{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T06:53:53Z","timestamp":1793170433176,"version":"4.5.0"},"reference-count":40,"publisher":"Society for Neuroscience","issue":"6","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":183,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"funder":[{"DOI":"10.13039\/100002736","name":"Citizens United for Research in Epilepsy","doi-asserted-by":"publisher","award":["W81XWH-15-2-0069"],"award-info":[{"award-number":["W81XWH-15-2-0069"]}],"id":[{"id":"10.13039\/100002736","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["eNeuro"],"accepted":{"date-parts":[[2025,5,19]]},"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:p>Animal studies and human tissue experiments have demonstrated that traumatic brain injury (TBI) causes damage to the extracellular matrix (ECM). To test the hypothesis that TBI causes disruption of sulfated glycosaminoglycan (sGAG) in the ECM, we measured levels of sGAG in the cerebrospinal fluid (CSF), blood, and urine, in patients with severe TBI in the acute postinjury period. Samples of CSF, blood, and urine were obtained within 72\u2005h of injury in patients who received external ventricular drains as part of their treatment of severe TBI. Levels of chondroitin and heparan sGAGs were measured, along with their disaccharide constituents. Demographic information, presence of polytrauma, brain injury load, and distance of radiologically visible parenchymal injury from the ventricle were analyzed for correlation with total subtype sGAG levels. Levels were measured in 14 patients ranging in age from 17 to 90 years. CSF sGAG levels were variable among patients, with higher sGAG levels in plasma compared with CSF. Patients with polytrauma had nonsignificantly higher blood sGAG compared with patients with isolated head injury. Subcategories of CSF sGAG levels correlated with distance from the ventricle of parenchymal injury but not with brain injury load. This study is the first to measure sGAG levels in ventricular CSF and the first to analyze levels in TBI. These data demonstrate the elevation locally of intracranial sGAGs after severe TBI and suggest rapid local metabolism of these breakdown products. The consequences of ECM breakdown may provide unique therapeutic and preventive avenues to mitigate postinjury sequelae.<\/jats:p>","DOI":"10.1523\/eneuro.0488-24.2025","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T13:50:21Z","timestamp":1749563421000},"page":"ENEURO.0488-24.2025","source":"Crossref","is-referenced-by-count":2,"title":["Release of Extracellular Matrix Components after Human Traumatic Brain Injury"],"prefix":"10.1523","volume":"12","author":[{"given":"Michael","family":"Bambrick","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0949-9437","authenticated-orcid":false,"given":"Deena","family":"Godfrey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark D.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey D.","family":"Esko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biswa","family":"Choudhury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Gomez Toledo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mousumi","family":"Paulchakrabarti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Fortes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3072-518X","authenticated-orcid":false,"given":"Kevin J.","family":"Staley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann-Christine","family":"Duhaime","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"393","published-online":{"date-parts":[[2025,6,10]]},"reference":[{"key":"2025062610501619000_12.6.ENEURO.0488-24.2025.1","doi-asserted-by":"crossref","first-page":"6106","DOI":"10.1007\/s12035-015-9520-8","article-title":"Role of matrix metalloproteinases in the pathogenesis of traumatic brain injury","volume":"53","author":"Abdul-Muneer","year":"2016","journal-title":"Mol Neurobiol"},{"key":"2025062610501619000_12.6.ENEURO.0488-24.2025.2","doi-asserted-by":"crossref","first-page":"e222","DOI":"10.1097\/SLA.0000000000004113","article-title":"The impact of COVID-19 on clinical trials","volume":"272","author":"Asaad","year":"2020","journal-title":"Ann Surg"},{"key":"2025062610501619000_12.6.ENEURO.0488-24.2025.3","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.ymgme.2018.08.003","article-title":"Anatomical changes and pathophysiology of the brain in mucopolysaccharidosis disorders","volume":"125","author":"Bigger","year":"2018","journal-title":"Mol Genet Metab"},{"key":"2025062610501619000_12.6.ENEURO.0488-24.2025.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2021.105334"},{"key":"2025062610501619000_12.6.ENEURO.0488-24.2025.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2013.12.024"},{"key":"2025062610501619000_12.6.ENEURO.0488-24.2025.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmr.2010.07.238"},{"key":"2025062610501619000_12.6.ENEURO.0488-24.2025.7","doi-asserted-by":"crossref","unstructured":"Duhaime AC , Rindler R (2015) Special considerations in infants and children. 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