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The effects of hypoxia and serum deprivation on the expression of glycosyltransferases and glycan profile were evaluated in the MDA-MB-231 cell line. We showed that the overexpression of HIF-1\u03b1 was accompanied by acquisition of epithelial-mesenchimal transition features. Significant upregulation of fucosyl- and sialyltransferases involved in the synthesis of tumor-associated carbohydrate antigens was observed together with changes in fucosylation and sialylation detected by <jats:italic>Aleuria aurantia<\/jats:italic> lectin and <jats:italic>Sambucus nigra<\/jats:italic> agglutinin lectin blots. Bioinformatic analysis further indicated a mechanism by which HIF-1\u03b1 can regulate <jats:italic>ST3GAL6<\/jats:italic> expression and the relationship within the intrinsic characteristics of TNBC tumors. In conclusion, our results showed the involvement of hypoxia and serum deprivation in glycosylation profile regulation of TNBC cells triggering breast cancer aggressive features and suggesting glycosylation as a potential diagnostic and therapeutic target.<\/jats:p>","DOI":"10.1515\/hsz-2018-0121","type":"journal-article","created":{"date-parts":[[2018,6,15]],"date-time":"2018-06-15T07:41:53Z","timestamp":1529048513000},"page":"661-672","source":"Crossref","is-referenced-by-count":14,"title":["Hypoxia and serum deprivation induces glycan alterations in triple negative breast cancer cells"],"prefix":"10.1515","volume":"399","author":[{"given":"Amanda P.B.","family":"Albuquerque","sequence":"first","affiliation":[{"name":"Biomarkers in Cancer Research Group (BmC) \u2013 Federal University of Pernambuco (UFPE) , 50670-901 Recife, Pernambuco , Brazil"},{"name":"Department of Biochemistry , Federal University of Pernambuco (UFPE) , 50670-901 Recife, Pernambuco , Brazil"}]},{"given":"Meritxell","family":"Balma\u00f1a","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade , Universidade do Porto , 4200-135 Porto , Portugal"},{"name":"Institute of Molecular Pathology and Immunology , University of Porto , 4200-135 Porto , Portugal"}]},{"given":"Stefan","family":"Mereiter","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade , Universidade do Porto , 4200-135 Porto , Portugal"},{"name":"Institute of Molecular Pathology and Immunology , University of Porto , 4200-135 Porto , Portugal"}]},{"given":"Filipe","family":"Pinto","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade , Universidade do Porto , 4200-135 Porto , Portugal"},{"name":"Institute of Molecular Pathology and Immunology , University of Porto , 4200-135 Porto , Portugal"}]},{"given":"Celso A.","family":"Reis","sequence":"additional","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade , Universidade do Porto , 4200-135 Porto , Portugal"},{"name":"Institute of Molecular Pathology and Immunology , University of Porto , 4200-135 Porto , Portugal"},{"name":"Institute of Biomedical Sciences Abel Salazar , University of Porto , 4050-313 Porto , Portugal"},{"name":"Faculty of Medicine , University of Porto , 4200-319 Porto , Portugal"}]},{"given":"Eduardo I.C.","family":"Beltr\u00e3o","sequence":"additional","affiliation":[{"name":"Biomarkers in Cancer Research Group (BmC) \u2013 Federal University of Pernambuco (UFPE) , 50670-901 Recife, Pernambuco , Brazil"},{"name":"Department of Biochemistry , Federal University of Pernambuco (UFPE) , 50670-901 Recife, Pernambuco , Brazil"}]}],"member":"374","published-online":{"date-parts":[[2018,6,12]]},"reference":[{"key":"2023040406490843748_j_hsz-2018-0121_ref_001_w2aab3b7c60b1b6b1ab2b1b1Aa","doi-asserted-by":"crossref","unstructured":"Alley, W.R. 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