{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T05:46:46Z","timestamp":1663825606841},"reference-count":21,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The 12 principles of green chemistry were stated to pursue good practices and techniques that minimize the use of hazardous substances in the production and application of chemicals. To minimize the risks and the pollution generated using chemicals, new methodologies must be developed that avoid the application of dangerous products or its amount. Considering the principle of pollution prevention, this work proposes the development and application of a different methodology for the quantification of humic substances (HS), applying a spectrophotometric methodology that reduces the quantity of hazardous reagents (sodium dichromate and sulfuric acid) and minimizes the waste production. To deeply acknowledge the method, a validation of the methodology was done to allow the evaluation of its effectiveness to report reliable results. It was done by the evaluation of the parameters often used for this purpose (analytical limits, precision, accuracy, selectivity, robustness, and method working range). The detection limit attained was 0.03\u2009g\u2009glucose\/L and the quantification limit was 0.09\u2009g\u2009glucose\/L. The precision and accuracy were also evaluated using the repeatability limit and through performance testing, respectively, with a repeatability limit of 0.045\u2009g\/L and a <jats:italic>Z<\/jats:italic>-score less than 2. For selectivity and robustness, differences in variances were not significant, and the working range is well adjusted, with no differences regarding the precision of the lower and higher standard. The results obtained showed that the method under study, adapted to the green chemistry principles, can be applied to quantify HS in impure and complex samples.<\/jats:p>","DOI":"10.1515\/opag-2022-0120","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T16:30:32Z","timestamp":1663086632000},"page":"679-687","source":"Crossref","is-referenced-by-count":0,"title":["Validation of an analytical methodology to determine humic substances using low-volume toxic reagents"],"prefix":"10.1515","volume":"7","author":[{"given":"Isabel","family":"Br\u00e1s","sequence":"first","affiliation":[{"name":"Environmental Department, ESTGV, Polytechnique Institute of Viseu, Campus Polit\u00e9cnico de Repeses , Viseu , 3504-510 , Portugal"},{"name":"Centre for Research in Digital Services (CISeD), Polytechnique Institute of Viseu , Viseu , 3504-510 , Portugal"}]},{"given":"Ana","family":"Tavares","sequence":"additional","affiliation":[{"name":"Environmental Department, ESTGV, Polytechnique Institute of Viseu, Campus Polit\u00e9cnico de Repeses , Viseu , 3504-510 , Portugal"}]},{"given":"Jos\u00e9","family":"Pereira","sequence":"additional","affiliation":[{"name":"Animal Science, Rural and Veterinary Engineering Department, ESAV, Polytechnic Institute of Viseu, Quinta da Alagoa , Viseu , 3504-510 , Portugal"},{"name":"CITAB, Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Tr\u00e1s-os-Montes e Alto Douro , Vila Real , Portugal"}]},{"given":"Elisabete","family":"Silva","sequence":"additional","affiliation":[{"name":"Environmental Department, ESTGV, Polytechnique Institute of Viseu, Campus Polit\u00e9cnico de Repeses , Viseu , 3504-510 , Portugal"},{"name":"Centre for Research in Digital Services (CISeD), Polytechnique Institute of Viseu , Viseu , 3504-510 , Portugal"},{"name":"LEPABE (Laboratory for Process Engineering, Environment, Biotechnology and Energy), FEUP, R. Dr. Roberto Frias , 4200-465 , Porto , Portugal"}]}],"member":"374","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"2022092113085808492_j_opag-2022-0120_ref_001","unstructured":"Anastas PT, Warner JC. Green chemistry: theory and practice. Oxford University Press; 1998. ISBN: 0198502346, 9780198502340."},{"key":"2022092113085808492_j_opag-2022-0120_ref_002","doi-asserted-by":"crossref","unstructured":"Hashem MA, Payel S, Hasan M, Momen MA, Sahariar MS. Green preservation of goatskin to deplete chloride from tannery wastewater. HighTech Innov J. 2021;2(2):99\u2013107. 10.28991\/HIJ-2021-02-02-0.","DOI":"10.28991\/HIJ-2021-02-02-03"},{"key":"2022092113085808492_j_opag-2022-0120_ref_003","unstructured":"Stevenson FJ. Humus Chemistry: genesis, composition, reactions. New York: John Wiley & Sons; 1994. ISBN: 978-0-471-59474-1."},{"key":"2022092113085808492_j_opag-2022-0120_ref_004","doi-asserted-by":"crossref","unstructured":"Janos P. 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Org Geochem, Kidlington. 1990;15:375\u201381. 10.1016\/0146-6380(90)90164-U.","DOI":"10.1016\/0146-6380(90)90164-U"},{"key":"2022092113085808492_j_opag-2022-0120_ref_008","unstructured":"Jodice R, Barberis R, Conciglis B, Ferrara R, Nappi P, Vicentino E. M\u00e9todo analitici: Fertilizanti organici, compost, fhangi di depurazione, riffute organici, sustrati in fermantazione metanica & biog\u00e1s. Instituto per el Piante da legno e l\u2019 Ambiente, Torino; 1984."},{"key":"2022092113085808492_j_opag-2022-0120_ref_009","unstructured":"Miller JN, Miller JC. Statistics and Chemometrics for Analytical Chemistry. England: Pearson Education Limited; 2010. ISBN: 978-0-273-73042-2."},{"key":"2022092113085808492_j_opag-2022-0120_ref_010","unstructured":"Leite F. Valida\u00e7\u00e3o em an\u00e1lise qu\u00edmica. Editora \u00c1tomo; 1996. ISBN: 978-85-7670-077-7."},{"key":"2022092113085808492_j_opag-2022-0120_ref_011","unstructured":"Welac & Eurachem. Accreditation for Chemical Laboratories: Guidance on the interpretation of the EN 45000 series of standards and ISO Guide 25. WELAC Guidance Document n. 2 & EURACHEM Guidance Document n. 1, Teddington; 1993."},{"key":"2022092113085808492_j_opag-2022-0120_ref_012","doi-asserted-by":"crossref","unstructured":"Rao TN. Validation of analytical methods. Intech Open Book Ser; 2017. 10.5772\/intechopen.72087.","DOI":"10.5772\/intechopen.72087"},{"key":"2022092113085808492_j_opag-2022-0120_ref_013","unstructured":"Eurachem. The Fitness for Purpose of Analytical Methods\u2009\u2013\u2009A Laboratory Guide to Method Validation and Related Topics. Eurachem; 2014. https:\/\/www.eurachem.org\/index.php\/publications\/guides\/mv."},{"key":"2022092113085808492_j_opag-2022-0120_ref_014","unstructured":"Real Decreto 1110\/1991. de 12 de jutia, por e\/quese \\ aprueban los m\u00e9todos oficiala. de\u00b7 an\u00e1lisis de productos org\u00e1nicos fertilizantes. Bol\u00e9tin Oficiale del Estado. 170, de 17 de julio de; 1991. p. 23725\u201330."},{"key":"2022092113085808492_j_opag-2022-0120_ref_015","unstructured":"Associa\u00e7\u00e3o de Laborat\u00f3rios Acreditados de Portugal. Guia RELACRE 13\u2009\u2013\u2009Valida\u00e7\u00e3o de M\u00e9todos Internos de Ensaio em An\u00e1lise Qu\u00edmica; 2000. ISBN: 972-8574-02-9. http:\/\/www.relacre.pt\/assets\/relacreassets\/files\/commissionsandpublications\/Guia%20RELACRE%2013.pdf."},{"key":"2022092113085808492_j_opag-2022-0120_ref_016","unstructured":"Portuguese Accreditation Institute. Guia para a Aplica\u00e7\u00e3o da NP EN ISO\/IEC 17025; 2018. http:\/\/www.ipac.pt\/docs\/publicdocs\/requisitos\/OGC001_GuiaAplicacao17025_v20181231.pdf."},{"key":"2022092113085808492_j_opag-2022-0120_ref_017","unstructured":"EPA. Green Chemistry. US EPA; 2013. https:\/\/www.epa.gov\/greenchemistry."},{"key":"2022092113085808492_j_opag-2022-0120_ref_018","unstructured":"Shamia IS, Halabi MN, El-Ashgar NM. Humic acid determination in some compost and fertilizer samples. IUG J Nat Stud. 2017;252:42\u201350. ISSN 2409-4587."},{"key":"2022092113085808492_j_opag-2022-0120_ref_019","doi-asserted-by":"crossref","unstructured":"Yang F, Tang C, Antonietti M. Natural and artificial humic substances to manage minerals, ions, water, and soil microorganisms. Chem Soc Rev. 2021;50:6221\u201339. 10.1039\/D0CS01363C.","DOI":"10.1039\/D0CS01363C"},{"key":"2022092113085808492_j_opag-2022-0120_ref_020","doi-asserted-by":"crossref","unstructured":"Olk DC, Dinnes DL, Scoresby JR, Callaway CR, Darlington JW. Humic products in agriculture: Potential benefits and research challenges\u2013A review. J Soil Sediment. 2018;18:2881\u201391. 10.1007\/s11368-018-1916-4.","DOI":"10.1007\/s11368-018-1916-4"},{"key":"2022092113085808492_j_opag-2022-0120_ref_021","doi-asserted-by":"crossref","unstructured":"Silva MEF, Lemos LT, Bastos MMSM, Nunes OC. Recovery of humic-like substances from low quality composts. 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