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These differences are likely to correspond with age-related silencing of proteins necessary for axon growth and elongation. In previous studies, it has been shown that reintroduction of the \u03b19 integrin subunit (tenascin-C receptor, \u03b19) that is downregulated in adult CNS can improve neurite outgrowth and sensory axon regeneration after a dorsal rhizotomy or a dorsal column crush spinal cord lesion. In the current study, we demonstrate that virally expressed integrins (\u03b19, \u03b16, or \u03b21 integrin) in the adult rat sensorimotor cortex and adult red nucleus are excluded from axons following neuronal transduction. Attempts to stimulate transport by inclusion of a cervical spinal injury and thus an upregulation of extracellular matrix molecules at the lesion site, or cotransduction with its binding partner, \u03b21 integrin, did not induce integrin localization within axons. In contrast, virally expressed \u03b19 integrin in developing rat cortex (postnatal day 5 or 10) demonstrated clear localization of integrins in cortical axons revealed by the presence of integrin in the axons of the corpus callosum and internal capsule, as well as in the neuronal cell body. Furthermore, examination of dorsal root ganglia neurons and retinal ganglion cells demonstrated integrin localization both within peripheral nerve as well as dorsal root axons and within optic nerve axons, respectively. Together, our results suggest a differential ability for<jats:italic>in vivo<\/jats:italic>axonal transport of transmembrane proteins dependent on neuronal age and subtype.<\/jats:p>","DOI":"10.1523\/eneuro.0029-16.2016","type":"journal-article","created":{"date-parts":[[2016,7,8]],"date-time":"2016-07-08T01:03:38Z","timestamp":1467939818000},"page":"ENEURO.0029-16.2016","source":"Crossref","is-referenced-by-count":45,"title":["Axonal Localization of Integrins in the CNS Is Neuronal Type and Age Dependent"],"prefix":"10.1523","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5960-5619","authenticated-orcid":false,"given":"Melissa R.","family":"Andrews","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Soleman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1659-3771","authenticated-orcid":false,"given":"Menghon","family":"Cheah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5169-0561","authenticated-orcid":false,"given":"David A.","family":"Tumbarello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew R. 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