{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:36:27Z","timestamp":1740173787609,"version":"3.37.3"},"reference-count":29,"publisher":"Society for Neuroscience","issue":"11","license":[{"start":{"date-parts":[[2024,5,1]],"date-time":"2024-05-01T00:00:00Z","timestamp":1714521600000},"content-version":"vor","delay-in-days":182,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["eNeuro"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The retina has diverse neuronal cell types derived from a common pool of retinal progenitors. Many molecular drivers, mostly transcription factors, have been identified to promote different cell fates. In<jats:italic>Drosophila<\/jats:italic>,<jats:italic>atonal<\/jats:italic>is required for specifying photoreceptors. In mice, there are two closely related<jats:italic>atonal<\/jats:italic>homologs,<jats:italic>Atoh1<\/jats:italic>and<jats:italic>Atoh7<\/jats:italic>. While<jats:italic>Atoh7<\/jats:italic>is known to promote the genesis of retinal ganglion cells, there is no study on the function of<jats:italic>Atoh1<\/jats:italic>in retinal development. Here, we crossed<jats:italic>Atoh1<jats:sup>Cre\/+<\/jats:sup><\/jats:italic>mice to mice carrying a Cre-dependent TdTomato reporter to track potential<jats:italic>Atoh1<\/jats:italic>-lineage neurons in retinas. We characterized a heterogeneous group of TdTomato<jats:sup>+<\/jats:sup>retinal neurons that were detected at the postnatal stage, including glutamatergic amacrine cells, AII amacrine cells, and BC3b bipolar cells. Unexpectedly, we did not observe TdTomato<jats:sup>+<\/jats:sup>retinal neurons in the mice with an<jats:italic>Atoh1-FlpO<\/jats:italic>knock-in allele and a Flp-dependent TdTomato reporter, suggesting<jats:italic>Atoh1<\/jats:italic>is not expressed in the mouse retina. Consistent with these data, conditional removal of<jats:italic>Atoh1<\/jats:italic>in the retina did not cause any observable phenotypes. Importantly, we did not detect<jats:italic>Atoh1<\/jats:italic>expression in the retina at multiple ages using mice with<jats:italic>Atoh1-GFP<\/jats:italic>knock-in allele. Therefore, we conclude that<jats:italic>Atoh1<jats:sup>Cre\/+<\/jats:sup><\/jats:italic>mice have ectopic Cre expression in the retina and that<jats:italic>Atoh1<\/jats:italic>is not required for retinal development.<\/jats:p>","DOI":"10.1523\/eneuro.0307-23.2023","type":"journal-article","created":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T17:56:05Z","timestamp":1699034165000},"page":"ENEURO.0307-23.2023","source":"Crossref","is-referenced-by-count":0,"title":["The<i>Atoh1-Cre<\/i>Knock-In Allele Ectopically Labels a Subpopulation of Amacrine Cells and Bipolar Cells in Mouse Retina"],"prefix":"10.1523","volume":"10","author":[{"given":"Sih-Rong","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0700-3349","authenticated-orcid":false,"given":"Huda 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