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The major cellular components of the NH are hypothalamic axonal termini, fenestrated endothelia and pituicytes, the resident astroglia. However, despite the physiological importance of the NH, the exact molecular signature defining neurohypophyseal cell types and in particular the pituicytes, remains unclear. Using single-cell RNA sequencing (scRNA-Seq), we captured seven distinct cell types in the NH and intermediate lobe (IL) of adult male mouse. We revealed novel pituicyte markers showing higher specificity than previously reported. Bioinformatics analysis demonstrated that pituicyte is an astrocytic cell type whose transcriptome resembles that of tanycyte. Single molecule\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization revealed spatial organization of the major cell types implying intercellular communications. We present a comprehensive molecular and cellular characterization of neurohypophyseal cell types serving as a valuable resource for further functional research.\n                  <\/jats:p>","DOI":"10.1523\/eneuro.0345-19.2019","type":"journal-article","created":{"date-parts":[[2020,1,8]],"date-time":"2020-01-08T17:45:21Z","timestamp":1578505521000},"page":"ENEURO.0345-19.2019","source":"Crossref","is-referenced-by-count":47,"title":["Single-Cell Molecular and Cellular Architecture of the Mouse Neurohypophysis"],"prefix":"10.1523","volume":"7","author":[{"given":"Qiyu","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dena","family":"Leshkowitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janna","family":"Blechman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3896-1881","authenticated-orcid":false,"given":"Gil","family":"Levkowitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2020,1,8]]},"reference":[{"key":"2023041302142129000_7.1.ENEURO.0345-19.2019.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.10.017"},{"key":"2023041302142129000_7.1.ENEURO.0345-19.2019.2","doi-asserted-by":"crossref","unstructured":"Bastian F , Parmentier G , Roux J , Moretti S , Laudet V , Robinson-Rechavi M (2008). 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