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To study the functional consequences of voltage-gated sodium channel mutations, we use zebrafish with a loss-of-function mutation in<jats:italic>scn1lab<\/jats:italic>, a zebrafish homolog of human<jats:italic>SCN1A<\/jats:italic>. Homozygous<jats:italic>scn1lab<\/jats:italic><jats:sup>s552\/s552<\/jats:sup>mutants exhibit early-life seizures, metabolic deficits, and early death. Here, we developed<jats:italic>in vivo<\/jats:italic>assays using<jats:italic>scn1lab<\/jats:italic><jats:sup>s552<\/jats:sup>mutants between 3 and 6 d postfertilization (dpf). To evaluate sleep disturbances, we monitored larvae for 24 h with locomotion tracking software. Locomotor activity during dark (night phase) was significantly higher in mutants than in controls. Among anticonvulsant drugs, clemizole and diazepam, but not trazodone or valproic acid, decreased distance moved at night for<jats:italic>scn1lab<\/jats:italic><jats:sup>s552<\/jats:sup>mutant larvae. To monitor exploratory behavior in an open field, we tracked larvae in a novel arena. Mutant larvae exhibited impaired exploratory behavior, with increased time spent near the edge of the arena and decreased mobility, suggesting greater anxiety. Both clemizole and diazepam, but not trazodone or valproic acid, decreased distance moved and increased time spent in the center of the arena. Counting inhibitory neurons<jats:italic>in vivo<\/jats:italic>revealed no differences between<jats:italic>scn1lab<\/jats:italic><jats:sup>s552<\/jats:sup>mutants and siblings. Taken together, our results demonstrate conserved features of sleep, anxiety, and movement disorders in<jats:italic>scn1lab<\/jats:italic>mutant zebrafish, and provide evidence that a zebrafish model allows effective tests of treatments for behavioral comorbidities associated with DS.<\/jats:p>","DOI":"10.1523\/eneuro.0066-17.2017","type":"journal-article","created":{"date-parts":[[2017,8,4]],"date-time":"2017-08-04T00:40:35Z","timestamp":1501807235000},"page":"ENEURO.0066-17.2017","source":"Crossref","is-referenced-by-count":52,"title":["Behavioral Comorbidities and Drug Treatments in a Zebrafish<i>scn1lab<\/i>Model of Dravet Syndrome"],"prefix":"10.1523","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3737-2724","authenticated-orcid":false,"given":"Brian P.","family":"Grone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5132-4484","authenticated-orcid":false,"given":"Tiange","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1975-9781","authenticated-orcid":false,"given":"Scott C.","family":"Baraban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2017,8,3]]},"reference":[{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2013.09.009"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.beproc.2012.10.014"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.3","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3410"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.11.031"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.23.10545"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.6","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2097"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.7","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1242\/jeb.203.17.2565","article-title":"Locomotor repertoire of the larval zebrafish: swimming, turning and prey capture","volume":"203","year":"2000","journal-title":"J Exp Biol"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.8","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199810260-00020"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-011112-140232"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2011.03244.x"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.11","doi-asserted-by":"publisher","DOI":"10.1086\/320609"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.12","doi-asserted-by":"publisher","DOI":"10.1038\/nature14135"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.13","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2008.062323"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2014.01.017"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.15","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)66786-4"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.16","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0068-15.2015"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.17","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2011.02994.x"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2008.06.008"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.19","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2013.00009"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.20","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(15)00199-4"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.21","doi-asserted-by":"publisher","DOI":"10.1038\/74159"},{"key":"2023041302120637000_4.4.ENEURO.0066-17.2017.22","doi-asserted-by":"crossref","unstructured":"Fero K , Yokogawa T , Burgess HA (2011) The behavioral repertoire of larval zebrafish. 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