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Increased hypersomnia, fatigue, and surgical complications associated with general anesthesia suggest possible sensitivity to GABAergic inhibition in DM1. To test the hypothesis that MBNL2 depletion leads to behavioral sensitivity to GABA<jats:sub>A<\/jats:sub>receptor (GABA<jats:sub>A<\/jats:sub>-R) modulation,<jats:italic>Mbnl2<\/jats:italic>knock-out (KO) and wild-type (WT) littermates were treated with the anesthetic sevoflurane, the benzodiazepine diazepam, the imidazopyridine zolpidem, and the benzodiazepine rescue agent, flumazenil (Ro 15-1788), and assessed for various behavioral metrics.<jats:italic>Mbnl2<\/jats:italic>KO mice exhibited delayed recovery following sevoflurane, delayed emergence and recovery from zolpidem, and enhanced sleep time at baseline that was modulated by flumazenil. A significantly higher proportion of<jats:italic>Mbnl2<\/jats:italic>KO mice also loss their righting reflex [loss of righting reflex (LORR)] from a standard diazepam dose. We further examined whether MBNL2 depletion affects total GABA<jats:sub>A<\/jats:sub>-R mRNA subunit levels and validated RNA-sequencing data of mis-spliced<jats:italic>Gabrg2<\/jats:italic>, whose isoform ratios are known to regulate GABA sensitivity and associated behaviors. While no other GABA<jats:sub>A<\/jats:sub>-R subunit mRNA levels tested were altered in<jats:italic>Mbnl2<\/jats:italic>KO mouse prefrontal cortex,<jats:italic>Gabrg2S\/L<\/jats:italic>mRNA ratio levels were significantly altered. Taken together, our findings indicate that loss of MBNL2 function affects GABAergic function in a mouse model of myotonic dystrophy (DM1).<\/jats:p>","DOI":"10.1523\/eneuro.0218-22.2022","type":"journal-article","created":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T17:52:20Z","timestamp":1663955540000},"page":"ENEURO.0218-22.2022","source":"Crossref","is-referenced-by-count":6,"title":["Altered Behavioral Responses Show GABA Sensitivity in Muscleblind-Like 2-Deficient Mice: Implications for CNS Symptoms in Myotonic Dystrophy"],"prefix":"10.1523","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4408-6988","authenticated-orcid":false,"given":"Kamyra S.","family":"Edokpolor","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anwesha","family":"Banerjee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zachary 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