{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T21:22:19Z","timestamp":1777065739848,"version":"3.51.4"},"reference-count":34,"publisher":"Maad Rayan Publishing Company","issue":"4","content-domain":{"domain":["apb.tbzmed.ac.ir"],"crossmark-restriction":false},"short-container-title":["Adv Pharm Bull"],"abstract":"<jats:p>Purpose: Pancreatic adenocarcinoma has a high prevalence all over the world. Most of the therapeutic approaches failed as a result of tumor invasion and rapid metastasis. Several natural plants have been shown to have promising therapeutic effects. Thus, the aim of this study was to investigate the cytotoxic activity of Eryngium billardieri against PANC-1 cancer cell lines. Methods: Dimethylthiazole diphenyltetrazolium bromide assay (MTT assay) and \ufb02ow cytometry were used to assess the cytotoxicity of E. billardieri extracts against PANC-1 cancer cell lines. Quantitative Polymerase Chain Reaction (qPCR) was conducted to investigate the expression levels of Bcl2- associated X protein (BAX) and cyclin D1. Results: The results of the MTT assay showed that E. billardieri extracts had cytotoxic effects on PANC- 1 cancer cell lines. Moreover, the \ufb01ndings from the gene expression confirmed the over expression of Bax, and under expression of cyclin D1 following treatment with dichloromethane (DCM) and n-hexane (n- hex) extracts in cancer cells (P &lt; 0.05). Interestingly, the \ufb02ow cytometry results showed that DCM and n- hex extracts of E. billardieri induced apoptosis in PANC- 1 cancer cell lines. Conclusion: The results of this study demonstrated that DCM and n- hex extracts of E. billardieri significantly induce apoptosis by increasing Bax and decreasing cyclin D1 mRNA expression. Therefore, E. billardieri may be regarded as a novel approach for treatment of pancreatic cancer as a result of its promising apoptotic and cytotoxic properties.<\/jats:p>","DOI":"10.15171\/apb.2018.075","type":"journal-article","created":{"date-parts":[[2019,1,18]],"date-time":"2019-01-18T04:32:23Z","timestamp":1547785943000},"page":"667-674","update-policy":"https:\/\/doi.org\/10.15171\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Eryngium Billardieri Induces Apoptosis via Bax Gene Expression in Pancreatic Cancer Cells"],"prefix":"10.34172","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8173-6532","authenticated-orcid":true,"given":"Neda","family":"Roshanravan","sequence":"first","affiliation":[{"name":"Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9110-8209","authenticated-orcid":true,"given":"Parina","family":"Asgharian","sequence":"additional","affiliation":[{"name":"Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran."},{"name":"Department of Pharmacognosy, Tabriz University of Medical Sciences, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2477-9461","authenticated-orcid":true,"given":"Hassan","family":"Dariushnejad","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2563-8193","authenticated-orcid":true,"given":"Naimeh","family":"Mesri Alamdari","sequence":"additional","affiliation":[{"name":"Students Research Committee, School of Health, Iran University of Medical Science, Tehran, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9444-7134","authenticated-orcid":true,"given":"Behzad","family":"Mansoori","sequence":"additional","affiliation":[{"name":"Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4275-628X","authenticated-orcid":true,"given":"Ali","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2202-9598","authenticated-orcid":true,"given":"Shahriar","family":"Alipour","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Connective Tissue Disease Research Center, Tabriz University of Medical Sciences, Tabriz,Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2427-4615","authenticated-orcid":true,"given":"Meisam","family":"Barati","sequence":"additional","affiliation":[{"name":"Students Research Committee, Cellular and Molecular Nutrition Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6817-7812","authenticated-orcid":true,"given":"Abed","family":"Ghavami","sequence":"additional","affiliation":[{"name":"Nutrition Research Center, School of Nutrition, Tabriz University of Medical Science, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4195-3516","authenticated-orcid":true,"given":"Vajihe","family":"Ghorbanzadeh","sequence":"additional","affiliation":[{"name":"Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0794-2650","authenticated-orcid":true,"given":"Fatemeh","family":"Aamazadeh","sequence":"additional","affiliation":[{"name":"Nutrition Research Center, School of Nutrition, Tabriz University of Medical Science, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1058-1481","authenticated-orcid":true,"given":"Alireza","family":"Ostadrahimi","sequence":"additional","affiliation":[{"name":"Nutrition Research Center, School of Nutrition, Tabriz University of Medical Science, Tabriz, Iran."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"20123","published-online":{"date-parts":[[2018,11,29]]},"reference":[{"key":"R1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15841-8"},{"key":"R2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.29210"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-11-0332"},{"key":"R4","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1593\/neo.81686","article-title":"Gemcitabine-based chemogene therapy for pancreatic cancer using ad-dck:: Umk gdept and ts\/rr sirna strategies","volume":"11","author":"R\u00e9jiba S","year":"2009","journal-title":"Neoplasia"},{"key":"R5","doi-asserted-by":"publisher","DOI":"10.1179\/016164107X158965"},{"key":"R6","doi-asserted-by":"publisher","DOI":"10.1159\/000011879"},{"key":"R7","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1016\/S0046-8177(98)90244-1","article-title":"Overexpression of cyclin-d1, bcl-2, and bax proteins, proliferating cell nuclear antigen (pcna), and DNA-ploidy in squamous cell carcinoma of the oral cavity","volume":"29","author":"Staibano S","year":"1998","journal-title":"Hum Pathol"},{"key":"R8","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.06.021"},{"key":"R9","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-002-0435-5"},{"key":"R10","first-page":"78","article-title":"Urtica dioica dichloromethane extract induce apoptosis from intrinsic pathway on human prostate cancer cells (pc3)","volume":"62","author":"Mohammadi A","year":"2016","journal-title":"Cell Mol Biol (Noisy-le-grand)"},{"key":"R11","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/302426"},{"key":"R12","first-page":"62","article-title":"Effects of urtica dioica dichloromethane extract on cell apoptosis and related gene expression in human breast cancer cell line (MDA-Mb-468)","volume":"62","author":"Mohammadi A","year":"2016","journal-title":"Cell Mol Biol (Noisy-le-grand)"},{"key":"R13","doi-asserted-by":"publisher","DOI":"10.1080\/10412905.2004.9698648"},{"key":"R14","first-page":"43","article-title":"Cytotoxic activity of some ethnic medicinal plants from southwest of Iran","volume":"3","author":"Esmaeili S","year":"2016","journal-title":"Res J Pharmacogn"},{"key":"R15","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2650030111"},{"key":"R16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2006.02.005"},{"key":"R17","doi-asserted-by":"publisher","DOI":"10.1038\/srep21648"},{"key":"R18","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-013-0773-3"},{"key":"R19","first-page":"306","article-title":"In-vitro bioactivity and phytochemical screening of extracts from rhizomes of eremostachys azerbaijanica rech. F. Growing in iran","volume":"16","author":"Asnaashari S","year":"2017","journal-title":"Iran J Pharm Res"},{"key":"R20","doi-asserted-by":"publisher","DOI":"10.15171\/PS.2017.35"},{"key":"R21","doi-asserted-by":"publisher","DOI":"10.15171\/apb.2017.014"},{"key":"R22","first-page":"277","article-title":"Evaluation of various biological activities of the aerial parts of scrophularia frigida growing in iran","volume":"16","author":"Asgharian P","year":"2017","journal-title":"Iran J Pharm Res"},{"key":"R23","doi-asserted-by":"publisher","DOI":"10.22038\/IJBMS.2017.9554"},{"key":"R24","doi-asserted-by":"publisher","DOI":"10.5812\/jjnpp.36903"},{"key":"R25","doi-asserted-by":"publisher","DOI":"10.5812\/jjnpp.64312"},{"key":"R26","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"R27","doi-asserted-by":"publisher","DOI":"10.1186\/s40199-015-0136-3"},{"key":"R28","first-page":"211","article-title":"Cell cycle control in mammalian cells: Role of cyclins, cyclin dependent kinases (cdks), growth suppressor genes and cyclin-dependent kinase inhibitors (ckis)","volume":"11","author":"Gra\u00f1a X","year":"1995","journal-title":"Oncogene"},{"key":"R29","first-page":"63","article-title":"Cyclins and oncogenesis","volume":"1155","author":"Motokura T","year":"1993","journal-title":"Biochim Biophys Acta"},{"key":"R30","doi-asserted-by":"publisher","DOI":"10.1038\/35082500"},{"key":"R31","first-page":"1634","article-title":"Overexpression of cyclin d1 in human pancreatic carcinoma is associated with poor prognosis","volume":"57","author":"Gansauge S","year":"1997","journal-title":"Cancer Res"},{"key":"R32","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1083\/jcb.139.5.1281","article-title":"Movement of Bax from the Cytosol to Mitochondria during Apoptosis","volume":"139","author":"Wolter KG","year":"1997","journal-title":"J Cell Biol"},{"key":"R33","doi-asserted-by":"crossref","first-page":"99","DOI":"10.2174\/2210290601203010099","article-title":"Phytochemical constituents and pharmacological activities of eryngium l.(apiaceae)","volume":"3","author":"Wang P","year":"2012","journal-title":"pharma crops"},{"key":"R34","doi-asserted-by":"publisher","DOI":"10.1021\/np060101w"}],"container-title":["Advanced Pharmaceutical Bulletin"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/apb.tbzmed.ac.ir\/PDF\/apb-20746","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/apb.tbzmed.ac.ir\/PDF\/apb-20746","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T16:11:03Z","timestamp":1581696663000},"score":1,"resource":{"primary":{"URL":"https:\/\/apb.tbzmed.ac.ir\/Abstract\/apb-20746"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,29]]},"references-count":34,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2018,11]]}},"URL":"https:\/\/doi.org\/10.15171\/apb.2018.075","relation":{},"ISSN":["2228-5881","2251-7308"],"issn-type":[{"value":"2228-5881","type":"print"},{"value":"2251-7308","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,29]]},"assertion":[{"value":"Tabriz University of Medical Sciences","name":"journal_owner","label":"Journal Owner"},{"value":"Tabriz University of Medical Sciences","name":"journal_publisher","label":"Journal Publisher"}]}}