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Neurosci."],"published-print":{"date-parts":[[2022,10,26]]},"abstract":"<jats:p>Aquaporin-4 (AQP4) is characterized by the formation of orthogonal arrays of particles (OAPs) comprising its M1 and M23 isoforms in the plasma membrane. However, the biological importance of OAP formation is obscure. Here, we developed an OAP depolymerization male mouse model by transgenic knock-in of an AQP4-A25Q mutation. Analyses of the mutant brain tissue using blue native polyacrylamide gel electrophoresis, super-resolution imaging, and immunogold electron microscopy revealed remarkably reduced OAP structures and glial endfeet localization of the AQP4-A25Q mutant protein without effects on its overall mRNA and protein expression.<jats:italic>AQP4<jats:sup>A25Q\/A25Q<\/jats:sup><\/jats:italic>mice showed better survival and neurologic deficit scores when cerebral edema was induced by water intoxication or middle cerebral artery occlusion\/reperfusion. The brain water content and swelling of pericapillary astrocytic endfeet processes in<jats:italic>AQP4<jats:sup>A25Q\/A25Q<\/jats:sup><\/jats:italic>mice were significantly reduced, functionally supporting decreased AQP4 protein expression at the blood\u2013brain barrier. The infarct volume and neuronal damage were also reduced in<jats:italic>AQP4<jats:sup>A25Q\/A25Q<\/jats:sup><\/jats:italic>mice in the middle cerebral artery occlusion\/reperfusion model. Astrocyte activation in the brain was alleviated in<jats:italic>AQP4<jats:sup>A25Q\/A25Q<\/jats:sup><\/jats:italic>mice, which may be associated with decreased cell swelling. We conclude that the OAP structure of AQP4 plays a key role in its polarized expression in astrocytic endfeet processes at the blood\u2013brain barrier. Therefore, our study provided new insights into intervention of cerebral cellular edema caused by stroke and traumatic brain injury through regulating AQP4 OAP formation.<\/jats:p><jats:p><jats:bold>SIGNIFICANCE STATEMENT<\/jats:bold>Aquaporin-4 (AQP4) is characterized by orthogonal arrays of particles (OAPs) comprising the M1 and M23 isoforms in the membrane. Here, an OAP depolymerization male mouse model induced by AQP4-A25Q mutation was first established, and the functions of OAP depolymerization in cerebral edema have been studied. The results revealed that AQP4 lost its OAP structure without affecting AQP4 mRNA and protein levels in AQP4-A25Q mice. AQP4-A25Q mutation mice has neuroprotective effects on cerebral edema induced by water intoxication and middle cerebral artery occlusion\/reperfusion through relieving the activation of astrocytes and suppressed microglia-mediated neuroinflammation. We concluded that the OAP structure of AQP4 plays a key role in its polarized expression in astrocytic endfeet processes at the blood\u2013brain barrier. Therefore, our study provided new insights into intervention of cerebral cellular edema caused by stroke and traumatic brain injury through regulating AQP4 OAP formation.<\/jats:p>","DOI":"10.1523\/jneurosci.0401-22.2022","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T17:50:23Z","timestamp":1663091423000},"page":"8169-8183","source":"Crossref","is-referenced-by-count":23,"title":["AQP4-A25Q Point Mutation in Mice Depolymerizes Orthogonal Arrays of Particles and Decreases Polarized Expression of AQP4 Protein in Astrocytic Endfeet at the Blood\u2013Brain 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