{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:53:06Z","timestamp":1792788786731,"version":"4.5.0"},"reference-count":51,"publisher":"Society for Neuroscience","issue":"3","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000098","name":"Stem Cell Network","doi-asserted-by":"crossref","award":["501100000098"],"award-info":[{"award-number":["501100000098"]}],"id":[{"id":"10.13039\/501100000098","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Alberta Innovations Health Solutions CRIO","award":["201200859"],"award-info":[{"award-number":["201200859"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["eNeuro"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Despite its modest capacity for regeneration, peripheral nervous system injury often results in significant long-term disability. Supplementing peripheral nervous system injury with autologous Schwann cells (SCs) may serve to rejuvenate the postinjury environment to enhance regeneration and ultimately improve functional outcomes. However, human nerve-derived SC (hN-SC) collection procedures require invasive surgical resection. Here, we describe the characterization of SCs from adult human skin (hSk-SCs) of four male donors ranging between 27 and 46 years old. Within five weeks of isolating and culturing adherent mixed skin cells, we were able to obtain 3\u20135 million purified SCs. We found that hSk-SCs appeared transcriptionally indistinguishable from hN-SCs with both populations exhibiting expression of SC genes including:\n                    <jats:italic>SOX10<\/jats:italic>\n                    ,\n                    <jats:italic>SOX9<\/jats:italic>\n                    ,\n                    <jats:italic>AP2A1<\/jats:italic>\n                    ,\n                    <jats:italic>CDH19<\/jats:italic>\n                    ,\n                    <jats:italic>EGR1<\/jats:italic>\n                    ,\n                    <jats:italic>ETV5<\/jats:italic>\n                    ,\n                    <jats:italic>PAX3<\/jats:italic>\n                    ,\n                    <jats:italic>SOX2<\/jats:italic>\n                    ,\n                    <jats:italic>CX32<\/jats:italic>\n                    ,\n                    <jats:italic>DHH<\/jats:italic>\n                    ,\n                    <jats:italic>NECL4<\/jats:italic>\n                    ,\n                    <jats:italic>NFATC4<\/jats:italic>\n                    ,\n                    <jats:italic>POU3F1<\/jats:italic>\n                    ,\n                    <jats:italic>S100B<\/jats:italic>\n                    , and\n                    <jats:italic>YY1<\/jats:italic>\n                    . Phenotypic analysis of hSk-SCs and hN-SCs cultures revealed highly enriched populations of SCs indicated by the high percentage of NES\n                    <jats:sup>+ve<\/jats:sup>\n                    , SOX10\n                    <jats:sup>+ve<\/jats:sup>\n                    , s100\n                    <jats:sup>+ve<\/jats:sup>\n                    and p75\n                    <jats:sup>+ve<\/jats:sup>\n                    cells, as well as the expression of a battery of other SC-associated proteins (PAX3, CDH19, ETV5, SOX2, POU3F1, S100B, EGR2, and YY1). We further show that both hSk-SCs and hN-SCs are capable of promoting axonal growth to similar degrees and that a subset of both associate with regenerating axons and form myelin following transplantation into the injured mouse sciatic nerve. Interestingly, although the majority of both hSk-SCs and hN-SCs maintained SOX10 immunoreactivity following transplant, only a subset of each activated the promyelinating factor, POU3F1, and were able to myelinate. Taken together, we demonstrate that adult hSk-SCs are genetically and phenotypically indistinguishable to hN-SCs.\n                  <\/jats:p>","DOI":"10.1523\/eneuro.0307-16.2017","type":"journal-article","created":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T20:55:22Z","timestamp":1493758522000},"page":"ENEURO.0307-16.2017","source":"Crossref","is-referenced-by-count":58,"title":["Purification and Characterization of Schwann Cells from Adult Human Skin and Nerve"],"prefix":"10.1523","volume":"4","author":[{"given":"Jo 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