{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T10:24:54Z","timestamp":1768818294951,"version":"3.49.0"},"reference-count":26,"publisher":"Walter de Gruyter GmbH","issue":"11-12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,10,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Ginkgetin is known to be an anticancer agent in many studies. However, its effectiveness in treating chronic lymphoblastic leukemia (CLL) remains unknown. The present study aimed to evaluate the effects of ginkgetin on the growth of the K562 cell line. The MTT assay was employed to examine the proliferation of K562, and a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) staining was conducted to detect the apoptotic rates. Furthermore, changes of tumor necrosis factor-\u03b1 (TNF-\u03b1) were detected by Western blot analysis. Ginkgetin inhibited the proliferation of K562 cells in a dose- and time-dependent manner. Concentrations of ginkgetin required to induce 50% death of K562 at 24, 48 and 72 h were 38.9, 31.3 and 19.2 \u03bcM, respectively. Moreover, treatment of ginkgetin increased K562 apoptosis in vitro along with increased levels of TNF-\u03b1. Interestingly, anti-TNF-\u03b1 antibody prevented ginkgetin-induced K562 cell apoptosis and growth inhibition via deactivation of caspase-8, caspase-9 and caspase-3. Concomitantly, downregulation of TNF-\u03b1 by etanercept in vivo attenuated ginkgetin-induced inhibitory effects on the tumor growth in an xenograft mouse model. Our results indicate that ginkgetin effectively inhibits K562 cell proliferation, and TNF-\u03b1 plays a key role in ginkgetin-induced cell apoptosis.<\/jats:p>","DOI":"10.1515\/znc-2016-0210","type":"journal-article","created":{"date-parts":[[2017,9,13]],"date-time":"2017-09-13T10:03:21Z","timestamp":1505297001000},"page":"441-447","source":"Crossref","is-referenced-by-count":12,"title":["Ginkgetin inhibits proliferation of human leukemia cells via the TNF-\u03b1 signaling pathway"],"prefix":"10.1515","volume":"72","author":[{"given":"Ling-Ling","family":"Pan","sequence":"first","affiliation":[{"name":"The Bone Marrow Transplantation Center and Multiple Myeloma Treatment Center , The First Affiliated Hospital of Medical College, Zhejiang University , Hangzhou 310003 , China"},{"name":"Blood Center of Zhejiang Province , Hangzhou 310006 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Jun","family":"Wu","sequence":"additional","affiliation":[{"name":"The Bone Marrow Transplantation Center and Multiple Myeloma Treatment Center , The First Affiliated Hospital of Medical College, Zhejiang University , Hangzhou 310003 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gao-Feng","family":"Zheng","sequence":"additional","affiliation":[{"name":"The Bone Marrow Transplantation Center and Multiple Myeloma Treatment Center , The First Affiliated Hospital of Medical College, Zhejiang University , Hangzhou 310003 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Yan","family":"Han","sequence":"additional","affiliation":[{"name":"The Bone Marrow Transplantation Center and Multiple Myeloma Treatment Center , The First Affiliated Hospital of Medical College, Zhejiang University , Hangzhou 310003 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing-Song","family":"He","sequence":"additional","affiliation":[{"name":"The Bone Marrow Transplantation Center and Multiple Myeloma Treatment Center , The First Affiliated Hospital of Medical College, Zhejiang University , Hangzhou 310003 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Cai","sequence":"additional","affiliation":[{"name":"The Bone Marrow Transplantation Center and Multiple Myeloma Treatment Center , The First Affiliated Hospital of Medical College, Zhejiang University , Hangzhou 310003 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2017,9,13]]},"reference":[{"key":"2021062203195922495_j_znc-2016-0210_ref_001_w2aab3b7c41b1b6b1ab2b2b1Aa","doi-asserted-by":"crossref","unstructured":"Rai KR, Jain P. 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