{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T06:23:46Z","timestamp":1793946226970,"version":"4.8.0"},"reference-count":58,"publisher":"Society for Neuroscience","issue":"22","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"vor","delay-in-days":183,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurosci."],"published-print":{"date-parts":[[2016,6,1]]},"abstract":"<jats:p>Development of the hippocampal dentate gyrus (DG) in the mammalian brain is achieved through multiple processes during late embryonic and postnatal stages, with each developmental step being strictly governed by extracellular cues and intracellular mechanisms. Here, we show that the maintenance DNA methyltransferase 1 (Dnmt1) is critical for development of the DG in the mouse. Deletion of<jats:italic>Dnmt1<\/jats:italic>in neural stem cells (NSCs) at the beginning of DG development led to a smaller size of the granule cell layer in the DG. NSCs lacking<jats:italic>Dnmt1<\/jats:italic>failed to establish proper radial processes or to migrate into the subgranular zone, resulting in aberrant neuronal production in the molecular layer of the DG and a reduction of integrated neurons in the granule cell layer. Interestingly, prenatal deletion of<jats:italic>Dnmt1<\/jats:italic>in NSCs affected not only the developmental progression of the DG but also the properties of NSCs maintained into adulthood:<jats:italic>Dnmt1<\/jats:italic>-deficient NSCs displayed impaired neurogenic ability and proliferation. We also found that<jats:italic>Dnmt1<\/jats:italic>deficiency in NSCs decreased the expression of Reelin signaling components in the developing DG and increased that of the cell cycle inhibitors<jats:italic>p21<\/jats:italic>and<jats:italic>p57<\/jats:italic>in the adult DG. Together, these findings led us to propose that Dnmt1 functions as a key regulator to ensure the proper development of the DG, as well as the proper status of NSCs maintained into adulthood, by modulating extracellular signaling and intracellular mechanisms.<\/jats:p><jats:p><jats:bold>SIGNIFICANCE STATEMENT<\/jats:bold>Here, we provide evidence that Dnmt1 is required for the proper development of the hippocampal dentate gyrus (DG). Deletion of<jats:italic>Dnmt1<\/jats:italic>in neural stem cells (NSCs) at an early stage of DG development impaired the ability of NSCs to establish secondary radial glial scaffolds and to migrate into the subgranular zone of the DG, leading to aberrant neuronal production in the molecular layer, increased cell death, and decreased granule neuron production. Prenatal deletion of<jats:italic>Dnmt1<\/jats:italic>in NSCs also induced defects in the proliferation and neurogenic ability of adult NSCs. Furthermore, we found that Dnmt1 regulates the expression of key extracellular signaling components during developmental stages while modulating intracellular mechanisms for proliferation and neuronal production of NSCs in the adult.<\/jats:p>","DOI":"10.1523\/jneurosci.0512-16.2016","type":"journal-article","created":{"date-parts":[[2016,6,2]],"date-time":"2016-06-02T00:40:08Z","timestamp":1464828008000},"page":"6050-6068","source":"Crossref","is-referenced-by-count":41,"title":["DNA Methyltransferase 1 Is Indispensable for Development of the Hippocampal Dentate 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