{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T22:12:10Z","timestamp":1779919930050,"version":"3.53.1"},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"7-8","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,8,1]]},"abstract":"<jats:p>Application of acetylcholinesterase (AChE) inhibitors is the primary treatment for Alzheimer\u2019s disease. Alkaloids, such as physostigmine, galanthamine, and huperzine A, play an important role as AChE inhibitors. The aim of this work was to evaluate Himatanthus lancifolius (Muell. Arg.) Woodson, a Brazilian species of Apocynaceae, and its main indole alkaloid uleine, in order to identify new AChE inhibitors. The plant fluid extract, fractions, and uleine were tested for AChE inhibitory activity using Ellman\u2019s colorimetric method for thin-layer chromatography (TLC), 96-well microplates, and also Marston\u2019s TLC colorimetric method. Both TLC assays showed similar results. At 5 mg\/mL, the fluid extract inhibited the AChE enzyme by (50.71 \u00b1 8.2)%. The ethyl acetate fraction exhibited the highest level of AChE inhibition, followed by the dichloromethane fraction. The isolated alkaloid uleine displayed an IC<jats:sub>50<\/jats:sub> value of 0.45 \u03bcM.<\/jats:p>","DOI":"10.1515\/znc-2010-7-804","type":"journal-article","created":{"date-parts":[[2015,1,30]],"date-time":"2015-01-30T12:36:36Z","timestamp":1422621396000},"page":"440-444","source":"Crossref","is-referenced-by-count":35,"title":["Acetylcholinesterase Inhibitory Activity of Uleine from Himatanthus lancifolius"],"prefix":"10.1515","volume":"65","author":[{"given":"Cl\u00e1udia","family":"Seidl","sequence":"first","affiliation":[{"name":"Laboratory of Pharmacognosy, Pharmacy Department, Universidade Federal do Paran\u00e1, Rua Prof. Loth\u00e1rio Meissner, 632 \u2013 Jd. Bot\u00e2nico 80.210-170, Curitiba \u2013 PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz L.","family":"Correia","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacognosy, Pharmacy Department, Universidade Federal do Paran\u00e1, Rua Prof. Loth\u00e1rio Meissner, 632 \u2013 Jd. Bot\u00e2nico 80.210-170, Curitiba \u2013 PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9a E. M.","family":"Stinghen","sequence":"additional","affiliation":[{"name":"LabMicro, Departamento de Patologia B\u00e1sica, Setor de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Paran\u00e1, Caixa Postal 19.031, Centro Polit\u00e9cnico 80.531-980, Curitiba \u2013 PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cid A. M.","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacognosy, Pharmacy Department, Universidade Federal do Paran\u00e1, Rua Prof. Loth\u00e1rio Meissner, 632 \u2013 Jd. Bot\u00e2nico 80.210-170, Curitiba \u2013 PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2014,6,2]]},"container-title":["Zeitschrift f\u00fcr Naturforschung C"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.degruyter.com\/view\/j\/znc.2010.65.issue-7-8\/znc-2010-7-804\/znc-2010-7-804.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2010-7-804\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2010-7-804\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T14:15:20Z","timestamp":1624371320000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.1515\/znc-2010-7-804\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8,1]]},"references-count":0,"journal-issue":{"issue":"7-8","published-online":{"date-parts":[[2014,6,2]]},"published-print":{"date-parts":[[2010,8,1]]}},"alternative-id":["10.1515\/znc-2010-7-804"],"URL":"https:\/\/doi.org\/10.1515\/znc-2010-7-804","relation":{},"ISSN":["1865-7125","0939-5075"],"issn-type":[{"value":"1865-7125","type":"electronic"},{"value":"0939-5075","type":"print"}],"subject":[],"published":{"date-parts":[[2010,8,1]]}}}