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Although enhancing PDGF-B signaling in pericytes represents a promising therapeutic strategy for ischemic stroke, practical approaches have not yet been developed. In this study, we evaluated the therapeutic efficacy and underlying mechanisms of PDGF-B-modified heat shock protein nanoparticles (PDGF-B HSPNPs) in a transient middle cerebral artery occlusion mouse model of either sex. Mice were treated with single intravenous injection of PDGF-B HSPNPs immediately after reperfusion, and infarct volume and motor function were assessed. To investigate cellular effects, flow cytometric analysis of brain-derived single\u2013cell suspensions was performed 3\u2005d after treatment to quantify platelet-derived growth factor receptor \u03b2-positive pericytes. Our results demonstrate that PDGF-B HSPNPs administration significantly reduced infarct volume and improved motor function, accompanied by enhanced activation of pericytes in the peri-infarct region. These findings suggest that PDGF-B HSPNPs represent a promising therapeutic approach for the treatment of ischemic stroke.<\/jats:p>","DOI":"10.1523\/eneuro.0404-25.2026","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T17:50:14Z","timestamp":1785261014000},"page":"ENEURO.0404-25.2026","update-policy":"https:\/\/doi.org\/10.1523\/eneuro.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic Potential of Platelet-Derived Growth Factor B-Modified Nanoparticles in Enhancing Pericyte Activation for Acute Ischemic 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