{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T11:18:13Z","timestamp":1774264693293,"version":"3.50.1"},"reference-count":60,"publisher":"Society for Neuroscience","issue":"9","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"vor","delay-in-days":181,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"funder":[{"name":"The Research Funds of Oujiang Laboratory"},{"name":"The Fundamental Research Funds of State Key Laboratory of Ophthalmology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["eNeuro"],"accepted":{"date-parts":[[2024,9,3]]},"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:p>Ca<jats:sup>2+<\/jats:sup>-binding proteins (CaBPs; CaBP1\u20135) are a subfamily of neuronal Ca<jats:sup>2+<\/jats:sup>sensors with high homology to calmodulin. Notably, CaBP4, which is exclusively expressed in rod and cone photoreceptors, is crucial for maintaining normal retinal functions. However, the functional roles of CaBP1, CaBP2, and CaBP5 in the retina remain elusive, primarily due to limited understanding of their expression patterns within inner retinal neurons. In this study, we conducted a comprehensive transcript analysis using single-cell RNA sequencing datasets to investigate the gene expression profiles of CaBPs in mouse and human retinal neurons. Our findings revealed notable similarities in the overall expression patterns of CaBPs across both species. Specifically, nearly all amacrine cell, ganglion cell, and horizontal cell types exclusively expressed CaBP1. In contrast, the majority of bipolar cell types, including rod bipolar (RB) cells, expressed distinct combinations of CaBP1, CaBP2, and CaBP5, rather than a single CaBP as previously hypothesized. Remarkably, mouse rods and human cones exclusively expressed CaBP4, whereas mouse cones and human rods coexpressed both CaBP4 and CaBP5. Our single-cell reverse transcription polymerase chain reaction analysis confirmed the coexpression CaBP1 and CaBP5 in individual RBs from mice of either sex. Additionally, all three splice variants of CaBP1, primarily L-CaBP1, were detected in mouse RBs. Taken together, our study offers a comprehensive overview of the distribution of CaBPs in mouse and human retinal neurons, providing valuable insights into their roles in visual functions.<\/jats:p>","DOI":"10.1523\/eneuro.0145-24.2024","type":"journal-article","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T17:50:21Z","timestamp":1726077021000},"page":"ENEURO.0145-24.2024","source":"Crossref","is-referenced-by-count":2,"title":["Comprehensive Characterization of a Subfamily of Ca<sup>2+<\/sup>-Binding Proteins in Mouse and Human Retinal Neurons at Single-Cell 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