{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T13:26:36Z","timestamp":1765545996212,"version":"3.48.0"},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    We recently reported protection against metabolic syndrome (MetSyn) induction and endothelial dysfunction by natural mineral-rich water intake in fructose-fed Sprague-Dawley rats. As glucocorticoids are critical to MetSyn development, we aimed to further characterize the beneficial effects of mineral-rich water intake in that animal model, by assessing relevant effectors in glucocorticoid-signaling in liver and subcutaneous (SCAT) and visceral (VAT) adipose tissues, sites with a central role in metabolic (dys)regulation.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Materials and methods:<\/jats:bold>\n                    Adult male rats had free access to standard diet and different drinking solutions (8 weeks): a) tap water (CONT), b) 10% fructose\/tap water (FRUCT) or c) 10% fructose\/mineral-rich water (FRUCTMIN). 11\u03b2-hydroxysteroid dehydrogenase type 1 (11\u03b2-HSD1), glucocorticoid receptor (GR), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1-\u03b1) and sirtuin 1 (Sirt1) tissue protein expressions were evaluated by Western blot. Plasma corticosterone (ELISA) and non-esterified fatty acids (NEFA) levels were quantified spectrophotometrically.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Expectedly, Sirt1 and PGC1-\u03b1 significantly decreased in liver, 11\u03b2-HSD1 tended to increase in VAT and tended to decrease in liver and SCAT, and plasma corticosterone tended to increase in FRUCT vs. CONT. Mineral-rich water showed a trend towards a reduction of these fructose effects and significantly increased hepatic Sirt1 vs. CONT and FRUCT. GR significantly increased in VAT and plasma NEFA strongly tended to increase in FRUCTMIN vs. CONT and FRUCT.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    Glucocorticoid-signaling was different among SCAT and VAT and also in liver. Mineral-rich water modulation of fructose effects on glucocorticoid-signaling and Sirt1 underlines the better metabolic profile found earlier.\n                  <\/jats:p>","DOI":"10.1515\/hmbci-2014-0032","type":"journal-article","created":{"date-parts":[[2015,3,6]],"date-time":"2015-03-06T04:33:26Z","timestamp":1425616406000},"page":"149-157","source":"Crossref","is-referenced-by-count":12,"title":["Natural mineral-rich water ingestion improves hepatic and fat glucocorticoid-signaling and increases sirtuin 1 in an animal model of metabolic syndrome"],"prefix":"10.1515","volume":"21","author":[{"given":"Cid\u00e1lia D.","family":"Pereira","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Biochemistry (U38\/FCT), University of Porto, Porto, Portugal"}]},{"given":"Milton","family":"Severo","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Clinical Epidemiology, Predictive Medicine and Public Health and University of Porto, Porto, Portugal"}]},{"given":"Delminda","family":"Neves","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio","family":"Ascens\u00e3o","sequence":"additional","affiliation":[{"name":"CIAFEL\u2013Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal"}]},{"given":"Jos\u00e9","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"CIAFEL\u2013Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal"}]},{"given":"Jo\u00e3o T.","family":"Guimar\u00e3es","sequence":"additional","affiliation":[]},{"given":"Ros\u00e1rio","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Biochemistry (U38\/FCT), University of Porto, Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3560-3261","authenticated-orcid":false,"given":"Maria Jo\u00e3o","family":"Martins","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Biochemistry (U38\/FCT), University of Porto, Porto, Portugal"}]}],"member":"374","published-online":{"date-parts":[[2015,1,13]]},"container-title":["Hormone Molecular Biology and Clinical Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/hmbci-2014-0032\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/hmbci-2014-0032\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T08:10:22Z","timestamp":1765527022000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyterbrill.com\/document\/doi\/10.1515\/hmbci-2014-0032\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,13]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,2,18]]},"published-print":{"date-parts":[[2015,2,1]]}},"alternative-id":["10.1515\/hmbci-2014-0032"],"URL":"https:\/\/doi.org\/10.1515\/hmbci-2014-0032","relation":{},"ISSN":["1868-1891","1868-1883"],"issn-type":[{"type":"electronic","value":"1868-1891"},{"type":"print","value":"1868-1883"}],"subject":[],"published":{"date-parts":[[2015,1,13]]}}}