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In most neurons, the synaptic targeting, pharmacology, and function of AMPARs are influenced by auxiliary subunits including the transmembrane AMPA receptor regulatory proteins (TARPs). However, it is unclear which TARP subunits are present at retinal synapses and how they influence receptor localization and function. Here, we show that TARP\u02632 (stargazin) is associated with AMPARs in the synaptic layers of the mouse, rabbit, macaque, and human retina. In most species, TARP\u02632 expression was high where starburst amacrine cells (SACs) ramify and transcriptomic analyses suggest correspondingly high gene expression in mouse and human SACs. Synaptic expression of GluA2, GluA3, and GluA4 was significantly reduced in a mouse mutant lacking TARP\u02632 expression (stargazer mouse;<jats:italic>stg<\/jats:italic>), whereas GluA1 levels were unaffected. AMPAR-mediated light-evoked EPSCs in ON-SACs from<jats:italic>stg<\/jats:italic>mice were \u223c30% smaller compared with heterozygous littermates. There was also loss of a transient ON pathway-driven GABAergic input to ON-SACs in<jats:italic>stg<\/jats:italic>mutants. Direction-selective ganglion cells in the<jats:italic>stg<\/jats:italic>mouse showed normal directional tuning, but their surround inhibition and thus spatial tuning was reduced. Our results indicate that TARP\u02632 is required for normal synaptic expression of GluA2, GluA3, and GluA4 in the inner retina. The presence of residual AMPAR expression in the stargazer mutant suggests that other TARP subunits may compensate in the absence of TARP\u02632.<\/jats:p>","DOI":"10.1523\/eneuro.0158-23.2023","type":"journal-article","created":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T17:50:14Z","timestamp":1690307414000},"page":"ENEURO.0158-23.2023","source":"Crossref","is-referenced-by-count":1,"title":["TARP\u03b32 Is Required for Normal AMPA Receptor Expression and Function in Direction-Selective Circuits of the Mammalian Retina"],"prefix":"10.1523","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7504-2422","authenticated-orcid":false,"given":"Todd","family":"Stincic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline","family":"Gayet-Primo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6806-3249","authenticated-orcid":false,"given":"W. 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