2013 Bioqu\u00edmica, Universidade Federal de Mato Grosso do Sul (UFMS), Caixa Postal 549, Campo Grande, MS 79070-900, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn Cristina da Silva","family":"Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular e Culturas Celulares, Departamento de Farm\u00e1cia \u2013 Bioqu\u00edmica, Universidade Federal de Mato Grosso do Sul (UFMS), Caixa Postal 549, Campo Grande, MS 79070-900, Brazil."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Ernesto de","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Centro Pluridisciplinar de Pesquisas Qu\u00edmicas, Biol\u00f3gicas e Agr\u00edcolas, Universidade Estadual de Campinas (Unicamp), Caixa Postal 6171, Campinas, SP 13081-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Auro","family":"Nomizo","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Universidade de S\u00e3o Paulo (USP), Avenida Professor Zeferino Vaz, s\/n., Ribeir\u00e3o Preto, SP 14040-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2014,6,2]]},"container-title":["Zeitschrift f\u00fcr Naturforschung C"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.degruyter.com\/view\/j\/znc.2010.65.issue-1-2\/znc-2010-1-208\/znc-2010-1-208.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/znc-2010-1-208\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/znc-2010-1-208\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T09:12:13Z","timestamp":1765789933000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/znc-2010-1-208\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2,1]]},"references-count":0,"journal-issue":{"issue":"1-2","published-online":{"date-parts":[[2014,6,2]]},"published-print":{"date-parts":[[2010,2,1]]}},"alternative-id":["10.1515\/znc-2010-1-208"],"URL":"https:\/\/doi.org\/10.1515\/znc-2010-1-208","relation":{},"ISSN":["1865-7125","0939-5075"],"issn-type":[{"value":"1865-7125","type":"electronic"},{"value":"0939-5075","type":"print"}],"subject":[],"published":{"date-parts":[[2010,2,1]]}}}