{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T14:17:22Z","timestamp":1778595442095,"version":"3.51.4"},"reference-count":26,"publisher":"Walter de Gruyter GmbH","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A new pyranocoumarin, Calofrasolide A (\n                    <jats:bold>1<\/jats:bold>\n                    ), was isolated from the stem bark of\n                    <jats:italic>Calophyllum fraseri<\/jats:italic>\n                    , together with the known constituents \u03b2-sitosterol, stigmasterol, friedelin, and lupeol. The structure of the new compound was established through comprehensive spectroscopic analyses and confirmed by comparison with reported data. Compound\n                    <jats:bold>1<\/jats:bold>\n                    was evaluated for antimicrobial and antibiofilm activities using microdilution and crystal-violet assays. It exhibited notable antimicrobial activity, with MIC values of 62.5\u202f\u03bcg\/mL against\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    and\n                    <jats:italic>Streptococcus mutans<\/jats:italic>\n                    . In the antibiofilm assays, compound\n                    <jats:bold>1<\/jats:bold>\n                    demonstrated pronounced inhibitory effects, suppressing\n                    <jats:italic>C.\u00a0albicans<\/jats:italic>\n                    biofilm formation by 30.25\u00a0\u00b1\u00a01.04\u202f% and\n                    <jats:italic>S.\u00a0mutans<\/jats:italic>\n                    biofilms by 78.37\u00a0\u00b1\u00a00.22\u202f%. Molecular docking studies supported the experimental findings, revealing favourable binding (LBE\u00a0=\u00a0\u22128.5\u202fkcal\/mol) within the active sites of ERG11 (5TZ1) and GtfB (8FK4). These findings highlight Calofrasolide A as a promising pyranocoumarin scaffold with potential antimicrobial and antibiofilm relevance, particularly against oral pathogenic infections.\n                  <\/jats:p>","DOI":"10.1515\/znc-2025-0314","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:56:17Z","timestamp":1778590577000},"source":"Crossref","is-referenced-by-count":0,"title":["Calofrasolide A, a new pyranocoumarin from the stem bark of\n                    <i>Calophyllum fraseri<\/i>\n                    M.R.Hend. &amp; Wyatt-Sm. and their antimicrobial and antibiofilm activities"],"prefix":"10.1515","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0847-489X","authenticated-orcid":false,"given":"Nur Nabilah Mohd","family":"Zaini","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Mathematics , Universiti Pendidikan Sultan Idris , 35900 , Tanjong Malim , Perak , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1408-229X","authenticated-orcid":false,"given":"Wan Mohd Nuzul Hakimi Wan","family":"Salleh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Mathematics , Universiti Pendidikan Sultan Idris , 35900 , Tanjong Malim , Perak , Malaysia"},{"name":"Fraser\u2019s Hill Research Center , Universiti Kebangsaan Malaysia , 43600 , Bangi , Selangor , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3602-6216","authenticated-orcid":false,"given":"Nurunajah Ab","family":"Ghani","sequence":"additional","affiliation":[{"name":"Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns) , Universiti Teknologi MARA , Puncak Alam Campus, 42300 , Puncak Alam , Selangor , Malaysia"},{"name":"Faculty of Applied Sciences , Universiti Teknologi MARA , Shah Alam , Selangor , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4425-7524","authenticated-orcid":false,"given":"Abubakar Siddiq","family":"Salihu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science and Mathematics , Universiti Pendidikan Sultan Idris , 35900 , Tanjong Malim , Perak , Malaysia"},{"name":"Department of Pure and Industrial Chemistry, Faculty of Natural and Applied Science , Umaru Musa Yar\u2019adua University , Katsina , Nigeria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9470-6412","authenticated-orcid":false,"given":"Mohd Hafiz","family":"Arzmi","sequence":"additional","affiliation":[{"name":"Department of Fundamental Dental and Medical Sciences, Kulliyyah of Dentistry , International Islamic University Malaysia , IIUM Kuantan Campus , Pahang , Malaysia"},{"name":"Melbourne Dental School , The University of Melbourne , Parkville , 3053 , VIC , Australia"},{"name":"Oxford RMC Science Ltd. , University of Oxford , Walton Street , Oxford , OX1 2HB , UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4696-5839","authenticated-orcid":false,"given":"Nurulfazlina Edayah","family":"Rasol","sequence":"additional","affiliation":[{"name":"Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns) , Universiti Teknologi MARA , Puncak Alam Campus, 42300 , Puncak Alam , Selangor , Malaysia"},{"name":"Faculty of Applied Sciences , Universiti Teknologi MARA , Shah Alam , Selangor , Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6667-9245","authenticated-orcid":false,"given":"Sherali","family":"Kuziev","sequence":"additional","affiliation":[{"name":"Department of Biochemistry , National University of Uzbekistan , 100174 , Tashkent , Uzbekistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8534-6235","authenticated-orcid":false,"given":"Feruzbek","family":"Khasanov","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Chemistry , Alfraganus University , 100190 , Tashkent , Uzbekistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","published-online":{"date-parts":[[2026,5,12]]},"reference":[{"key":"2026051213242385750_j_znc-2025-0314_ref_001","doi-asserted-by":"crossref","unstructured":"Gupta, S, Gupta, P. 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