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Protocadherin-19 (<jats:italic>pcdh19<\/jats:italic>) is a homophilic cell adhesion molecule that is linked to neurodevelopmental disorders, and influences multiple cellular and developmental events in zebrafish. Although loss of<jats:italic>PCDH19<\/jats:italic>in humans and model organisms leads to functional deficits, the underlying network defects remain unknown. Here, we employ multiplane, resonant-scanning<jats:italic>in vivo<\/jats:italic>two-photon calcium imaging of developing zebrafish, and use graph theory to characterize the development of resting state functional networks in both wild-type and<jats:italic>pcdh19<\/jats:italic>mutant larvae. We find that the brain networks of<jats:italic>pcdh19<\/jats:italic>mutants display enhanced clustering and an altered developmental trajectory of network assembly. Our results show that functional imaging and network analysis in zebrafish larvae is an effective approach for characterizing the developmental impact of lesions in genes of clinical interest.<\/jats:p>","DOI":"10.1523\/eneuro.0420-18.2019","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T16:56:09Z","timestamp":1557248169000},"page":"ENEURO.0420-18.2019","source":"Crossref","is-referenced-by-count":13,"title":["Multiplane Calcium Imaging Reveals Disrupted Development of Network Topology in Zebrafish<i>pcdh19<\/i>Mutants"],"prefix":"10.1523","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4709-453X","authenticated-orcid":false,"given":"Sarah E.W.","family":"Light","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James D.","family":"Jontes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"393","published-online":{"date-parts":[[2019,5,6]]},"reference":[{"key":"2023041302134360000_6.3.ENEURO.0420-18.2019.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.03.040"},{"key":"2023041302134360000_6.3.ENEURO.0420-18.2019.2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2434"},{"key":"2023041302134360000_6.3.ENEURO.0420-18.2019.3","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000002704"},{"key":"2023041302134360000_6.3.ENEURO.0420-18.2019.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.06.056"},{"key":"2023041302134360000_6.3.ENEURO.0420-18.2019.5","doi-asserted-by":"crossref","unstructured":"Bisogni AJ , Ghazanfar S , Williams EO , Marsh HM , Yang JY , Lin DM (2018) Tuning of delta-protocadherin adhesion through combinatorial diversity. 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