{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T02:53:07Z","timestamp":1788576787206,"version":"build-2803163510"},"reference-count":28,"publisher":"Walter de Gruyter GmbH","issue":"11-12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    This study focuses on the microbial biotransformation of vinblastine to vincristine, a compound with enhanced anticancer efficacy compared to vinblastine utilizing a fungal endophyte\n                    <jats:italic>Fusarium equiseti<\/jats:italic>\n                    , isolated from\n                    <jats:italic>Catharanthus roseus<\/jats:italic>\n                    . Additionally, the antiangiogenic properties of the resulting vincristine were evaluated\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    using the chick chorioallantoic membrane assay. The biotransformation was conducted in liquid medium containing vinblastine (0.6\u20132.4\u202fmM), and\n                    <jats:italic>F.\u00a0equiseti<\/jats:italic>\n                    spore suspension (50\u2013150\u202f\u00b5L) by incubating at 30\u202f\u00b0C, pH 5.6, and 200\u202frpm for eight days. After the reaction was over, products were extracted using methanol and analyzed by LC-MS\/MS. The results confirmed the production of vincristine from vinblastine in different sets of biotransformation reactions. In biotransformation reaction-1, which comprised of vinblastine (0.6\u202fmM) and fungal inoculum 150\u202f\u00b5L (82\u00a0\u00d7\u00a010\n                    <jats:sup>4<\/jats:sup>\n                    \u202fspores\/mL), 92\u202f% of vinblastine was converted into vincristine on 8th day of incubation. The vincristine produced demonstrated significant antiangiogenetic properties in the chick chorioallantoic membrane assay in a dose-dependent manner. The antiangiogenic properties of vincristine produced was comparable to standard vincristine. Hence, the study revealed that\n                    <jats:italic>F.\u00a0equiseti<\/jats:italic>\n                    present a viable alternative to produce vincristine from vinblastine. To our knowledge, this is the first report on\n                    <jats:italic>F.\u00a0equiseti<\/jats:italic>\n                    highlighting its vinblastine biotransformation potential.\n                  <\/jats:p>","DOI":"10.1515\/znc-2024-0263","type":"journal-article","created":{"date-parts":[[2025,3,22]],"date-time":"2025-03-22T03:52:12Z","timestamp":1742615532000},"page":"751-762","source":"Crossref","is-referenced-by-count":1,"title":["Biotransformation of vinblastine into vincristine using a fungal endophyte\n                    <i>Fusarium equiseti<\/i>\n                    along with the assessment of its antiangiogenic properties"],"prefix":"10.1515","volume":"80","author":[{"given":"Gauri","family":"Srivastava","sequence":"first","affiliation":[{"name":"Amity Institute of Biotechnology , 531065 Amity University Uttar Pradesh , Sector 125 , Noida , 201303 , Uttar Pradesh , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruchika","family":"Mittal","sequence":"additional","affiliation":[{"name":"Amity Institute of Biotechnology , 531065 Amity University Uttar Pradesh , Sector 125 , Noida , 201303 , Uttar Pradesh , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhi","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Amity Institute of Biotechnology , 531065 Amity University Uttar Pradesh , Sector 125 , Noida , 201303 , Uttar Pradesh , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarita","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Botany, C.M.P. Degree College , University of Allahabad, Mahatma Gandhi Marg , George Town , Prayagraj , 211002 , Uttar Pradesh , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3911-1333","authenticated-orcid":false,"given":"Deepak","family":"Ganjewala","sequence":"additional","affiliation":[{"name":"Amity Institute of Biotechnology , 531065 Amity University Uttar Pradesh , Sector 125 , Noida , 201303 , Uttar Pradesh , India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,3,24]]},"reference":[{"key":"2025110310023727578_j_znc-2024-0263_ref_001","unstructured":"Gajalakshmi, S, Vijayalakshmi, S, Devi, RV. Pharmacological activities of Catharanthus roseus: a perspective review. Int J Pharm Bio Sci 2013;4:431\u20139."},{"key":"2025110310023727578_j_znc-2024-0263_ref_002","doi-asserted-by":"crossref","unstructured":"Nelson, RL. 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