{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T18:31:59Z","timestamp":1787596319488,"version":"build-2736575974"},"reference-count":54,"publisher":"Society for Neuroscience","issue":"8","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"vor","delay-in-days":184,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2020-04872"],"award-info":[{"award-number":["RGPIN-2020-04872"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["DGECR-2020-00030"],"award-info":[{"award-number":["DGECR-2020-00030"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eneuro.org"],"crossmark-restriction":true},"short-container-title":["eNeuro"],"accepted":{"date-parts":[[2026,7,27]]},"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:p>\n                    Cerebellar Purkinje cells (PCs) exhibit a unique and highly complex dendritic architecture, which plays a crucial role in integrating synaptic inputs and shaping their physiological properties. However, fine morphological investigation of PCs at the single-cell level is challenging due to the densely packed soma and extensive dendritic arborization. Here, we present stepwise procedures for the sparse labeling of PCs via a viral-mediated approach, enabling detailed two-dimensional dendritic Sholl analysis and three-dimensional spine quantification. We demonstrated high-resolution labeling of PCs through the administration of an adeno-associated virus (AAV) carrying a conditional Cre recombinase-dependent cassette (AAV,\n                    <jats:italic>CAG<\/jats:italic>\n                    -\n                    <jats:italic>FLEX-EGFP<\/jats:italic>\n                    ). The AAV was delivered through an intracerebroventricular injection in\n                    <jats:italic>Pcp2-Cre<\/jats:italic>\n                    neonatal mice of either sex. Subsequently, we optimized two analytical pipelines to characterize dendritic complexity and spines. As a sample application, we performed analysis on the labeled PCs from anterior (I\u2013V) and posterior (VI\u2013IX) cerebellar lobules in mice of both sexes. In doing so, we detected an age-dependent, lobule-specific dendritic branching pattern difference. PCs from the anterior lobules exhibited a higher spine density but a smaller spine head diameter compared with those from the posterior lobules. Additionally, we examined PC morphological perturbations in a disease model of Dravet syndrome, a disorder caused by\n                    <jats:italic>SCN1A<\/jats:italic>\n                    haploinsufficiency. Here, we identified reduced dendritic complexity and immature spine morphology in the PCs of\n                    <jats:italic>\n                      Scn1a\n                      <jats:sup>+\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice of both sexes. Thus, we provide a labeling method that is easily adaptable, efficient, robust, and well suited for examining PC morphology during cerebellar development and related pathogenic conditions.\n                  <\/jats:p>","DOI":"10.1523\/eneuro.0455-25.2026","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T17:50:27Z","timestamp":1785952227000},"page":"ENEURO.0455-25.2026","update-policy":"https:\/\/doi.org\/10.1523\/eneuro.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["A Streamlined Workflow for Purkinje Cell Labeling and High-Resolution Analyses of Dendrites and Spines in Mice"],"prefix":"10.1523","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1488-8421","authenticated-orcid":false,"given":"Xinzhu","family":"Tan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simeng","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Bai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Platon","family":"Megagiannis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Suresh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elias","family":"Pilianidis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Niibori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David 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